Tăng cường MnSOD ty thể do EAE gây ra liên quan đến sự gia tăng protein SGK1 và Tom20 trong vỏ thận chuột

The Journal of Physiological Sciences - Tập 69 - Trang 723-732 - 2019
Sharanpreet Hira1, Balamuguran Packialakshmi1, Xiaoming Zhou1
1Department of Medicine, Uniformed Services University of the Health Sciences, Bethesda, USA

Tóm tắt

Sự chứng minh trước đây của chúng tôi rằng viêm tủy tự miễn dịch (EAE) nặng làm tăng độ phong phú protein MnSOD trong vỏ thận chuột đã dẫn đến nghiên cứu này nhằm làm rõ cơ chế tiềm ẩn với các tế bào HEK293 được xử lý bằng monensin như một mô hình. EAE nặng tăng cường độ phong phú protein ty thể của kinase SGK1 và Tom20, một tiểu đơn vị quan trọng của translocase ty thể trong vỏ thận. Trong các tế bào HEK293, catalase ức chế sự gia tăng protein SGK1 và Tom20 ty thể do monensin gây ra. Hơn nữa, GSK650394, một chất ức chế cụ thể của SGK1 làm giảm sự gia tăng độ phong phú protein ty thể của Tom20 và MnSOD do monensin gây ra. Cuối cùng, RNAi của Tom20 làm giảm tác động của monensin lên MnSOD. MnSOD và Tom20 liên kết vật lý với nhau. Tóm lại, trong các tế bào HEK293, các loại oxy phản ứng ty thể gia tăng độ phong phú protein của SGK1 ty thể, dẫn đến sự tăng lên của Tom20 ty thể, kết quả là nhập protein MnSOD vào ty thể. Đây có thể là một cơ chế mà qua đó EAE nặng kích thích tăng cường MnSOD ty thể trong vỏ thận.

Từ khóa

#EAE #MnSOD #SGK1 #Tom20 #ty thể #tế bào HEK293

Tài liệu tham khảo

Alvarez de la Rosa D, Coric T, Todorovic N, Shao D, Wang T, Canessa CM (2003) Distribution and regulation of expression of serum- and glucocorticoid-induced kinase-1 in the rat kidney. J Physiol 551:455–466 Cordas E, Naray-Fejes-Toth A, Fejes-Toth G (2007) Subcellular location of serum- and glucocorticoid-induced kinase-1 in renal and mammary epithelial cells. Am J Physiol Cell Physiol 292:C1971–1981 Dudek J, Rehling P, van der Laan M (2013) Mitochondrial protein import: common principles and physiological networks. Biochim Biophys Acta 1833:274–285 Efendiev R, Bertorello AM, Zandomeni R, Cinelli AR, Pedemonte CH (2002) Agonist-dependent regulation of renal Na+, K+-ATPase activity is modulated by intracellular sodium concentration. J Biol Chem 277:11489–11496 Engelsberg A, Kobelt F, Kuhl D (2006) The N-terminus of the serum- and glucocorticoid-inducible kinase Sgk1 specifies mitochondrial localization and rapid turnover. Biochem J 399:69–76 Ferraris JD, Williams CK, Persaud P, Zhang Z, Chen Y, Burg MB (2002) Activity of the TonEBP/OREBP transactivation domain varies directly with extracellular NaCl concentration. Proc Natl Acad Sci USA 99:739–744 Gerbeth C, Schmidt O, Rao S, Harbauer AB, Mikropoulou D, Opalinska M, Guiard B, Pfanner N, Meisinger C (2013) Glucose-induced regulation of protein import receptor Tom22 by cytosolic and mitochondria-bound kinases. Cell Metab 18:578–587 Grey JY, Connor MK, Gordon JW, Yano M, Mori M, Hood DA (2000) Tom20-mediated mitochondrial protein import in muscle cells during differentiation. Am J Physiol Cell Physiol 279:C1393–1400 Guffanti AA, Cohn DE, Kaback HR, Krulwich TA (1981) Relationship between the Na+/H+ antiporter and Na+/substrate symport in Bacillus alcalophilus. Proc Natl Acad Sci USA 78:1481–1484 Hucke S, Eschborn M, Liebmann M, Herold M, Freise N, Engbers A, Ehling P, Meuth SG, Roth J, Kuhlmann T, Wiendl H, Klotz L (2016) Sodium chloride promotes pro-inflammatory macrophage polarization thereby aggravating CNS autoimmunity. J Autoimmun 67:90–101 Ip WK, Medzhitov R (2015) Macrophages monitor tissue osmolarity and induce inflammatory response through NLRP3 and NLRC4 inflammasome activation. Nat Commun 6:6931 Iqbal S, Howard S, LoGrasso PV (2015) Serum- and glucocorticoid-inducible kinase 1 confers protection in cell-based and in in vivo neurotoxin models via the c-Jun N-terminal kinase signaling pathway. Mol Cell Biol 35:1992–2006 Jorg S, Kissel J, Manzel A, Kleinewietfeld M, Haghikia A, Gold R, Muller DN, Linker RA (2016) High salt drives Th17 responses in experimental autoimmune encephalomyelitis without impacting myeloid dendritic cells. Exp Neurol 279:212–222 Kaltimbacher V, Bonnet C, Lecoeuvre G, Forster V, Sahel JA, Corral-Debrinski M (2006) mRNA localization to the mitochondrial surface allows the efficient translocation inside the organelle of a nuclear recoded ATP6 protein. RNA 12:1408–1417 Kempson S, Thompson N, Pezzuto L, Glenn Bohlen H (2007) Nitric oxide production by mouse renal tubules can be increased by a sodium-dependent mechanism. Nitric Oxide 17:33–43 Kitada K, Nakano D, Liu Y, Fujisawa Y, Hitomi H, Shibayama Y, Shibata H, Nagai Y, Mori H, Masaki T, Kobori H, Nishiyama A (2012) Oxidative stress-induced glomerular mineralocorticoid receptor activation limits the benefit of salt reduction in Dahl salt-sensitive rats. PLoS One 7:e41896 Kleinewietfeld M, Manzel A, Titze J, Kvakan H, Yosef N, Linker RA, Muller DN, Hafler DA (2013) Sodium chloride drives autoimmune disease by the induction of pathogenic TH17 cells. Nature 496:518–522 Krementsov DN, Case LK, Hickey WF, Teuscher C (2015) Exacerbation of autoimmune neuroinflammation by dietary sodium is genetically controlled and sex specific. FASEB J 29:3446–3457 Lang F, Bohmer C, Palmada M, Seebohm G, Strutz-Seebohm N, Vallon V (2006) (Patho)physiological significance of the serum- and glucocorticoid-inducible kinase isoforms. Physiol Rev 86:1151–1178 Li Y, Huang TT, Carlson EJ, Melov S, Ursell PC, Olson JL, Noble LJ, Yoshimura MP, Berger C, Chan PH, Wallace DC, Epstein CJ (1995) Dilated cardiomyopathy and neonatal lethality in mutant mice lacking manganese superoxide dismutase. Nat Genet 11:376–381 Li Y, Reuter NP, Li X, Liu Q, Zhang J, Martin RC (2010) Colocalization of MnSOD expression in response to oxidative stress. Mol Carcinog 49:44–53 Luk E, Yang M, Jensen LT, Bourbonnais Y, Culotta VC (2005) Manganese activation of superoxide dismutase 2 in the mitochondria of Saccharomyces cerevisiae. J Biol Chem 280:22715–22720 Millar DG, Shore GC (1996) Signal anchor sequence insertion into the outer mitochondrial membrane. Comparison with porin and the matrix protein targeting pathway. J Biol Chem 271:25823–25829 Miranda PM, De Palma G, Serkis V, Lu J, Louis-Auguste MP, McCarville JL, Verdu EF, Collins SM, Bercik P (2018) High salt diet exacerbates colitis in mice by decreasing Lactobacillus levels and butyrate production. Microbiome 6:57 Packialakshmi B, Zhou X (2018) Experimental autoimmune encephalomyelitis (EAE) up-regulates the mitochondrial activity and manganese superoxide dismutase (MnSOD) in the mouse renal cortex. PLoS One 13:e0196277 Pao AC (2012) SGK regulation of renal sodium transport. Curr Opin Nephrol Hypertens 21:534–540 Sherk AB, Frigo DE, Schnackenberg CG, Bray JD, Laping NJ, Trizna W, Hammond M, Patterson JR, Thompson SK, Kazmin D, Norris JD, McDonnell DP (2008) Development of a small-molecule serum- and glucocorticoid-regulated kinase-1 antagonist and its evaluation as a prostate cancer therapeutic. Cancer Res 68:7475–7483 Sundstrom B, Johansson I, Rantapaa-Dahlqvist S (2015) Interaction between dietary sodium and smoking increases the risk for rheumatoid arthritis: results from a nested case-control study. Rheumatology (Oxford) 54:487–493 Wispe JR, Clark JC, Burhans MS, Kropp KE, Korfhagen TR, Whitsett JA (1989) Synthesis and processing of the precursor for human mangano-superoxide dismutase. Biochim Biophys Acta 994:30–36 Wu C, Yosef N, Thalhamer T, Zhu C, Xiao S, Kishi Y, Regev A, Kuchroo VK (2013) Induction of pathogenic TH17 cells by inducible salt-sensing kinase SGK1. Nature 496:513–517 Yamahara H, Kishimoto N, Nakata M, Okazaki A, Kimura T, Sonomura K, Matsuoka E, Shiotsu Y, Adachi T, Matsubara H, Iwasaka T, Mori Y (2009) Direct aldosterone action as a profibrotic factor via ROS-mediated SGK1 in peritoneal fibroblasts. Kidney Blood Press Res 32:185–193 Yang X, Yao G, Chen W, Tang X, Feng X, Sun L (2015) Exacerbation of lupus nephritis by high sodium chloride related to activation of SGK1 pathway. Int Immunopharmacol 29:568–573 Zhang Y, Baaden M, Yan J, Shao J, Qiu S, Wu Y, Ding Y (2010) The molecular recognition mechanism for superoxide dismutase presequence binding to the mitochondrial protein import receptor Tom20 from Oryza sativa involves an LRTLA motif. J Phys Chem B 114:13839–13846 Zhou X, Gallazzini M, Burg MB, Ferraris JD (2010) Contribution of SHP-1 protein tyrosine phosphatase to osmotic regulation of the transcription factor TonEBP/OREBP. Proc Natl Acad Sci USA 107:7072–7077 Zhou X, Packialakshmi B, Xiao Y, Nurmukhambetova S, Lees JR (2017) Progression of experimental autoimmune encephalomyelitis is associated with up-regulation of major sodium transporters in the mouse kidney cortex under a normal salt diet. Cell Immunol 317:18–25