Case study development of a challenge test against Edwardsiella ictaluri in Mekong striped catfish (Pangasianodon hypophthalmus), for use in breeding: Estimates of the genetic correlation between susceptibilities in replicated tanks

Journal of Fish Diseases - Tập 44 Số 5 - Trang 553-561 - 2021
Khoi Dinh Pham1,2, Sang Van Nguyen2, Jørgen Ødegård3,1, Hans Magnus Gjøen1, G. Klemetsdal1
1Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, Ås, Norway
2Research Institute for Aquaculture No. 2 (RIA2), Ho Chi Minh City, Vietnam
3AquaGen AS, Trondheim, Norway

Tóm tắt

Abstract

Bacillary necrosis is a problematic disease in farming of Mekong striped catfish (Pangasianodon hypophthalmus). The pathogenic bacterium is Edwardsiella ictaluri, causing numerous white spots in swelled liver, kidney and spleen. An alternative to antibiotic treatment and vaccine is to select for improved genetic resistance to the disease that requires to establish a proper challenge test. Here, four challenge tests of Mekong striped catfish against E. ictaluri are reported proposing 3 days of acclimatization of test fish prior to the challenge, with restricted water level in the test, keeping a temperature of 26°C. In the challenge, cohabitant shedders should be released directly into the test tank and make up around ⅓ of the fish, and bacteria should be added directly to water. The last two experiments, with the highest mortality, suggest that any factor involving the dead cohabitants should be removed and that additional experimentation should focus on bacteria (density) and timing for addition of bacteria to water. Genetic analyses revealed that resistance to bacillary necrosis tested in replicated tanks in the same experiment can be considered the same genetic trait.

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Tài liệu tham khảo

Berg P., 1998, A comparison of mating designs for inference on genetic parameters in fish, Proc. 6th WCGALP, 27, 115

10.1006/fsim.2000.0261

10.1007/s40572-016-0091-2

10.1046/j.1365-2761.2002.00412.x

Dong H. T., 2008, Identification of the causative agent of “white patches in the internal organs disease” of Tra catfish in Ben Tre province

10.1016/j.aquaculture.2011.11.007

10.1016/j.vaccine.2013.01.016

10.1186/1297-9686-15-2-201

Gilmour A. R., 2015, ASReml user guide release 4.1 structural specification

10.1016/S0044-8486(97)00203-2

Hawke J. P., 1998, ESC‐ Enteric Septicemia of Catfish. Southern Regional Aquaculture Center, SRAC Publication No. 477

10.1016/j.aquatox.2011.03.019

10.1016/S0044-8486(97)00143-9

10.1016/j.aquaculture.2008.03.054

10.1016/S0044-8486(98)00426-8

10.1016/S0044-8486(97)00208-1

10.1111/j.1365-2109.2010.02669.x

10.1006/fsim.1999.0215

Pham K. D., 2020, Genetic analysis of resistance in Mekong striped catfish (Pangasianodon hypophthalmus) to bacillary necrosis caused by Edwardsiella ictaluri, Journal of Fish Diseases

10.1016/j.aquaculture.2012.03.013

Thanh P. C., 2012, Towards sustainability: Vaccinated pangasius in Vietnam, 36

10.1128/AEM.01115-07

10.1016/S0044-8486(98)00444-X

10.1016/j.aquaculture.2019.05.034

10.1111/jfd.13067

10.1016/j.aquaculture.2015.11.012

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Thông tin xuất bản

Journal of Fish Diseases

Tập 44 Số 5

553-561

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