Repair of human sperm chromosome aberrations in the hamster egg

Springer Science and Business Media LLC - Tập 89 - Trang 181-186 - 1992
A. Genescà1, M. R. Caballín2, R. Miró1, J. Benet1, J. R. Germà3, J. Egozcue1
1Departament de Biologia Cel.lular i Fisiologia, Unitat de Biologia Cel.lular, Universitat Autónoma de Barcelona, Barcelona, Spain
2Departament de Biologia Animal, Biologia Vegetal i Ecologia Universitat Autónoma de Barcelona, Barcelona, Spain
3Servei d'Oncologia, Hospital de la Santa Creu i Sant Pau, Universitat Autónoma de Barcelona, Barcelona, Spain

Tóm tắt

In order to study the repair capacity of fertilized hamster eggs for the lesions present or induced in human sperm, we have examined the potentiating effect of caffeine, a DNA repair inhibitor, on the frequency and types of sperm chromosome aberrations. Sperm samples were donated by an individual treated with chemotherapy for a testicular cancer 3 years previously. Exposure of spermatozoa and inseminated oocytes to caffeine led to an increase of sperm chromosome aberrations, indicating that the damage to human sperm can be repaired in untreated hamster egg cytoplasm. The potentiating effect of caffeine was mainly reflected in an increase of unrejoined aberrations, indicating that the formation of chromosomal rearrangements is also inhibited. Since both chromatid-type and chromosome-type aberrations increase after treatment with caffeine, damage to human sperm can probably be repaired inside the hamster egg cytoplasm by pre and post-replication repair mechanisms.

Tài liệu tham khảo

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