Development of genomic‐derived simple sequence repeat markers in Hevea brasiliensis from 454 genome shotgun sequences

Plant Breeding - Tập 131 Số 4 - Trang 555-562 - 2012
Wirulda Pootakham1, Juntima Chanprasert1, Nukoon Jomchai1, Duangjai Sangsrakru1, Thippawan Yoocha1, Somvong Tragoonrung1, Sithichoke Tangphatsornruang2
1; National Center for Genetic Engineering and Biotechnology, 113 Phaholyothin Rd., Klong 1, Klong Luang, Pathumthani 12120, Thailand
2Corresponding author, E‐mail: [email protected]

Tóm tắt

With 2 figures and 2 tablesAbstractIn an attempt to develop additional microsatellite markers in rubber tree, we employed a 454 pyrosequencing technology to obtain genomic shotgun sequences and subsequently identified over 24 000 putative simple sequence repeats (SSRs). A total of 418 potential SSR loci were chosen for an empirical validation. Two hundred and twenty‐four primer pairs yielded successful amplification, and 90 SSR markers exhibited polymorphism among the 18 rubber tree accessions evaluated. The number of alleles per locus ranged from 2 to 7, with an average of 3.44 alleles per SSR. The gene diversity of individual microsatellite loci displayed a broad range of values from 0.104 to 0.901 with a mean of 0.612. The polymorphism information content also varied greatly from 0.099 to 0.893 with an average of 0.577. We also identified a set of five highly informative markers that were able to unequivocally distinguish 18 rubber tree genotypes examined in this study.

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Thông tin
Thông tin xuất bản

Plant Breeding

Tập 131 Số 4

555-562

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