Effect of P stoichiometry on the abundance of nitrogen-cycle genes in phosphorus-limited paddy soil

Biology and Fertility of Soils - Tập 53 - Trang 767-776 - 2017
Xiaomeng Wei1,2, Yajun Hu1, Peiqin Peng3, Zhenke Zhu1, Cornelius Talade Atere1, Anthony G. O’Donnell2,4, Jinshui Wu1,2, Tida Ge1,2
1Key Laboratory of Agro-Ecological Processes in Subtropical Regions and Changsha Observation and Research Station for the Agricultural Environment, Chinese Academy of Sciences, Institute of Subtropical Agriculture, Changsha, China
2ISA-CAS and UWA Joint Laboratory for Soil Systems Biology, Changsha, China
3College of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha, China
4Institute of Agriculture, Faculty of Science, The University of Western Australia, Perth, Australia

Tóm tắt

Previous studies have shown that phosphorus addition to P-limited soils increases gaseous N loss. A possible explanation for this phenomenon is element stoichiometry (specifically of C:N:P) modifying linked nutrient cycling, leading to enhanced nitrification and denitrification. In this study, we investigated how P stoichiometry influenced the dynamics of soil N-cycle functional genes. Rice seedlings were planted in P-poor soils and incubated with or without P application. Quantitative PCR was then applied to analyze the abundance of ammonia-oxidizing (amoA) and denitrifying (narG nirK, nirS, nosZ) genes in soil. P addition reduced bacterial amoA abundance but increased denitrifying gene abundance. We suggest this outcome is due to P-induced shifts in soil C:P and N:P ratios that limited ammonia oxidization while enhancing P availability for denitrification. Under P application, the rhizosphere effect raised ammonia-oxidizing bacterial abundance (amoA gene) and reduced nirK, nirS, and nosZ in rhizosphere soils. The change likely occurred through greater C input and O2 release from roots, thus altering C availability and redox conditions for microbes. Our results show that P application enhances gaseous N loss potential in paddy fields mainly through stimulating denitrifier growth. We conclude that nutrient availability and elemental stoichiometry are important in regulating microbial gene responses, thereby influencing key ecosystem processes such as denitrification.

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Thông tin xuất bản

Biology and Fertility of Soils

Tập 53

767-776

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