Characterization of ompA Genotypes by Sequence Analysis of DNA from All Detected Cases of Chlamydia trachomatis Infections during 1 Year of Contact Tracing in a Swedish County

Journal of Clinical Microbiology - Tập 42 Số 4 - Trang 1641-1647 - 2004
Maria Lysén1, Anders Österlund2, Carl‐Johan Rubin1,3, Tina Persson4, Ingrid Persson4, Björn Herrmann1,3
1Section of Clinical Bacteriology, Department of Medical Sciences, University of Uppsala, Uppsala
2Department of Clinical Microbiology, Central Hospital, Växjö
3Section of Sexually Transmitted Infections, Department of Clinical Bacteriology, Swedish Institute for Infectious Disease Control, Solna, Sweden
4Department of Communicable Disease Control and Prevention, Central Hospital, Karlstad

Tóm tắt

ABSTRACT In this study we aimed to characterize the ompA gene by sequencing DNA from all detected cases of Chlamydia trachomatis infection in a Swedish county during 2001, in order to improve the efficiency of contact tracing. Approximately 990 bp of the ompA gene was amplified, and sequence analysis was achieved in 678 (94%) of 725 C. trachomatis -positive cases in this unselected population. The most prevalent genotype was serotype E (39%), followed by F (21%), G (11%), D (9%), K (9%), J (7%), H (2%), B (1%), and Ia (1%). Serotype E was found in five genotype variants, with the reference sequence comprising 96% of all E cases. Serotype D was the most variable, and of seven sequence variants, three were identified as recombinants with serotype E. Altogether 29 genetic variants were detected, and mutations and recombination events are discussed. Clinical manifestations were not associated with genotypes. Sequence variation was linked to sexual networks identified by contact tracing and improved epidemiological knowledge but was of limited benefit.

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Journal of Clinical Microbiology

Tập 42 Số 4

1641-1647

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