EFFECTS AND SAFETY OF PLATELET-RICH PLASMA IN COMBINATION WITH HUMAN MESENCHYMAL STEM CELLS FROM AUTOLOGOUS ADIPOSE TISSUE FOR KNEE OSTEOARTHRITIS

Objective: To evaluate the efficacy of platelet-rich plasma in combination with human mesenchymal stem cells from autologous adipose tissue for knee osteoarthritis treatment. Research object and method: In the study, there are 30 patients including 26 females and 4 males; correspondingly, 60 knee joints were diagnosed with osteoarthritis according to the American College of Rheumatology (ACR) who are at the mean age of 58,63 ± 11.11, disease duration is from 5,3± 4,6 years which is respectively of stages II - III according to Kellgren and Lawrence. All were injected with autologous platelet-rich plasma that was extracted by PRP set, APC 30 PRP PRCEDURE PRAK and autologously extracted mesenchymal stem cells from abdominal adipose tissue using the ADI-25-01 ADIPOSEPRCEDURE PRAK set with the Harvest smart stem cell separator of TERUMO BCT- USA. Results: After 12 months of treatment: the pain level according to VAS score at the right knee joint was decreased from 6.0 ± 1.28 before treatment to 1.9 ± 0.3; VAS score at the left knee joint was decreased from 6.43±1.19 to 2.25±0.43. Total Liquesce score at right knee joint was decreased from 16.04±1.57 before treatment to 4.31±1.04, at left knee joint was decreased from 17.52±1.74 before treatment to 5.15. ±1.48. Total WOMAC score at right knee joint was decreased from 55.93±5.56 to 10.37±1.56; at left knee joint was decreased from 53.97±5.57 to 10.07±1.59. There were 52/60 (86.77%) joints with cartilage thickness change and the patellar cartilage thickness was increased from 1.56 ± 0.09 mm before treatment to 1.65 ± 0.09 mm. The rate of complications at injection site, abdominal adipose removal is low, there are no complications of local infection and systemic reactions. Conclusion: The treatment of knee osteoarthritis with platelet-rich plasma in combination with mesenchymal stem cells from autologous adipose tissue is effective in reducing pain, improving patient's mobility and walking function comparing with before treatment; reforming articular cartilage thickness on magnetic resonance image which therefore improves the mobility function of the knee joint. This therapy is safe.