Characterization of affinity-labeled fluorescyl ligand to specifically-purified rabbit IgG antibodies

Immunochemistry - Tập 15 Số 12 - Trang 875-882 - 1978
Robert M. Watt1, Edward W. Voss1
1Department of Microbiology, University of Illinois, Urbana, IL 6180I, U.S.A.

Tóm tắt

Từ khóa


Tài liệu tham khảo

Chen, 1969, Fluorescent protein--dye conjugates II. Gamma globulin conjugated with various dyes, Archs Biochem. Biophys., 133, 263, 10.1016/0003-9861(69)90454-8

Fleet, 1969, Affinity labeling of antibodies with aryl nitrene as reactive group, Nature, 224, 511, 10.1038/224511a0

Fleischman, 1962, Reduction of-γ-globulins, Archs Biochem. Biophys., 1, 174

Gill, 1967, Partition and ion-exchange thin-layer chromatography of water-soluble fluorescent compounds, J. Chromatog., 26, 315, 10.1016/S0021-9673(01)98881-8

Goetzel, 1970, Affinity labeling of a mouse myeloma protein which binds nitrophenyl ligands. Kinetics of labeling and isolation of a labeled peptide, Biochemistry, 9, 1267, 10.1021/bi00807a031

Goldman, 1968, 124

Gollogly, 1974, Sequential appearance of three different anti-fluorescein combining sites in hyperimmunized rabbits: characterization by circular dichroism and binding studies, J. Immun., 113, 1457, 10.4049/jimmunol.113.5.1457

Gruber, 1975, Dynamic and static quenching of 1,N6-ethenoadenine fluorescence in nicotinamide 1,N6-ethenoadenine dinucleotide and in 1,N6-etheno-9-[3-(indol-3-yl) propyl] adenine, 72, 3966

Haimovich, 1970, Affinity labeling of the heavy and light chains of a myeloma protein with anti-2,4-dinitrophenyl activity, 67, 1656

Karush, 1962, Immunological specificity and molecular structure, Vol. 2, 1

Laemmli, 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680, 10.1038/227680a0

Levison, 1971, Kinetic behavior of antihapten antibody of restricted heterogeneity by stopped flow fluorescence polarization kinetics, Biochem. biophys. Res. Commun., 43, 258, 10.1016/0006-291X(71)90746-7

Lopatin, 1971, Fluorescein: hapten and antibody active-site probe, Biochemistry, 10, 208, 10.1021/bi00778a003

Martin, 1973, Deuterium effects on radiationless electronic transitions of xanthane dyes in aqueous and alcoholic solvents, Chem. Phys. Lett., 22, 309, 10.1016/0009-2614(73)80100-9

Porter, 1973, Immunoglobulin Structure, 159

Portmann, 1971, Anti-fluorescein antibody of high affinity and restricted heterogeneity as characterized by fluorescence polarization and quenching equilibrium techniques, Biochem. biophys. Res. Commun., 43, 207, 10.1016/S0006-291X(71)80108-0

Press, 1971, Affinity labeling of rabbit antibodies with ɛ-4-azido-2-nitrophenyl lysine, Vol. 1, 233

Robbins, 1967, Purification of antibodies with immunoadsorbents prepared using bromoacetyl cellulose, Immunochemistry, 4, 11, 10.1016/0019-2791(67)90192-9

Scatchard, 1949, The attraction of proteins for small molecules and ions, Ann. N.Y. Acad. Sci., 51, 660, 10.1111/j.1749-6632.1949.tb27297.x

Singer, 1966, Antibody active sites and immunoglobulin molecules, Science, 153, 13, 10.1126/science.153.3731.13

Singer, 1971, Affinity labeling of the active sites of antibodies and myeloma proteins, Ann. N.Y. Acad. Sci., 190, 342, 10.1111/j.1749-6632.1971.tb13546.x

Sips, 1948, On the structure of a catalyst surface, J. Chem. Phys., 16, 490, 10.1063/1.1746922

Spencer, 1969, Measurements of subnanosecond fluorescence lifetimes with a crosscorrelation phase fluorometer, Ann. N.Y. Acad. Sci., 158, 361, 10.1111/j.1749-6632.1969.tb56231.x

Stevenson, 1970, The recombination of dimers of immunoglobulin peptide chains, Biochem. J., 118, 703, 10.1042/bj1180703

Stryer, 1966, Excited-state proton-transfer reactions. A deuterium isotope effect on fluorescence, J. Am. ehem. Soc., 88, 5708, 10.1021/ja00976a004

Studier, 1973, Analysis of bacteriophage T7 early RNAs and proteins on slab gels, J. molec. Biol., 79, 237, 10.1016/0022-2836(73)90003-X

Taussig, 1975, The antibody combining site, 42

Tengerdy, 1966, Optical absorption and fluorescence of fluorescent protein conjugates, Anal. Biochem., 16, 377, 10.1016/0003-2697(66)90218-1

Utsumi, 1964, The subunits of purified rabbit antibody, Biochemistry, 3, 1329, 10.1021/bi00897a024

Voss, 1976, Fluorescein: a complete antigenic group, Immuno-chemistry, 13, 237, 10.1016/0019-2791(76)90382-7

Voss, 1977, Comparison of the microenvironment of chicken and rabbit antibody active sites, 391

Voss, 1978

Watt, 1977, Mechanism of quenching of fluorescein by anti-fluorescein IgG antibodies, Immunochemistry, 14, 533, 10.1016/0019-2791(77)90308-1

Weber, 1957, Determination of the absolute quantum yield of fluorescent solutions, Trans. Faraday Soc., 53, 646, 10.1039/tf9575300646

Weber, 1964, Fragmentation of bovine serum albumin by pepsin. I. The origin of acid expansion of the albumin molecule, J. biol. Chem., 239, 1415, 10.1016/S0021-9258(18)91331-1

Wehrly, 1976, Bipolar averaging circuit for enhancing signal-to-noise ratios in recorded spectra, Analyt. Chem., 48, 1424, 10.1021/ac50003a041

Wofsy, 1962, Affinity labeling- a general method for labeling the active sites of antibody and enzyme molecules, Biochemistry, 1, 1031, 10.1021/bi00912a013