Drug Effects on the Release of Endogenous Acetylcholine In Vivo: Measurement by Intracerebral Dialysis and Gas Chromatography–Mass Spectrometry

Journal of Neurochemistry - Tập 54 Số 6 - Trang 2016-2023 - 1990
Marc Marien1, Jean W. Richard1
1Department of Psychiatry, McGill University, Douglas Hospital Research Centre, Verdun, Quebec, Canada

Tóm tắt

Abstract: Intracerebral microdialysis was combined with a sensitive and specific gas chromatographic–mass spectrometric assay to measure the release of endogenous acetylcholine in the rat striatum in vivo. In rats anesthetized with urethane (1.2 g/kg i.p.), the levels of striatal acetylcholine dialyzed into a Ringer's perfusate were: (a) reliably measurable only in the presence of physostigmine; (b) stable at between 3 and 8 h of perfusion (30–75 pmol/20 min in the presence of 75 μM physostigmine); (c) reduced by calcium‐free Ringer's solution, tetrodotoxin (0.1 μM), and vesamicol (1.0 μM); and (d) increased by elevated potassium (100 mM), atropine (3–300 μM), and haloperidol (0.75 mg/kg i.p.). In conscious unrestrained rats, the spontaneous release of striatal acetylcholine was not altered significantly following the administration of urethane. The changes in acetylcholine release observed in this study are consistent with the known actions of some drugs or ionic conditions on striatal cholinergic neurotransmission and are evident under the condition of urethane anesthesia. The present results demonstrate the sensitivity and suitability of this method for monitoring endogenous striatal acetylcholine release in vivo.

Từ khóa


Tài liệu tham khảo

10.1016/0304-3940(87)90352-1

AlberyW. J. BoutelleM. CassA. E. G. DurrantS. L. T. FillenzM. andZetterstromT.(1987)A new assay for measuring basal acetylcholine levels in rat brain dialysate.Proc. Br. Pharmacol. Soc.(9–11 Sept. 1987 Oxford) abstract C133.

Anderson D. C., 1983, Pharmacological characterization of the acetylcholine transport system in purified Torpedo electric organ synaptic vesicles, Mol. Pharmacol., 24, 48

10.1111/j.1471-4159.1985.tb05421.x

10.1111/j.1471-4159.1966.tb04265.x

Cheney D., 1989, Neuromethods. Vol. 12: Neurotransmitter Turnover, 443

10.1111/j.1471-4159.1987.tb05686.x

Consolo S., 1987, Cellular and Molecular Basis of Cholinergic Function, 256

10.1111/j.1471-4159.1983.tb11818.x

Cubeddu L. X., 1983, Frequency‐dependent effects of neuronal uptake inhibitors on the autoreceptor‐mediated modulation of dopamine and acetylcholine release from the rabbit striatum, J. Pharmacol. Exp. Ther., 226, 88

DamsmaG.(1987)Microdialysis of acetylcholine from the rat brain: analytical methodological and pharmacological aspects.Doctoral thesis Medicinal Chemistry Group Pharmaceutical Laboratories University of Groningen pp.28–30.

10.1111/j.1471-4159.1985.tb07238.x

10.1111/j.1471-4159.1987.tb05695.x

10.1016/0024-3205(87)90184-6

Delgado J. M. R., 1972, Dialytrode for long term intracerebral perfusion in awake monkeys, Arch. Int. Pharmacodyn. Ther., 198, 9

10.1111/j.1749-6632.1986.tb23600.x

Gaddum J. H., 1961, Push pull cannulae, J. Physiol. (Lond.), 155, 1P

Gaddum J. H., 1962, Pharmacological Analysis of Central Nervous Action, 1

10.1111/j.1471-4159.1973.tb12097.x

10.1016/0006-8993(77)90482-6

10.1038/220915a0

10.1007/BF00499253

10.1111/j.1471-4159.1982.tb04727.x

Jenden D. J., 1974, Choline and Acetylcholine: Handbook of Chemical Assay Methods, 135

10.1016/0003-2697(73)90134-6

Korf J., 1986, Monitoring Neurotransmitter Release During Behaviour, 182

10.1016/0306-4522(83)90102-1

MacIntoshF. C.andOborinP. E.(1953)Release of acetylcholine from intact cerebral cortex.Abstracts of the XIX International Physiology Congress pp.580–581.

10.1007/BF01952426

10.1016/0165-6147(87)90078-2

10.1113/jphysiol.1963.sp007045

Moroni F., 1984, Measurement of Neurotransmitter Release In Vivo, 63

10.1038/182317a0

10.1111/j.1749-6632.1986.tb23601.x

Paxinos G., 1986, The Rat Brain in Stereotoxic Coordinates

10.1111/j.1471-4159.1983.tb13668.x

Racagni G., 1976, In vivo actions of clozapine and haloperidol on the turnover rate of acetylcholine in rat striatum, J. Pharmacol. Exp. Ther., 196, 323

10.1016/0006-8993(89)90826-3

Scatton B., 1982, Effect of dopamine agonists and neuroleptic agents on striatal acetylcholine transmission in the rat: evidence against dopamine receptor multiplicity, J. Pharmacol. Exp. Ther., 220, 197

10.1111/j.1471-4159.1967.tb09886.x

Stavinoha W. B., 1965, Estimation of the acetylcholine content in rat brain by gas chromatography, J. Pharmacol. Exp. Ther., 150, 231

10.1016/0378-4347(87)80330-4

Ungerstedt U., 1984, Measurement of Neurotransmitter Release In Vivo, 81

10.1111/j.1476-5381.1969.tb10570.x

10.1139/y79-061

10.1016/0028-3908(79)90094-7

Thông tin
Thông tin xuất bản

Journal of Neurochemistry

Tập 54 Số 6

2016-2023

Thông tin tác giả