Disruption of TRI101 , the Gene Encoding Trichothecene 3- O -Acetyltransferase, from Fusarium sporotrichioides

Applied and Environmental Microbiology - Tập 65 Số 12 - Trang 5252-5256 - 1999
Susan P. McCormick1, Nancy J. Alexander1, Susan E. Trapp2, Thomas Höhn1
1Mycotoxin Research Unit, USDA/ARS National Center for Agricultural Utilization Research, Peoria, Illinois 61604,1 and
2Department of Chemistry, University of Maryland, College Park, Maryland 207422

Tóm tắt

ABSTRACT We screened a Fusarium sporotrichioides NRRL 3299 cDNA expression library in a toxin-sensitive Saccharomyces cerevisiae strain lacking a functional PDR5 gene. Fourteen yeast transformants were identified as resistant to the trichothecene 4,15-diacetoxyscirpenol, and each carried a cDNA encoding the trichothecene 3- O -acetyltransferase that is the F. sporotrichioides homolog of the Fusarium graminearum TRI101 gene. Mutants of F. sporotrichioides NRRL 3299 produced by disruption of TRI101 were altered in their abilities to synthesize T-2 toxin and accumulated isotrichodermol and small amounts of 3,15-didecalonectrin and 3-decalonectrin, trichothecenes that are not observed in cultures of the parent strain. Our results indicate that TRI101 converts isotrichodermol to isotrichodermin and is required for the biosynthesis of T-2 toxin.

Từ khóa


Tài liệu tham khảo

10.1128/AEM.64.1.221-225.1998

10.1007/s004380051046

10.1146/annurev.mi.43.100189.001231

10.1094/MPMI.1997.10.2.147

10.1128/mr.57.3.595-604.1993

10.1094/MPMI-9-0775

10.1093/nar/20.6.1425

10.1016/0378-1119(88)90533-1

10.1007/BF02456618

10.1006/fgbi.1999.1122

Hohn T. M. McCormick S. P. Alexander N. J. Desjardins A. E. Proctor R. H. Function and biosynthesis of trichothecenes produced by Fusarium species Molecular genetics of host-specific toxins in plant disease. Kohmoto K. Yoder O. C. 1998 17 24 Kluwer Academic Publishers Dordrecht The Netherlands

10.1007/BF00336778

10.1074/jbc.273.3.1654

10.1016/S0014-5793(98)01061-8

10.1271/bbb.62.1033

10.1016/0378-1119(86)90364-1

10.1007/BF00336747

10.1128/aem.63.5.1685-1688.1997

10.1128/aem.62.2.353-359.1996

10.1128/aem.56.3.702-706.1990

McLaughlin C. S. Vaughn M. H. Campbell J. M. Wei C. M. Stafford M. E. Inhibition of protein synthesis by trichothecenes Mycotoxins in human and animal health. Rodricks J. V. Hesseltine C. W. Mehlman M. A. 1977 263 273 Pathotoxin Publishers Park Forest Ill

10.1007/BF00351651

10.1080/09540109609354902

10.1094/MPMI-8-0593

10.1128/aem.61.5.1923-1930.1995

Sambrook J. Fritsch E. F. Maniatis T. Molecular cloning: a laboratory manual 2nd ed. 1989 Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y

10.1007/BF00340712

10.1128/aem.63.10.3825-3830.1997

Stevens R. R. Mycology guidebook 1974 703 University of Washington Press Seattle

10.1046/j.1365-313x.1998.00174.x

10.1007/s004380050666

10.1128/mcb.7.9.3297-3305.1987

10.1128/am.30.1.4-9.1975

Thông tin
Thông tin xuất bản

Applied and Environmental Microbiology

Tập 65 Số 12

5252-5256

Thông tin tác giả