DNA strand breakage in aquatic organisms and the potential value of the comet assay in environmental monitoring

Carys L. Mitchelmore1, James Kevin Chipman1
1School of Biochemistry, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK

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J.A. Miller, E.C. Miller, Ultimate chemical carcinogens as reactive mutagenic electrophiles. In: H.H. Hiatt, J.D. Watson, J.A. Winsten (Eds.), Origins of Human Cancer, Cold Spring Harbor Laboratory, 1977, pp. 605–628.

Weinberg, 1989, Oncogenes, antioncogenes and the molecular bases of multistep carcinogenesis, Cancer Res., 49, 3713

Bailey, 1996, Fish models for environmental carcinogenesis in the rainbow trout, Environ. Health Perspect., 104, 5, 10.1289/ehp.96104s15

Kurelec, 1993, The genotoxic disease syndrome, Mar. Environ. Res., 35, 341, 10.1016/0141-1136(93)90101-5

De Flora, 1991, Genotoxic, carcinogenic, and teratogenic hazards in the marine environment, with special reference to the Mediterranean Sea, Mutat. Res., 258, 285, 10.1016/0165-1110(91)90013-L

Wurgler, 1992, Environmental effects of genotoxins (eco-genotoxology), Mutagenesis, 7, 321, 10.1093/mutage/7.5.321

Richter, 1988, Normal oxidative damage to mitochondrial and nuclear DNA is extensive, Proc. Natl. Acad. Sci. U.S.A., 85, 6465, 10.1073/pnas.85.17.6465

Mitchelmore, 1996, Normal status of hepatic 7-ethoxyresorufin O-deethylase (EROD) activity, antioxidant enzymes and DNA oxidation in turbot (Scophthalamus maximus) and other flatfish species following exposure to nitroaromatic compounds, Mar. Environ. Res., 42, 329, 10.1016/0141-1136(95)00073-9

Demple, 1994, Repair of oxidative damage to DNA: enzymology and biology, Annu. Rev. Biochem., 63, 915, 10.1146/annurev.bi.63.070194.004411

Epe, 1995, DNA damage profiles induced by oxidising agents, Rev. Physiol. Biochem. Pharmacol., 27, 223, 10.1007/BFb0048268

Lemaire, 1994, Stimulation of oxyradical production of hepatic microsomes of flounder (Platichthys flesus) and perch (Perca fluviatilis) by model and pollutant xenobiotics, Arch. Environ. Contam. Toxicol., 26, 191, 10.1007/BF00224804

Garcia Martinez, 1995, Benzo(a)pyrene-dione-stimulated oxyradical production by microsomes of digestive gland of the common mussel, Mytilus edulis L., Mar. Environ. Res., 39, 185, 10.1016/0141-1136(94)00032-K

Garcia Martinez, 1995, Nitrofurantoin-stimulated reactive oxygen species production and genotoxicity in digestive gland microsomes and cytosol of the common mussel (Mytilus edulis L.), Toxicol. Appl. Pharmacol., 131, 332, 10.1006/taap.1995.1076

Lemaire, 1993, Pro-oxidant/antioxidant processes and organic xenobiotic interactions in marine organisms, in particular the flounder Platichthys flesus and the mussel Mytilus edulis, Trends Comp. Biochem. Physiol., 1, 1119

Winston, 1991, Prooxidant and antioxidant mechanisms in aquatic organisms, Aquat. Toxicol., 19, 137, 10.1016/0166-445X(91)90033-6

Chaney, 1996, DNA repair: enzymatic mechanisms and relevance to drug response, J. Natl. Cancer Inst., 88, 1346, 10.1093/jnci/88.19.1346

George, 1990, Activities of pollutant metabolising and detoxication systems in the liver of plaice, Pleuronectes platessa: sex and seasonal variations in non-induced fish, Comp. Biochem. Physiol. C Pharmacol. Toxicol. Endocrinol., 96, 185, 10.1016/0742-8413(90)90066-I

J.J. Stegeman, M. Brouwer, R.T. DiGiulio, L. Förlin, B. Fowler, B.M. Sanders, P.A. Van Veld, Molecular responses to environmental contaminants: enzyme and protein synthesis as indicators of chemical exposure and effect, in: R.J. Huggett, R.A. Kimerle, P.M. Mehrle Jr., H.L. Bergman (Eds.), Biomarkers, Biochemical, Physiological, and Histological Markers of Anthropogenic Stress, Lewis Pub., Boca Raton, FL, 1992, pp. 235–335.

S.G. George, Enzymology and molecular biology of Phase II xenobiotic-conjugating enzymes in fish, in: D.C. Malins, G.K. Ostrander (Eds.), Aquatic Toxicology, Molecular, Biochemical and Cellular Perspectives, CRC Press, Boca Raton, FL, 1994, pp. 37–86.

D.R. Livingstone, Cytochrome P450 in pollution monitoring. Use of cytochrome P4501A (CYP1A) as a biomarker of organic pollution in aquatic and other organisms, in: M. Richardson (Ed.), Environmental Xenobiotics, Taylor and Francis, London, 1996, pp. 143–160.

Poirier, 1993, Antisera specific for carcinogen DNA adducts and carcinogen modified DNA: applications for detection of xenobiotics in biological samples, Mutat. Res., 288, 31, 10.1016/0027-5107(93)90205-T

Randerath, 1994, 32P-postlabelling methods for DNA adduct detection: overview and critical evaluation, Drug Metab. Rev., 26, 67, 10.3109/03602539409029785

A.E. Maccubbin, DNA adduct analysis in fish: laboratory and field studies, in: D.C. Malins, G.K. Ostrander (Eds.), Aquatic Toxicology: Molecular, Biochemical and Cellular Perspectives, Lewis Publishers, Boca Raton, FL, 1994, pp. 267–294.

L. Shugart, Biological monitoring: testing for genotoxicity, in: J.F. McCarthy, L.R. Shugart (Eds.), Biomarkers of Environmental Contamination, Lewis Publishers, Chelsea, 1990, pp. 217–227.

Sina, 1983, Evaluation of the alkaline elution/rat hepatocyte assay as a predictor of carcinogenic/mutagenic potential, Mutat. Res., 113, 357, 10.1016/0165-1161(83)90228-5

Eastman, 1992, The origins of DNA breaks: a consequence of DNA damage, DNA repair or apoptosis?, Cancer Invest., 10, 229, 10.3109/07357909209032765

T. Lindahl, T. Ljungquist, Apurimic and apurimidinic sites in DNA, in: P.C. Hanawalt, R.B. Setlow (Eds.), Molecular Mechanisms for Repair of DNA, Part A, Plenum, New York, 1975, pp. 31–38.

Park, 1991, Accumulation of polycyclic aromatic hydrocarbon-induced single-strand breaks is attributed to slower rejoining processes by DNA polymerase inhibitor, cytosine arabinoside in CHO-K1 cells, Life Sci., 48, 1255, 10.1016/0024-3205(91)90520-L

Speit, 1995, The contribution of excision repair to the DNA effects seen in the alkaline single cell gel test (comet assay), Mutagenesis, 10, 555, 10.1093/mutage/10.6.555

Pandrangi, 1995, Alkaline single cell gel (Comet) assay and genotoxicity monitoring using bullheads and carp, Environ. Mol. Mutag., 26, 345, 10.1002/em.2850260411

Koppen, 1996, The alkaline comet test on plant cells: a new genotoxicity test for DNA strand breaks in Vicia faba root cells, Mutat. Res., 360, 193, 10.1016/S0165-1161(96)90017-5

Leroy, 1996, Evaluation of three methods for the detection of DNA single-strand breaks in human lymphocytes: alkaline elution, nick translation, and single cell gel electrophoresis, J. Toxicol. Environ. Health, 47, 409, 10.1080/009841096161573

McConkey, 1988, Stimulation of endogenous endonuclease activity in hepatocytes exposed to oxidative stress, Toxicol. Lett., 42, 123, 10.1016/0378-4274(88)90069-0

Meneghini, 1988, Genotoxicity of active oxygen species in mammalian cells, Mutat. Res., 195, 215, 10.1016/0165-1110(88)90001-2

Hartmann, 1997, The contribution of cytotoxicity to DNA—effects in the single cell gel test (comet assay), Toxicol. Lett., 90, 183, 10.1016/S0378-4274(96)03847-7

J.T. Lett, Measurement of DNA single-strand breaks by sedimentation in alkaline sucrose gradients, in: E.C. Freidberg, Hanawalt, P.C. (Eds.), DNA Repair: A Laboratory Manual in Research Procedures, Marcel Dekker, 1981, pp. 403–418.

Ahnstrom, 1988, Techniques to measure single strand breaks in cells. A review, Int. J. Radiat. Biol., 54, 695, 10.1080/09553008814552151

Cook, 1978, Spectrofluorometric measurements of the binding of ethidium to superhelical DNA from cell nuclei, Eur. J. Biochem., 84, 465, 10.1111/j.1432-1033.1978.tb12188.x

Blakely, 1982, A quantitative assay of deoxyguanosine strand breaks and their repair in mammalian cells, Anal. Biochem., 124, 125, 10.1016/0003-2697(82)90229-9

Birnboim, 1981, Fluorometric method for rapid detection of DNA strand breaks in human white blood cells produced by low doses of radiation, Cancer Res., 41, 1889

Ahnstrom, 1973, Radiation induced strand breakage in DNA from mammalian cells. Strand separation in alkaline solution, Radiat. Biol., 23, 285

Parodi, 1989, DNA damage in mouse and rat liver by caprolactam and benzoin, evaluated with three different methods, Mutat. Res., 224, 379, 10.1016/0165-1218(89)90185-7

Singh, 1988, A simple technique for quantitation of low levels of DNA damage in individual cells, Exp. Cell Res., 237, 123

McKelvey-Martin, 1993, The single cell gel electrophoresis assay (comet assay): a European review, Mutat. Res., 288, 47, 10.1016/0027-5107(93)90207-V

Fairbairn, 1995, The comet assay: a comprehensive review, Mutat. Res., 339, 37, 10.1016/0165-1110(94)00013-3

Collins, 1992, Workshop on single-cell gel electrophoresis (the `comet' assay) held as part of the UKEMS/DNA repair network joint meeting, Swansea, March 1992, Mutagenesis, 7, 357, 10.1093/mutage/7.5.357

Anderson, 1994, The effects of various antioxidants and other modifying agents on oxygen–radical-generated DNA damage in human lymphocytes in the COMET assay, Mutat. Res., 307, 261, 10.1016/0027-5107(94)90300-X

C.L. Mitchelmore, J.K. Chipman, Detection of DNA strand breaks in brown trout (Salmo trutta) hepatocytes and blood cells using the single cell gel electrophoresis (comet) assay. Aquat. Toxicol (1998) in press.

Mitchelmore, 1997, Polycyclic and nitro-aromatic compounds produce DNA strand breakage in Brown trout (Salmo trutta) and mussel (Mytilus edulis L.) cells, Mutagenesis, 12, 101

C.L. Mitchelmore, C. Birmelin, D.R. Livingstone, J.K. Chipman, The detection of DNA strand breaks in isolated mussel (Mytilus edulis L.) digestive gland cells using the `comet' assay, Ecotoxicol. Environ. Safety (1998) in press.

C.L. Mitchelmore, C. Birmelin, D.R. Livingstone, J.K. Chipman, Evidence for cytochrome-P450 catalysis and free radical involvement in the production of DNA strand breaks by benzo[a]pyrene and nitroaromatics in mussel (Mytilus edulis L.) digestive gland cells, Aquat. Toxicol. (1998) in press.

Gedik, 1992, Single-cell gel electrophoresis applied to the analysis of UV–C damage and its repair in human cells, Int. J. Radiat. Biol., 62, 313, 10.1080/09553009214552161

C. Birmelin, C.L. Mitchelmore, P.S. Goldfarb, D.R. Livingstone. Characterisation of biotransformation enzyme activities and DNA integrity in isolated cells of digestive gland of the common mussel, Mytilus edulis L., Comp. Biochem. Physiol., 1998, in press.

Singh, 1993, A microgel electrophoresis technique for the direct quantitation of DNA damage and repair in individual fibroblasts cultured on microscope slides, Mutat. Res., 252, 289, 10.1016/0165-1161(91)90008-V

Bihari, 1992, Fractionation of DNA from marine invertebrate (Maja crispata, Mytilus galloprovincialis) haemolymph by alkaline elution, Comp. Biochem. Physiol. B Biochem. Mol. Biol., 102, 419, 10.1016/0305-0491(92)90145-H

Nacci, 1992, Application of the DNA alkaline unwinding assay to detect DNA strand breaks in marine bivalves, Mar. Environ. Res., 33, 83, 10.1016/0141-1136(92)90134-8

Singh, 1989, Abundant alkali sensitive sites in DNA of human and mouse sperm, Exp. Cell Res., 184, 461, 10.1016/0014-4827(89)90344-3

Liepelt, 1995, Induction of DNA strand breaks in rainbow trout Oncorhynchus mykiss under hypoxic and hyperoxic conditions, Aquat. Toxicol., 33, 177, 10.1016/0166-445X(95)00016-W

Shugart, 1988, Quantitation of chemically induced damage to DNA of aquatic organisms by alkaline unwinding assay, Aquat. Toxicol., 13, 43, 10.1016/0166-445X(88)90071-9

Walton, 1984, DNA repair synthesis following exposure to chemical mutagens in primary liver, stomach and intestinal cells isolated from Rainbow trout, Cancer Res., 44, 1120

Espina, 1995, DNA-repair in fish from polluted estuaries, Mar. Environ. Res., 39, 309, 10.1016/0141-1136(94)00025-K

Steinert, 1996, Contribution of apoptosis to observed DNA damage in mussel cells, Mar. Environ. Res., 42, 253, 10.1016/0141-1136(95)00056-9

Olive, 1993, Radiation-induced apoptosis measured in TK6 human B lymphoblast cells using the comet assay, Radiat. Res., 136, 130, 10.2307/3578650

Anderson, 1996, An investigation of bone marrow and testicular cells in vivo using the comet assay, Mutat. Res., 370, 159, 10.1016/S0165-1218(96)00061-4

Gagni, 1995, Evaluation of the genotoxicity of environmental contaminants in sediments to rainbow trout hepatocytes, Environ. Toxicol. Water Qual. Int. J., 10, 217, 10.1002/tox.2530100309

Nacci, 1996, Detection of DNA damage in individual cells from marine organisms using the single cell gel assay, Aquat. Toxicol., 35, 197, 10.1016/0166-445X(96)00016-1

Herbert, 1990, Monitoring DNA damage in Mytilus galloprovincialis and other aquatic animals, Z. Angew. Zool., 77, 13

Betti, 1996, The comet assay for the evaluation of the genetic hazard of pollutants in cetaceans—preliminary results on the genotoxic effects of methyl mercury on the bottle nosed dolphin (Tursiops truncatus) lymphocytes in vitro, Mar. Pollut. Bull., 32, 545, 10.1016/0025-326X(96)84573-5

Black, 1996, DNA strand breakage in freshwater mussels (Anodonta grandis) exposed to lead in the laboratory and field, Environ. Toxicol. Chem., 15, 802, 10.1002/etc.5620150528

Batel, 1993, Nonradiometric detection of DNA crosslinks in mussel hemolymph by alkaline elution, Anal. Biochem., 212, 402, 10.1006/abio.1993.1347

Ralph, 1996, Alkaline single cell gel (comet) assay and genotoxicity monitoring using two species of tadpoles, Environ. Mol. Mutag., 28, 112, 10.1002/(SICI)1098-2280(1996)28:2<112::AID-EM7>3.0.CO;2-H

Accomando, 1991, A rapid method for detecting DNA strand breaks in Mytilus galloprovincialis Lam. induced by genotoxic xenobiotic chemicals, Int. J. Biochem., 23, 227, 10.1016/0020-711X(91)90194-R

Bolognesi, 1996, Genotoxicity biomarkers in Mytilus galloprovincialis as indicators of marine pollutants, Comp. Biochem. Physiol. C Pharmacol. Toxicol. Endocrinol., 113, 319, 10.1016/0742-8413(95)02103-5

Belpaeme, 1996, Cytogenetic studies of PCB77 on brown trout (Salmo trutta fario) using the micronucleus test and the alkaline comet assay, Mutagenesis, 11, 485, 10.1093/mutage/11.5.485

Bihari, 1990, DNA damage determination by the alkaline elution technique in the haemolymph of mussel Mytilus galloprovincialis treated with benzo[a]pyrene and 4-nitroquinoline-N-oxide, Aquat. Toxicol., 18, 13, 10.1016/0166-445X(90)90032-K

Shugart, 1988, An alkaline unwinding assay for the detection of DNA damage in aquatic organisms, Mar. Environ. Res., 24, 321, 10.1016/0141-1136(88)90329-7

N. Bihari, R. Batel, R.K. Zahn, The use of alkaline elution procedure to measure DNA damage in crab haemolymph treated with benzo[a]pyrene, in: Carcinogenic, Mutagenic, Teratogenic Marine Pollutants: Impact on Human Health and the Environment, Adv. Appl. Biotechnol. Ser., Vol. 5., Gulf Pub., TX, USA, 1990, pp. 121–127.

Theodorakis, 1992, Sequential expression of biomarkers in bluegill sunfish exposed to contaminated sediment, Ecotoxicology, 1, 45, 10.1007/BF00702655

Theodorakis, 1994, Detection of genotoxic insult as DNA strand breaks in fish blood cells by agarose gel electrophoresis, Environ. Toxicol. Chem., 13, 1023, 10.1002/etc.5620130703

Batel, 1985, DNA damage by benzo[a]pyrene in the liver of mosquito fish Gambusia affinis, Sci. Total Environ., 41, 275, 10.1016/0048-9697(85)90146-9

Di Giulio, 1993, Effects of Black Rock harbour sediments on indexes of biotransformation, oxidative stress, and DNA integrity in channel catfish, Aquat. Toxicol., 26, 1, 10.1016/0166-445X(93)90002-I

Waldmann, 1995, Increased genotoxicity of acetylaminofluorene by modulators of multixenobiotic resistance mechanism: studies with the fresh water clam Corbicula fluminea, Mutat. Res., 342, 113, 10.1016/0165-1218(95)90021-7

Hasspieler, 1996, Influence of DT diaphorase on quinone-mediated genotoxicity in human and fish cell lines, Mutat. Res., 360, 43, 10.1016/S0165-1161(96)90236-8

Zaleski, 1991, Metabolism of benzo[a]pyrene and (−)-trans-benzo[a]pyrene-7,8-dihydrodiol by freshly isolated hepatocytes from mirror carp, Carcinogenesis, 12, 167, 10.1093/carcin/12.2.167

Kennedy, 1994, The effects of temperature on the uptake and metabolism of benzo[a]pyrene in isolated gill cells of the gulf toadfish, Opsanus beta, Fish Physiol. Biochem., 13, 93, 10.1007/BF00004335

Cravedi, 1995, Growth hormone effect on xenobiotic-metabolizing activities of rainbow trout, Mar. Environ. Res., 39, 89, 10.1016/0141-1136(94)00019-L

Huuskonen, 1995, Time-course studies of the biotransformation enzymes in control rainbow trout when adjusting to new habitats, Mar. Environ. Res., 39, 79, 10.1016/0141-1136(94)00043-O

Kennedy, 1991, In vitro metabolism of benzo(a)pyrene in the blood of the gulf toadfish, Opsanus beta, Mar. Environ. Res., 31, 37, 10.1016/0141-1136(91)90004-R

Fisher, 1995, Evaluation of the effects of cryopreservation of isolated erythrocytes and leukocytes of largemouth bass by flow cytometry, J. Fish Biol., 46, 432, 10.1111/j.1095-8649.1995.tb05983.x

Vukmirovic, 1994, DNA damage in marine mussel Mytilus galloprovincialis as a biomarker of environmental contamination, Mar. Ecol. Prog. Ser., 109, 165, 10.3354/meps109165

Everaarts, 1993, Biological markers in fish: DNA integrity, hematological parameters and liver somatic index, Mar. Environ. Res., 35, 101, 10.1016/0141-1136(93)90021-Q

Everaarts, 1994, Molecular responses as indicators of marine pollution—DNA damage and enzyme-induction in Limanda limanda and Asterias rubens, Environ. Health Perspect., 94, 37, 10.1289/ehp.94102s1237a

Everaarts, 1995, DNA integrity as a biomarker of marine pollution: Strand breaks in seastar (Asterias rubens) and dab (Limanda limanda), Mar. Pollut. Bull., 31, 431, 10.1016/0025-326X(95)00145-D

Sugg, 1996, DNA-damage and radiocesium in channel catfish from Chernobyl, Environ. Toxicol. Chem., 15, 1057, 10.1002/etc.5620150706