Effect of tocotrienols on the growth of a human breast cancer cell line in culture

Lipids - 1995
Kalanithi Nesaretnam1, Najla Guthrie2, Ann F. Chambers3, K. K. Carroll2
1Palm Oil Research Institute of Malaysia, Kuala Lumpur, Malaysia
2University of Western OntarioLondonOntarioN6A 5CI
3London Regional Cancer Research CentreLondonOntarioN6A 4L6Canada

Tóm tắt

AbstractThe tocotrienol‐rich fraction (TRF) of palm oil consists of tocotrienols and some α‐tocopherol (α‐T). Tocotrienols are a form of vitamin E having an unsaturated side‐chain, rather than the saturated side‐chain of the more common tocopherols. Because palm oil has been shown not to promote chemically‐induced mammary carcinogenesis, we tested effects of TRF and α‐T on the proliferation, growth, and plating efficiency (PE) of MDA‐MB‐435 estrogen‐receptor‐negative human breast cancer cells. TRF inhibited the proliferation of these cells with a concentration required to inhibit cell proliferation by 50% of 180 μg/mL, whereas α‐T had no effect at concentrations up to 1000 μg/mL as measured by incorporation of [3H]thymidine. The effects of TRF and α‐T also were tested in longer‐term growth experiments, using concentrations of 180 and 500 μg/mL. We found that TRF inhibited the growth of these cells by 50%, whereas α‐T did not. Their effect on the ability of these cells to form colonies also was studied, and it was found that TRF inhibited PE, whereas α‐T had no effect. These results suggest that the inhibition is due to the presence of tocotrienols in TRF rather than α‐T.

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