Effects of Tumor Necrosis Factor‐Alpha on Human Trophoblast Cell Adhesion and Motility

American Journal of Reproductive Immunology - Tập 36 Số 2 - Trang 65-71 - 1996
Jill C. Todt1, Yan Yang2, Jun Lei2, Michele R. Lauria2, Yoram Sorokin2, David B. Cotton2, Frank D. Yelian2
1Department of Obstetrics & Gynecology, Wayne State University School of Medicine, Detroit, Michigan, USA.
2Department of Obstetrics and Gynecology Wayne State University School of Medicine Detroit Michigan

Tóm tắt

PROBLEM: Adhesive interaction between trophoblast cells and uterine endometrial basement membrane is one of the critical processes in embryo implantation. This interaction is directly or indirectly regulated by hormones, growth factors, and cytokines. Since tumor necrosis factor‐alpha (TNF‐α) is synthesized by both decidual and trophoblast cells, we hypothesized that TNF‐α may play a regulatory role in trophoblast cell invasion. To test this hypothesis, we have used in vitro models to determine the effect of TNF‐α on human trophoblast cell adhesion and motility, two major steps in trophoblast invasion.METHODS: The effect of TNF‐α on the motility of extended‐lifespan first trimester trophoblasts (HTR) and JEG‐3 choriocarcinoma cells was tested using the phagokinetic track motility assay. An in vitro adhesion assay was used to determine the effect of TNF‐α on the adhesion of HTR and JEG‐3 cells to laminin, a major basement membrane component. In addition, the effect of TNF‐α on the surface expression of the laminin receptor β1 integrin subunit was examined using flow cytometry.RESULTS: HTR or JEG‐3 cells were strongly adherent to laminin which was not significantly altered by TNF‐α treatment. We also measured the effect of TNF‐α on the surface expression of β1 integrin on HTR and JEG‐3 cells; no difference was observed between control and treatment groups. Interestingly, the motility of both HTR and choriocarcinoma JEG‐3 cells was significantly inhibited by TNF‐α.CONCLUSIONS: The role of TNF‐α in human embryo implantation is currently unknown. Our data demonstrate that TNF‐α does not alter trophoblast cell adhesion to laminin, but significantly inhibits trophoblast cell motility in vitro, suggesting that TNF‐α may play a regulatory role in trophoblast cell invasion.

Từ khóa


Tài liệu tham khảo

10.1007/978-1-4612-2716-8_12

10.3109/07853899309147870

10.1111/j.1749-6632.1994.tb21740.x

10.1111/j.1365-2249.1992.tb03059.x

Chen HL, 1991, Tumor necrosis factor alpha mRNA and protein are present in human placental and uterine cells at early and late stages of gestation, Am J Pathol, 139, 327

10.1172/JCI113901

Yelavarthi KK, 1993, Analysis of p60 and p80 tumor necrosis factor‐alpha receptor messenger RNA and protein in human placentas, Am J Pathol, 143, 1131

Hampson J., 1993, Low‐affinity receptors for tumour necrosis factor‐alpha, interferon‐gamma and granulocyte‐macrophage colony‐stimulating factor are expressed on human placental syncytiotrophoblast, Immunology, 79, 485

10.1146/annurev.bi.59.070190.004031

Vilcek J., 1991, Tumor necrosis factor: New insights into the molecular mechanisms of its multiple actions, J Biol Chem, 266, 7313, 10.1016/S0021-9258(20)89445-9

10.1084/jem.162.6.2163

10.1073/pnas.84.15.5277

10.1016/0008-8749(90)90088-9

10.1073/pnas.87.3.968

10.1210/en.131.1.14

Kupfermine MJ, 1994, Tumor necrosis factor‐α is elevated in plasma and amniotic fluid of patients with severe pre‐eclampsia, Am J Obstet Gynecol, 170, 1752, 10.1016/S0002-9378(12)91845-1

10.1111/j.1471-0528.1995.tb09020.x

Schrocksnadel H., 1994, Activated macrophages in the pathologic mechanism of pregnancy‐induced hypertension, Zentralbl Gynakol, 16, 274

Greer IA, 1994, Increased concentrations of cytokines interleukin‐6 and interleukin‐1 receptor antagonist in plasma of women with pre‐eclampsia: a mechanism for endothelial dysfunction, Obstet Gynecol, 84, 937

10.3109/00016349509009937

10.1111/j.1365-2249.1994.tb06615.x

10.1006/excr.1993.1139

10.1111/j.1365-2249.1991.tb08170.x

10.1016/0092-8674(77)90057-5

Watanabe H., 1991, The relationship between motility factor receptor internalization and the lung colonization capacity of murine melanoma cells, Cancer Res, 51, 2699

10.1006/excr.1995.1105

Albelda SM., 1993, Biology of Disease: Role of integrins and other cell adhesion molecules in tumor progression and metastasis, Lab Invest, 68, 4

10.1006/dbio.1994.1223

Steller MA, 1994, Effects of cytokines on epidermal growth factor receptor expression by malignant trophoblast cells in vitro, J Reprod Med, 39, 209

Hunt JS, 1990, Differential responses of rat trophoblast cells and embryonic fibroblast cells to cytokines that regulate proliferation and class MHC 1 antigen expression, J Immunol, 145, 184, 10.4049/jimmunol.145.1.184

10.1016/0002-9378(88)90810-1

Rosen EM, 1991, Tumor necrosis factor stimulates epithelial tumor cell motility, Cancer Res, 51, 5315

10.1210/jc.74.1.130

10.1530/eje.0.1310607

Santala P., 1991, Regulation of integrin‐type cell adhesion receptors by cytokines, J Biol Chem, 266, 23505, 10.1016/S0021-9258(18)54526-9

10.1074/jbc.270.7.3353

10.1016/0143-4004(95)90110-8

10.1038/icb.1993.5

Graham CH, 1992, Mechanisms of placental invasion of the uterus and their control. Biochem, Cell Biol., 70, 867

Librach CL, 1994, Interleukin‐1beta regulates human cytotrophoblast metalloproteinase activity and invasion in vitro, J Biol Chem, 269, 17125, 10.1016/S0021-9258(17)32529-2

Thông tin
Thông tin xuất bản

American Journal of Reproductive Immunology

Tập 36 Số 2

65-71

Thông tin tác giả