Nhân bản DNA bổ sung thụ thể mineralocorticoid ở người: Mối quan hệ cấu trúc và chức năng với thụ thể glucocorticoid

American Association for the Advancement of Science (AAAS) - Tập 237 Số 4812 - Trang 268-275 - 1987
Jeffrey L. Arriza1,2, Cary Weinberger1, Gail Cerelli1, Tom Glaser3, Barbara Handelin3, David E. Housman3, Ronald M. Evans1
1Gene Expression Laboratory, The Salk Institute for Biological Studies, San Diego CA 92138.
2University of California at San Diego, Department of Biology, La Jolla, CA 92093
3Massachusetts Institute of Technology, Center for Cancer Research, Cambridge, MA 02139.

Tóm tắt

Kỹ thuật lai giảm mức độ nghiêm ngặt với DNA bổ sung của thụ thể glucocorticoid ở người (hGR) đã được sử dụng để phân lập một gen mới mã hóa polypeptide dự đoán có trọng lượng phân tử 107 kilodalton. Những nghiên cứu về biểu hiện cho thấy khả năng của nó trong việc liên kết với aldosterone với ái lực cao và kích hoạt sự phiên mã gen phản ứng với aldosterone, từ đó xác nhận danh tính của nó là thụ thể mineralocorticoid ở người (hMR). Phân tử này cũng biểu hiện mức độ cao trong ái lực với glucocorticoid và kích thích promoter đáp ứng glucocorticoid. Hai thụ thể hMR và hGR gộp lại cung cấp sự đa dạng chức năng đáng ngạc nhiên, trong đó các đặc tính liên kết hormone, sự tương tác gen mục tiêu, và các mô hình biểu hiện đặc thù theo mô có thể được sử dụng theo một cách kết hợp để đạt được sự kiểm soát sinh lý học phức tạp.

Từ khóa

#thụ thể mineralocorticoid #thụ thể glucocorticoid #liên kết ái lực cao #kiểm soát sinh lý học #gene phiên mã #promoter đáp ứng glucocorticoid

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