Nội dung được dịch bởi AI, chỉ mang tính chất tham khảo
Phát triển và ứng dụng các dấu hiệu dựa trên SINE cho việc phân loại giống khoai tây
Tóm tắt
Việc phân loại giống khoai tây dựa trên các đặc điểm hình thái rất tốn công sức và bị ảnh hưởng bởi môi trường, trong khi các dấu hiệu phân tử hiện tại thường tốn kém hoặc mất nhiều thời gian để phát triển hoặc áp dụng. SINEs, hay các yếu tố hạt nhân ngắt quãng ngắn, là những retrotransposon có số lượng sao chép cao trong bộ gen thực vật, đại diện cho một nguồn tiềm năng cho các dấu hiệu mới. Chúng tôi đã phát triển một hệ thống dấu hiệu cho phân loại giống khoai tây, được gọi là đa hình khuếch đại giữa SINE (ISAP). Dựa trên chín họ SINE khoai tây mới được đặc trưng (Wenke et al. trong Plant Cell 23:3117–3128, 2011), chúng tôi đã thiết kế các mồi SINE đặc hiệu cho loài. Từ 153 tổ hợp mồi thu được, chúng tôi đã chọn ra các bộ mồi thông tin cao cho phân tích giống khoai tây dựa trên số lượng băng, chất lượng mẫu băng và mức độ đa hình. Các đoạn đại diện cho ISAP có thể được tách biệt bằng điện di gel agarose thông thường; tuy nhiên, tự động hóa bằng máy giải trình tự mao quản là khả thi. Hai họ SINE được chọn, SolS-IIIa và SolS-IV, đã được chứng minh là khuếch đại cao nhưng khác nhau trong họ Solanaceae, bộ tộc Solaneae, bao gồm khoai tây hoang dã và trồng trọt, cà chua và cà tím. Kỹ thuật lai phân tử huỳnh quang cho thấy sự phân bố genome rộng rãi của SolS-IIIa và SolS-IV dọc theo các nhiễm sắc thể khoai tây, tạo cơ sở cho việc phân loại và phân biệt kiểu gen của các biến thể somaclonal bằng dấu hiệu ISAP.
Từ khóa
#khoai tây #dấu hiệu phân tử #SINE #đa hình khuếch đại giữa SINE #ISAP #phân tích giốngTài liệu tham khảo
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