Effects of Tumour Necrosis Factor-α on Human Sperm Motility and Apoptosis

Springer Science and Business Media LLC - Tập 27 - Trang 152-162 - 2007
Anna Perdichizzi1, Ferdinando Nicoletti2, Sandro La Vignera1, Nunziata Barone1, Rosario D’Agata1, Enzo Vicari1, Aldo E. E. Calogero1,3
1Section of Endocrinology, Andrology and Internal Medicine, and Master in Andrological and Human Reproduction Sciences, University of Catania, Catania, Italy
2Department of Biomedical Sciences, University of Catania, Catania, Italy
3Sezione di Endocrinologia, Andrologia e Medicina Interna, Dipartimento di Scienze Biomediche, Università di Catania, Ospedale Garibaldi Centro, Catania, Italy

Tóm tắt

The aim of this study was to evaluate the effects of tumour necrosis factor-α (TNF-α) on sperm motility, mitochondrial membrane potential (ΔΨ), phosphatidylserine (PS) externalization, sperm chromatin packaging quality, and DNA fragmentation. Motile spermatozoa, obtained from 10 normozoospermic men, were incubated with increasing concentrations of TNF-α and analyzed 1, 3, 6, and 24 h after incubation by flow cytometry. TNF-α decreased total motility 24 h after incubation at 10 ng/mL and progressive motility 3 h after incubation. Accordingly, TNF-α reduced sperm ΔΨ in a concentration- and time-dependent manner. TNF-α increased the percentage of spermatozoa with PS externalization from the concentration of 1 ng/mL 1 h after incubation. TNF-α produced sperm chromatin and DNA damage in a concentration- and time-dependent manner. In conclusion, these findings may explain the reduction of fertility, secondary to upregulated production of TNF-α, in men with urogenital infections.

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