Effects of cadmium on lipids of almond seedlings (Prunus dulcis)
Tóm tắt
Cadmium uptake and distribution, as well as its effects on lipid composition was investigated in almond seedlings (Prunus dulcis) grown in culture solution supplied with two concentrations of Cd (50 and 150 μM). The accumulation of Cd increased with external metal concentrations, and was considerably higher in roots than in leaves. Fourteen days after Cd treatment, the membrane lipids were extracted and separated on silica-gel thin layer chromatography (TLC). Fatty acid methyl esters were analyzed by FID-GC on a capillary column. Our results showed that Cd stress decreased the quantities of all lipids classes (phospholipids, galactolipids and neutral lipids). Galactolipid, phospholipid and neutral lipid concentrations decreased more in roots than in leaves by Cd-treatment. In almost all lipid classes the proportion of palmitic acid (16:0), linoleic (18: 2) and that of linolenic (18: 3) acid decreased, suggesting that heavy metal treatment induced an alteration in the fatty acid synthesis processes. In conclusion, our results show that the changes found in total fatty acids, in the quantities of all lipids classes, and in the in the profiles of individual polar lipids suggest that membrane structure and function might be altered by Cd stress.
Tài liệu tham khảo
Aidi Wannes W, Mhamdi B, Marzouk B (2009) Variations in essential oil and fatty acid composition during Myrtus communis var. italica fruit maturation. Food Chem 112:621–626 Aidi Wannes W, Mhamdi B, Marzouk B (2009) Variations in essential oil and fatty acid composition during Myrtus communis var. italica fruit maturation. Food Chem 112:621–626
Aronsson H, Schöttler MA, Kelly AA, Sundqvist C, Dörmann P, Karim S, Jarvis P: Monogalactosyldiacylglycerol deficiency in Arabidopsis affects pigment composition in the prolamellar body and impairs thylakoid membrane energization and photoprotection in leaves. Plant Physiol 2008, 148: 580–592. 10.1104/pp.108.123372
Attila H, Sára E, Tibor J, Erika T, Gábor H, Dénes D: Transgenic tobacco plants overproducing alfalfa aldose/aldehyde reductase show higher tolerance to low temperature and cadmium stress. Plant Sci 2004, 166: 1329–1333. 10.1016/j.plantsci.2004.01.013
Ben Ammar W, Nouairi I, Tray B, Zarrouk M, Jemal F, Ghorbel MH (2005) Effets du cadmium sur l’accumulation ionique et les teneurs en lipides dans les feuilles de tomate (Lycopersicon esculentum). J Soc Biol 199:157–163 Ben Ammar W, Nouairi I, Tray B, Zarrouk M, Jemal F, Ghorbel MH (2005) Effets du cadmium sur l’accumulation ionique et les teneurs en lipides dans les feuilles de tomate (Lycopersicon esculentum). J Soc Biol 199:157–163
Ben Youssef N, Nouairi I, Ben Temime S, Taamalli W, Zarrouk M, Ghorbel MH, Ben Miled Daoud D (2005) Effets du cadmium sur le métabolisme des lipides de plantules de colza (Brassica napus L.). C R Biol 328:745–757 Ben Youssef N, Nouairi I, Ben Temime S, Taamalli W, Zarrouk M, Ghorbel MH, Ben Miled Daoud D (2005) Effets du cadmium sur le métabolisme des lipides de plantules de colza (Brassica napus L.). C R Biol 328:745–757
Bligh EG, Dyer WJ: A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959, 37: 911–917. 10.1139/o59-099
Brenner RR: Effect of unsaturated acids on membrane structure and enzyme kinetics. Prog Lipid Res 1984, 23: 69–96. 10.1016/0163-7827(84)90008-0
Cecchi G, Biasini S, Castano J: Méthanolyse rapide des huiles en solvants. Note Lab Rev Franc Corps Gras 1985, 4: 163–164.
Certık M, Breierova E, Jursıkova P (2005) Effect of cadmium on lipid composition of Aureobasidium pullulans grown with added extracellular polysaccharides. Int Biodeter Biodegr 55:195–202 Certık M, Breierova E, Jursıkova P (2005) Effect of cadmium on lipid composition of Aureobasidium pullulans grown with added extracellular polysaccharides. Int Biodeter Biodegr 55:195–202
Chaffai R, Marzouk B, El Ferjani E: Aluminum mediates compositional alterations of polar lipid classes in maize seedlings. Phytochemistry 2005, 66: 1903–1912. 10.1016/j.phytochem.2005.06.028
Chaoui A, Mazhoudi S, Ghorbal MH, El Ferjani E (1997) Cadmium and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in bean (Phaseolus vulgaris L.). Plant Sci 127:139–147 Chaoui A, Mazhoudi S, Ghorbal MH, El Ferjani E (1997) Cadmium and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in bean (Phaseolus vulgaris L.). Plant Sci 127:139–147
Clijsters H, Van Assche F, Gora L: Physiological responses of higher plants to soil contamination with metals. In Ecological Responses to Environmental Stresses. Edited by: Rozema J, Verkleij JAC. Kluwer Academic Publishers, Netherlands; 1991:32–39. 10.1007/978-94-009-0599-3_4
Cobbett C, Goldsbrough P: Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis. Annu Rev Plant Physiol Plant Mol Biol 2002, 53: 159–182.
Das P, Samantaray S, Rout GR: Studies on cadmium toxicity in plants: a review. Environ Pollut 1997, 98: 29–36. 10.1016/S0269-7491(97)00110-3
De Vos CHR, Schat H: Free radicals and heavy metal tolerance, in Ecological responses to environmental stresses. In Ecological Responses to Environmental Stresses. Edited by: Rozema J, Verkleijv JAC. Kluwer Academic Publishers, Netherlands; 1991:22–33. 10.1007/978-94-009-0599-3_3
De Vos CHR, Ten Boukum WM, Vooijs R, Schat H, De Kok LJ (1993) Effect of copper on fatty acid composition and peroxidation of lipids in the roots of copper tolerant and sensitive Silene cucbalus. Plant Physiol Biochem 31:151–158 De Vos CHR, Ten Boukum WM, Vooijs R, Schat H, De Kok LJ (1993) Effect of copper on fatty acid composition and peroxidation of lipids in the roots of copper tolerant and sensitive Silene cucbalus. Plant Physiol Biochem 31:151–158
Di Toppi LS, Gabbrielli R: Response to cadmium in higher plants. Environ Exp Bot 1999, 41: 105–130. 10.1016/S0098-8472(98)00058-6
Djebali W, Chaïbi W, Ghorbel MH: Croissance, activité peroxydasique et modifications ultrastructurales induites par le cadmium dans la racine de tomate. Can J Bot 2002, 80: 942–953. 10.1139/b02-062
Djebali W, Zarrouk M, Brouquisse R, El Kahoui S, Limam F, Ghorbel MH, Chaïbi W (2005) Ultrastructure and lipid alterations induced by cadmium in tomato (Lycopersicon esculentum) chloroplast membranes. Plant Biol 7:258–268 Djebali W, Zarrouk M, Brouquisse R, El Kahoui S, Limam F, Ghorbel MH, Chaïbi W (2005) Ultrastructure and lipid alterations induced by cadmium in tomato (Lycopersicon esculentum) chloroplast membranes. Plant Biol 7:258–268
Elloumi N, Ben Abdallah F, Rhouman A, Ben Rwina B, Mezghani I, Boukhris M: Cadmium-induced growth inhibition and alteration of biochemical parameters in almond seedlings grown in solution culture. Acta Physiol Plant 2007, 29: 57–62.
Hall JL: Cellular mechanisms for heavy metal detoxification and tolerance. J Exp Bot 2002, 53: 1–11. 10.1093/jexbot/53.366.1
Halliwel B, Gutteridge JM: Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J 1984, 219: 1–14.
Hewitt EJ: Sand and water culture methods used in the study of plant nutrition. GAB Tech Gommun 1966, 22: 431–432.
Ivanov AG, Hendrickso L, Krol M, Selstam E, Oquis G, Hurry V, Huner NPA (2006) Digalactosyl-diacylglycerol deficiency impairs the capacity for photosynthetic intersystem electron transport and state transitions in Arabidopsis thaliana due to photosystem I acceptor-side limitations. Plant Cell Physiol 47:1146–1157 Ivanov AG, Hendrickso L, Krol M, Selstam E, Oquis G, Hurry V, Huner NPA (2006) Digalactosyl-diacylglycerol deficiency impairs the capacity for photosynthetic intersystem electron transport and state transitions in Arabidopsis thaliana due to photosystem I acceptor-side limitations. Plant Cell Physiol 47:1146–1157
Jemal F, Zarrouk M, Ghorbal MH: Effect of cadmium on lipid composition of pepper. Biochem Soc T 2000, 28: 907–910. 10.1042/BST0280907
Jordan P, Fromme P, Witt HT, Klukas O, Saenger W, Krauss N: Three-dimensional structure of cyanobacterial photosystem I at 2.5 A resolution. Nature 2001, 411: 909–917. 10.1038/35082000
Kennedy CD, Gonsalves FAN (1989) The action of divalent Zn, Cd, Hg, Cu and Pb ions on the ATPase activity of plasma membrane fraction isolated from roots of Zea mays. Plant Soil 117:167–175 Kennedy CD, Gonsalves FAN (1989) The action of divalent Zn, Cd, Hg, Cu and Pb ions on the ATPase activity of plasma membrane fraction isolated from roots of Zea mays. Plant Soil 117:167–175
Kobayashi K, Kondo M, Fukuda H, Nishimura M, Ohta H: Galactolipid synthesis in chloroplast inner envelope is essential for proper thylakoid biogenesis, photosynthesis, and embryogenesis. Proc Natl Acad Sci 2007, 104: 17216–17221. 10.1073/pnas.0704680104
Krantev A, Yordanova R, Janda T, Szalai G, Popova L: Treatment with salicylic acid decreases the effect of cadmium on photosynthesis in maize plants. J Plant Physiol 2008, 165: 920–931. 10.1016/j.jplph.2006.11.014
Le Guédard M, Schraauwers B, Larrieu I, Bessoule JJ: Development of a biomarker for metal bioavailability: the lettuce fatty acid composition. Environ Toxicol Chem 2008, 27: 1147–1151. 10.1897/07-277.1
Lepage M: Identification and composition of turnip root lipids. Lipids 1967, 2: 244–250. 10.1007/BF02532563
Loll B, Kern J, Seanger W, Zouni A, Biesiadka J: Lipids in photosystem II: Interactions with protein and cofactors. Biochim Biophys Acta 2007, 1767: 509–519. 10.1016/j.bbabio.2006.12.009
Ma JF, Ueno D, Zhao FJ, McGrath SP (2005) Subcellular localisation of Cd and Zn in the leaves of a Cd-hyperaccumulating ecotype of Thlaspi caerulescens. Planta 220:731–736 Ma JF, Ueno D, Zhao FJ, McGrath SP (2005) Subcellular localisation of Cd and Zn in the leaves of a Cd-hyperaccumulating ecotype of Thlaspi caerulescens. Planta 220:731–736
Maksymiec W, Russa R, Urbanik-Sypniewska T, Baszynski T: Changes in acyl lipid and fatty acid composition in thylakoids of copper non-tolerant spinach exposed to excess copper. J Plant Physiol 1992, 140: 52–55. 10.1016/S0176-1617(11)81056-4
Martin CE, Hiramitsu K, Kitajima Y, Nozawa Y, Skriver L, Thompson GA: Molecular control of membrane properties during temperature acclimation. Fatty acid desaturase regulation of membrane fluidity in acclimating Tetrahymena cells. Biochemistry 1976, 15: 5218–5227. 10.1021/bi00669a004
Meharg AA: The role of the plasmalemma in metal tolerance in angiosperms. Physiol Plantarum 1993, 88: 191–198. 10.1111/j.1399-3054.1993.tb01777.x
Mizusawa N, Wada H: The role of lipids in photosystem II. Biochim Biophys Acta (BBA)- Bioenergetics 2012, 1817: 194–208. 10.1016/j.bbabio.2011.04.008
Mohamed AA, Castagna A, Ranieri A, Sanità di Toppi L (2012) Cadmium tolerance in Brassica juncea roots and shoots is affected by antioxidant status and phytochelatin biosynthesis. Plant Physiol Biochem 57:15–22 Mohamed AA, Castagna A, Ranieri A, Sanità di Toppi L (2012) Cadmium tolerance in Brassica juncea roots and shoots is affected by antioxidant status and phytochelatin biosynthesis. Plant Physiol Biochem 57:15–22
Nouairi I, Ghnaya T, Ben Youssef N, Zarrouk M, Ghorbel MH: Changes in content and fatty acid profiles of total lipids of two halophytes: Sesuvium portulacastrum and Mesembryanthemum crystallinum under cadmium stress. J Plant Physiol 2006, 163: 1198–1202. 10.1016/j.jplph.2005.08.020
Nouairi I, Ben Ammar W, Ben Youssef N, Ben Miled Daoud D, Ghorbel MH, Zarrouk M (2006b) Comparative study of cadmium effects on membrane lipid composition of Brassica juncea and Brassica napus leaves. Plant Sci 130:165–170 Nouairi I, Ben Ammar W, Ben Youssef N, Ben Miled Daoud D, Ghorbel MH, Zarrouk M (2006b) Comparative study of cadmium effects on membrane lipid composition of Brassica juncea and Brassica napus leaves. Plant Sci 130:165–170
Nussbaum S, Schmutz D, Brunold C: Regulation of assimilatory sulfate reduction by cadmium in Zea mays L. Plant Physiol 1988, 88: 1407–1410. 10.1104/pp.88.4.1407
Ouariti O, Boussama N, Zarrouk M, Cherif A, Ghorbal MH: Cadmium- and copper-induced changes in tomato membrane lipids. Phytochemistry 1997, 45: 1343–1350. 10.1016/S0031-9422(97)00159-3
Ouzounidou G, Eleftheriou EP, Karataglis S (1992) Ecophysiological and ultrastructural effects of copper in Thlaspi ochroleucum (Cruciferae). Can J Bot 70:947–957 Ouzounidou G, Eleftheriou EP, Karataglis S (1992) Ecophysiological and ultrastructural effects of copper in Thlaspi ochroleucum (Cruciferae). Can J Bot 70:947–957
Robinson B, Russell C, Hedley M, Clothier B: Cadmium adsorption by rhizobacteria: implications for New Zealand pastureland. Agr Ecosyst Environ 2001, 87: 315–321. 10.1016/S0167-8809(01)00146-3
Schützendübel A, Schwanz P, Teichmann T, Gross K, Langenfeld-Heyser R, Godbold DL, Polle A: Cadmium-induced changes in antioxidative systems, hydrogen peroxide content, and differentiation in scots pine roots. Plant Physiol 2001, 127: 887–898. 10.1104/pp.010318
Shukla UC, Singh J, Joshi PC, Kakkar P: Effect of bioaccumulation of cadmium on biomass productivity, essential trace elements, chlorophyll biosynthesis, and macromolecules of wheat seedlings. Biol Trace Elem Res 2003, 92: 257–273. 10.1385/BTER:92:3:257
Skorzynska E, Urbanik-Sypniewska T, Russa R, Baszynski T: Galactolipase activity of chloroplasts in cadmium-treated runner bean plants. J Plant Physiol 1991, 138: 454–459. 10.1016/S0176-1617(11)80522-5
Sobkowiak R, Rymer K, Ruciniska R, Deckert J: Cadmium- induced changes in antioxidant enzyme in suspension culture of soybean cells. Acta Biochim Pol 2004, 51: 219–222.
Somashekaraiah BV, Padmaja K, Prasad ARK (1992) Phytotoxicity of cadmium ions on germinating seedlings of mung bean (Phaseolus vulgaris): Involvement of lipid peroxides in chlorphyll degradation. Physiol Plantarum 85:85–89 Somashekaraiah BV, Padmaja K, Prasad ARK (1992) Phytotoxicity of cadmium ions on germinating seedlings of mung bean (Phaseolus vulgaris): Involvement of lipid peroxides in chlorphyll degradation. Physiol Plantarum 85:85–89
Somerville C, Browse J: Plant lipids: metabolism, mutants, and membranes. Science 1991, 252: 80–87. 10.1126/science.252.5002.80
Stadman ER: Oxidation of free amino acids and amino acid residues in proteins by radiolysis and by metal-catalyzed reactions. Annu Rev Biochem 1993, 62: 797–821. 10.1146/annurev.bi.62.070193.004053
Sun YL, Li F, Su N, Sun XL, Zhao SJ, Meng QW: The increase in unsaturation of fatty acids of phosphatidylglycerol in thylakoid membrane enhanced salt tolerance in tomato. Photosynthetica 2010, 48: 400–408. 10.1007/s11099-010-0052-1
Vassilev A, Lidon F, Scotti P, Da Graca M, Yordanov I: Cadmium-induced changes in chloroplast lipids and photosystem activities in barley plants. Biol Plantarum 2004, 48: 153–156. 10.1023/B:BIOP.0000024295.27419.89
Velikova V, Tsonev T, Loreto F, Centritto M (2011) Changes in photosynthesis, mesophyll conductance to CO2, and isoprenoid emissions in Populus nigra plants exposed to excess nickel. Environ Pollut 159:1058–1066 Velikova V, Tsonev T, Loreto F, Centritto M (2011) Changes in photosynthesis, mesophyll conductance to CO2, and isoprenoid emissions in Populus nigra plants exposed to excess nickel. Environ Pollut 159:1058–1066
Verdoni N, Mench M, Cassagne C, Bessoule JJ: Fatty acid composition of tomato leaves as biomarkers of metal-contaminated soils. Environ Toxicol Chem 2001, 20: 382–388. 10.1002/etc.5620200220
Verkleij JAC, Schat H: Mechanisms of metal tolerance in higher plants. In Heavy Metal Tolerance in Plants: Evolutionary Aspects. Edited by: Shaw AJ. CRC Press, FL; 1990:179–193.
Wang SY, Lin HS (2006) Effect of plant growth temperature on membrane lipids in strawberry (Fragaria x ananassa Duch.). Sci Hortic Amsterdam 108:35–42 Wang SY, Lin HS (2006) Effect of plant growth temperature on membrane lipids in strawberry (Fragaria x ananassa Duch.). Sci Hortic Amsterdam 108:35–42
Williams JP, Khan MU, Mitchell K: Galactolipid biosynthesis in leaves of 16:3 and 18:3-plants. In Biosynthesis and function of plant lipids. Edited by: Thomson WW, Mudd JB, Gibbs M. American Society of Plant Physiology Symposium in Botany Riverside, California; 1983:28–39.
Xu XQ, Beardall J: Effect of salinity on fatty acid composition of a green microalga from an Antarctic hypersaline lake. Phytochemistry 1997, 45: 655–658. 10.1016/S0031-9422(96)00868-0
Zhang G, Slaski JJ, Archambault DJ, Taylor GJ (1996) Aluminum-induced alterations in lipid composition of microsomal membranes from an aluminum-resistant and aluminum-sensitive cultivar of Triticum aestivum. Physiol Plantarum 96:683–691 Zhang G, Slaski JJ, Archambault DJ, Taylor GJ (1996) Aluminum-induced alterations in lipid composition of microsomal membranes from an aluminum-resistant and aluminum-sensitive cultivar of Triticum aestivum. Physiol Plantarum 96:683–691