Phân bố và đa dạng của nấm mycorrhiza dạng sợi ở cây nho từ những vườn sản xuất dọc theo bờ biển Adriatic phía đông

Mycorrhiza - Tập 23 Số 3 - Trang 209-219 - 2013
Likar, Matevž1, Hančević, Katarina2, Radić, Tomislav2, Regvar, Marjana1
1Department of Biology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
2Institute for Adriatic Crops and Karst Reclamation, Split, Croatia

Tóm tắt

Sự định cư và đa dạng của nấm mycorrhiza dạng sợi (AMF) trên rễ của cây nho đã được nghiên cứu tại các vườn nho sản xuất nằm dọc theo một đoạn karst dài 500 km bên bờ biển Adriatic. Cộng đồng AMF trên rễ cây nho đã được phân tích bằng phương pháp điện di gel nhiệt độ tạm thời và giải trình tự các đoạn 18S và đoạn không chuyển đoạn nội bộ của operon rDNA. Sự định cư của AMF trên các rễ cây nho này có sự nhất quán suốt toàn bộ khu vực karst Adriatic phía đông, đạt từ 64 đến 82% rễ con. So sánh các cộng đồng AMF trên rễ cây nho cho thấy cộng đồng nấm liên quan đến rễ cây nho có vẻ ổn định tương đối, với sự biến đổi giữa các vườn rượu so với sự biến đổi trong cùng một vườn khá tương đương. Một số thay đổi trong các cộng đồng nấm được quy cho các yếu tố môi trường (P có sẵn cho cây) và vị trí của vườn nho, mặc dù cái sau cũng có thể bị ảnh hưởng bởi một yếu tố môi trường khác chưa được đo lường. Tổng cộng có 27 loại nấm được xác định, bao gồm các loại từ nhóm Glomus B, dựa trên giải trình tự 18S rDNA. Giải trình tự của đoạn không chuyển đoạn nội bộ rDNA đã cho ra 30 loại nấm khác nhau, trong đó có tám loại Glomeromycota khác nhau, bao gồm Glomus sinuosum và Glomus indicum. Theo những gì chúng tôi biết, đây là báo cáo đầu tiên về sự định cư của cây nho bởi G. indicum.

Từ khóa

#nấm mycorrhiza dạng sợi #cây nho #vườn nho #Adriatic #Glomus indicum

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