Characteristics and antifungal activity of a chitin binding protein from Ginkgo biloba

FEBS Letters - Tập 478 Số 1-2 - Trang 123-126 - 2000
Xu Huang1, Wei-jun Xie1, Zhao Gong1
1Shanghai Institute of Biochemistry, Chinese Academy of Sciences, 320 Yue-yang Road, Shanghai 200031, PR China

Tóm tắt

An antifungal peptide from leaves of Ginkgo biloba, designated GAFP, has been isolated. Its molecular mass of 4244.0 Da was determined by mass spectrometry. The complete amino acid sequence was obtained from automated Edman degradation. GAFP exhibited antifungal activity towards Pellicularia sasakii Ito, Alternaria alternata (Fries) Keissler, Fusarium graminearum Schw. and Fusarium moniliforme. Its activities differed among various fungi. GAFP could also cause increased hyphal membrane permeabilization and a rapid alkalization of the medium when applied at 100 μg/ml to Pellicularia sasakii Ito hyphae. The amino acid sequence of GAFP shows characteristics of the cysteine/glycine‐rich chitin binding domain of many chitin binding proteins. The cysteine residues are well conserved.

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FEBS Letters

Tập 478 Số 1-2

123-126

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