Cloning, sequence, and photoregulation of al-1, a carotenoid biosynthetic gene of Neurospora crassa.

Molecular and Cellular Biology - Tập 10 Số 10 - Trang 5064-5070 - 1990
Thomas J. Schmidhauser1,2, Frank‐Roman Lauter1,2, Vincenzo Russo1,2, Charles Yanofsky3
1Department of Biological Sciences, Stanford University, Stanford, California, 94305-5020
2Department of Chemistry and Biochemistry, Southern Illinois University, Carbondale, IL 62901-4409.
3and Max-Planck-Institut fur Molekulare Genetik, Abteilung Trautner, Ihnestrasse 73, D-1000 Berlin 33, Federal Republic of Germany2

Tóm tắt

Carotenoid biosynthesis is regulated by blue light during growth of Neurospora crassa mycelia. We have cloned the al-1 gene of N. crassa encoding the carotenoid-biosynthetic enzyme phytoene dehydrogenase and present an analysis of its structure and regulation. The gene encodes a 595-residue polypeptide that shows homology to two procaryotic carotenoid dehydrogenases. RNA measurements showed that the level of al-1 mRNA increased over 70-fold in photoinduced mycelia. Transcription run-on studies indicated that the al-1 gene was regulated at the level of initiation of transcription in response to photoinduction. The photoinduced increase of al-1 mRNA levels was not observed in two Neurospora mutants defective in all physiological photoresponses. Analysis of cosmid containing al-1 and of a translocation strain with a breakpoint within al-1 indicated that al-1 transcription proceeds towards the centromere of linkage group I of N. crassa.

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Molecular and Cellular Biology

Tập 10 Số 10

5064-5070

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