Comparative study of estradiol trapped in polymer gels versus estradiol conjugated to bovine serum albumin for production of polyclonal antibody

The aim of this work is the development of the immunogenic properties of 17beta-estradiol to produce polyclonal antibody for radioimmunoassay purposes. Poly(acrylamide–maleic acid) gel [P(AAm–MA)] was prepared by the template polymerization. Polyacrylamide (PAAm) was used as a template for the polymerization of MA in aqueous solution using gamma rays as initiator. Then a comparative study of estradiol trapped in the prepared polymer gels versus estradiol conjugated to bovine serum albumin (BSA) was carried out. Ten rats divided into two groups were injected according to specific protocol. The immunogenic properties of both produced polyclonal antibodies were studied. In conclusion, the results revealed that the similar immunogenic properties with specific radiolabeled tracer were obtained.