Effect of 7β‐hydroxycholesterol on growth and membrane composition of Mycoplasma capricolum

FEBS Letters - Tập 232 - Trang 354-358 - 1988
I. Lelong, B. Luu1, M. Mersel2, S. Rottem
1Department of Membrane and Ultrastructure Research, The Hebrew University-Hadassah Medical School, Jerusalem 91010, Israel
2Centre deNeurochimie du CNRS, 67804 Strasbourg, France

Tóm tắt

7β‐OH cholesterol in a cholesterol rich growth medium (5–10 μg/ml) extended the lag period and slowed down the growth rate of Mycoplasma capricolum cells. In a cholesterol poor medium (0.5 μg/ml) inadequate to support growth, 7β‐OH cholesterol exerts a synergistic effect on growth. The 7β‐OH cholesterol was incorporated unchanged from the growth medium and could be recovered exclusively in the membrane fraction. The incorporation of the 7β‐OH cholesterol has no effect on the total phospholipid content but the DPG to PG ratio was markedly decreased. Exchange studies with lipid vesicles revealed that whereas most of the cholesterol underwent exchange, only about 20% of the 7β‐OH cholesterol was exchanged.

Tài liệu tham khảo

10.1016/0005-2760(75)90165-4 10.1016/S0021-9258(17)32730-8 10.1126/science.663671 10.1016/0006-291X(84)91306-8 10.1016/0006-291X(84)91432-3 10.1016/S0065-2911(08)60086-7 10.1016/0005-2736(80)90585-4 10.1016/0005-2736(73)90158-2 10.1021/bi00615a001 10.1073/pnas.75.9.4107 10.1021/bi00548a032 10.1073/pnas.80.3.692 1976 Chapman and Hall London S. Razin S. Rottem A.H. Maddy Biochemical Analysis of Membranes 3 26 10.1111/j.1432-1033.1982.tb05863.x Heitter H., 1988, J. Steroid Biochem. 10.1016/0003-9861(68)90637-1 10.1016/S0021-9258(19)52451-6 10.1139/y59-099 10.1016/0076-6879(66)08014-5 10.1016/0005-2736(81)90161-9 10.1128/jb.129.1.145-150.1977 10.1016/0968-0004(80)90074-2 10.1128/jb.167.3.986-991.1986 10.1016/S0022-2275(20)34423-0 10.1021/bi00607a005 10.1016/S0021-9258(17)43680-5 10.1021/bi00513a028 10.1016/0005-2736(86)90499-2
Thông tin
Thông tin xuất bản

FEBS Letters

Tập 232

354-358

Thông tin tác giả