Detection of Multiple Noroviruses Associated with an International Gastroenteritis Outbreak Linked to Oyster Consumption

Journal of Clinical Microbiology - Tập 44 Số 11 - Trang 3878-3882 - 2006
Soizick Le Guyader1, Fabienne Bon2, Dario De Medici3, Sylvain Parnaudeau1, Alessandra Bertone4, Silvia Crudeli5, Aoife M. Doyle6, Mohamed Zidane1, Pier Sandro Cocconcelli3, Évelyne Kohli2, Francesco Maddalo4, Marina Monini5, Anne Gallay6, M. Pommepuy1, Pierre Pothier2, Maria Chironna5
1Laboratoire de Microbiologie, Institut Français pour la Recherche et l'Exploitation de la Mer, Nantes, France
2Laboratoire de Virologie et Microbiologie Médicale et Moléculaire, Centre Hospitalier Universitaire, Dijon, France
3Centro Nazionale per la Qualità degli Alimenti e per i Rischi Alimentari, Rome, Italy
4Azienda Unità Sanitaria Locale, La Spezia, Italy
5Dipartimento di Sanità alimentare e animale, Istituto Superiore di Sanità, Rome, Italy
6Institut de Veille Sanitaire, Département Maladies Infectieuses, Unité Infections Entériques, Alimentaires et Zoonoses, Saint-Maurice, France

Tóm tắt

ABSTRACT An international outbreak linked to oyster consumption involving a group of over 200 people in Italy and 127 total subjects in 13 smaller clusters in France was analyzed using epidemiological and clinical data and shellfish samples. Environmental information from the oyster-producing area, located in a lagoon in southern France, was collected to investigate the possible events leading to the contamination. Virologic analyses were conducted by reverse transcription-PCR (RT-PCR) using the same primer sets for both clinical and environmental samples. After sequencing, the data were analyzed through the database operated by the scientific network FoodBorne Viruses in Europe. The existence of an international collaboration between laboratories was critical to rapidly connect the data and to fully interpret the results, since it was not obvious that one food could be the link because of the diversity of the several norovirus strains involved in the different cases. It was also demonstrated that heavy rain was responsible for the accidental contamination of seafood, leading to a concentration of up to hundreds of genomic copies per oyster as detected by real-time RT-PCR.

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Thông tin xuất bản

Journal of Clinical Microbiology

Tập 44 Số 11

3878-3882

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