Chemiluminescence of bovine polymorphonuclear leucocytes during the periparturient period and relation with metabolic markers and bovine pregnancy-associated glycoprotein

Journal of Dairy Research - Tập 67 Số 2 - Trang 249-259 - 2000
Dagmar Hoeben1,2, E. Monfardini1,3, G. Opsomer4, Christian Burvenich1, Hilde Dosogne1, Aart de Kruif4, JF Beckers5
1Department of Physiology, Biochemistry and Biometrics, Milk Secretion and Mastitis Research Center, Faculty of Veterinary Medicine, University of Gent, Salisburylaan 133, B-9820 Merelbeke, Belgium
2Present address: Janssen Animal Health BVBA, Turnhoutseweg 30, B-2340 Beerse, Belgium.
3Present address: Institute of Animal Nutrition, Faculty of Veterinary Medicine, University of Milan, ViaTrentacoste 2, I-20134 Milano, Italy.
4Department of Obstetrics and Herd Health, Faculty of Veterinary Medicine, University of Gent, Salisburylaan 133, B-9820 Merelbeke, Belgium
5Department of Physiology of Reproduction, Faculty of Veterinary Medicine, University of Liège, Boulevard de Colonster 20, B-4000 Sart-Tilman, Belgium

Tóm tắt

The respiratory burst activity of polymorphonuclear leucocytes (PMN) was evaluated in eight Holstein cows from 8 weeks before until 6 weeks after calving by chemiluminescence (CL). The CL response started to decrease 1 week before parturition, reaching a minimum during the first 2 weeks after calving. From week 3 of lactation, CL increased again and returned to original levels by week 6 of lactation. Plasma concentrations of 3-hydroxybutyric acid, total bilirubin and bovine pregnancy-associated glycoprotein started to increase before parturition to reach a maximum during the first or second week of lactation. The concentrations of glutamic–oxaloacetic transaminase, lactate dehydrogenase, non-esterified fatty acids and bilirubin increased after calving, reaching a maximum during the second week. A small decrease in plasma cholesterol during the week before and after calving was followed by an increase. The CL response of the PMN showed significant temporal relationships with the plasma concentrations of 3-hydroxybutyric acid, bovine pregnancy-associated glycoprotein, bilirubin, glutamic–oxaloacetic transaminase, non-esterified fatty acids; that with cholesterol was nearly significant. This means that the change in the CL response with time coincided with the changes in plasma concentrations of these substances with time and that these changes were significantly related with each other. The results of this study show that the decreased respiratory burst activity of bovine PMN around parturition may be related to the extent of the metabolic and hormonal changes. Although the causative relationships are not proven, these results support earlier results suggesting that 3-hydroxybutyric acid and bovine pregnancy-associated glycoprotein may directly affect neutrophil function, whereas non-esterified fatty acids, cholesterol, bilirubin, and liver enzymes may have potential as diagnostic markers of impaired neutrophil function and consequently increased disease risk around parturition.

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Journal of Dairy Research

Tập 67 Số 2

249-259

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