Chlorhexidine Diacetate and Povidone‐iodine Cytotoxicity to Canine Embryonic Fibroblasts and Staphylococcus aureus

Veterinary Surgery - Tập 17 Số 4 - Trang 182-185 - 1988
ISIS R. SANCHEZ1, Kenneth E. Nusbaum2, Steven F. Swaim1,3, Anne S. Hale2, Ronald D. Henderson1, John A. McGuire4
1Department of Small Animal Surgery and Medicine
2Department of Pathobiology
3Scott-Ritchey Research Laboratory
4Department of Research Data Analysis (McGuire), Auburn University, Alabama

Tóm tắt

Chlorhexidine diacetate and povidone‐iodine were evaluated for fibroblast toxicity on a primary line of canine embryonic fibroblasts, and for bactericidal efficacy against Staphylococcus aureus. The cultured fibroblasts or S. aureus were exposed for 30 minutes to incremental dilutions of 0.5 to 0.0005% chlorhexidine diacetate, 5.0 to 0.05% povidone‐iodine, or physiologic buffered saline as a control. To determine survival, fibroblasts were trypsinized and counted; S. aureus colonies were counted on brain‐heart infusion agar. Survival for both groups was expressed by calculating the number of living cells in test dilutions as a percentage of the number in control cultures. Fibroblast survival occurred at chlorhexidine concentrations less than 0.013% and at povidone‐iodine concentrations less than 0.5% (p < 0.05). Significant S. aureus survival (p < 0.05) was noted at chlorhexidine concentrations less than 0.05% and povidone‐iodine concentrations less than 1.0%. These data showed that all bactericidal concentrations of chlorhexidine diacetate and povidone‐iodine were lethal to canine embryonic fibroblasts in vitro, whereas non‐lethal concentrations allowed significant bacterial survival.

Từ khóa


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Veterinary Surgery

Tập 17 Số 4

182-185

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