Confirmation by gas chromatography/mass spectrometry of two unusualrans‐3‐monoethylenic fatty acids from the nova scotian seaweedsPalmaria palmata andChondrus crispus

Lipids - Tập 29 Số 6 - Trang 441-444 - 1994
M. Lamberto1,2, R. G. Ackman1
1Canadian Institute of Fisheries Technology, Technical University of Nova Scotia, Box 1000, Halifax, Nova Scotia, B3J 2X4 Canada
2Department of Organic and Biological Chemistry, University of Messina, Messina, P.O. Box 46 Italy

Tóm tắt

Abstract

The structures of two unusual fatty acids, the knownrans‐3‐hexadecenoic acid and a noveltrans‐3‐tetradecenoic acid, both isolated from the Nova Scotian sea‐weedsPalmaria palmata andChondrus crispus, were positively identified. After the extraction of the total fatty acids by saponification, followed by methylation, the monoenoictrans fractions were isolated by thin‐layer chromatography on silica gel impregnated with silver nitrate. The monoenoictrans fractions were derivatized with 2‐amino‐2‐methyl‐propanol prior to analysis by gas chromatography/mass spectrometry. The mass spectra showed prominent [M+1]+ ions but lacked the 12 amu interval useful for identifying the double bond position. Hence, alternative diagnostic peaks were used to confirm the position of the double bond in these two fatty acids. As thetrans‐3‐hexadecenoic acid is found in the photosynthetic tissue of all plants, it may also be present in ruminant fats and, presumably, in human adipose tissue.

Từ khóa


Tài liệu tham khảo

10.1007/978-1-4899-2905-1

Weenink R.O., 1964, Biochim. Biophys. Acta, 84, 613

Araki S., 1986, Jap. J. Phycol., 34, 94

10.1016/S0031-9422(00)90096-7

Ackman R.G., 1979, Proceedings Ninth International Seaweed Symposium, 429

Ackman R.G., 1977, Proc. Nova Scotia Inst. Sci., 28, 42

Ratnayake W.M.N., 1984, Studies on Fatty Acids from Nova Scotia Seaweeds and on the Specificity of Hydrazine Reduction of Unsaturated Fatty Acids, 77

Ackman R.G., 1981, New Sources of Fats and Oils, 189

10.1007/BF02545311

10.1016/S0076-6879(81)72014-7

10.1016/S0022-2275(20)40190-7

10.1016/S0315-5463(81)72719-6

10.1007/BF02535015

10.1016/S0021-9673(01)95986-2

10.1007/BF02535640

10.1007/BF02536089

10.1002/bms.1200150106

10.1007/BF02532690

Christie W.W., 1989, Gas Chromatography and Lipids

Harvey D.J., 1992, Advances in Lipid Methodology—One, 19

Gurr M.I., 1984, Role of Fats in Food and Nutrition, 31

10.1007/BF02540496

Ackman R.G., 1986, Analysis of Oils and Fats, 137

10.1016/0308-8146(94)90091-4

10.1080/09528954.1987.11904111

10.1080/09528954.1987.11904112

Abbott I.A., 1988, Algae and Human Affairs, 138

Indergaard M., 1991, Seaweed Resources in Europe: Uses and Potential, 21

10.1007/BF02669970

10.1016/0378-4347(92)80269-V

10.1093/ajcn/58.2.228

Ackman R.G., 1989, Prog. Food Nutr. Sci., 13, 161

10.1002/lipi.19770790103

Norum K.R., 1989, The Role of Fats in Human Nutrition, 117, 10.1016/B978-0-12-718051-9.50008-1

Emken E.A., 1980, Handbook of Soy Oil Processing and Utilization, 439

Thông tin
Thông tin xuất bản

Lipids

Tập 29 Số 6

441-444

Thông tin tác giả