Cloning, sequencing, and expression of cscA invertase from Escherichia coli B-62

Canadian Journal of Microbiology - Tập 45 Số 5 - Trang 418-422 - 1999
Miklós Sahin‐Tóth1, Zsolt Lengyel, Hiroshi Tsunekawa
1Department of Physiology, University of California Los Angeles 90095-1662, USA.

Tóm tắt

We have isolated a 2.5-kb DNA fragment from plasmid pST5R7 encoding a sucrose utilization system from Escherichia coli B-62 which confers a sucrose-fermenting phenotype to transformed E. coli K-12 strains. DNA-sequence determination revealed one full-length open reading frame 98% identical to cscA, the sucrose-hydrolase (invertase) gene of the csc regulon from E. coli EC3132. Functional characterization indicates that high-level expression and limited periplasmic release of invertase is responsible for the sucrose-fermenting capacity of transformed E. coli K-12 strains carrying cscA.Key words: sucrose utilization, sucrose hydrolase, invertase, recombinant protein production.

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Thông tin
Thông tin xuất bản

Canadian Journal of Microbiology

Tập 45 Số 5

418-422

Thông tin tác giả