Bioreactor technology for clonal propagation of plants and metabolite production

Frontiers in Biology - Tập 10 Số 2 - Trang 177-193 - 2015
Nazmul H. A. Mamun1, Ulrika Egertsdotter2, Cyrus K. Aidun2
1G. W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, USA
2G.W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA

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Tài liệu tham khảo

Akita M, Shigeoka T, Koizumi Y, Kawamura M (1994). Mass propagation of shoots of Stevia rebaudiana using a large scale bioreactor. Plant Cell Rep, 13: 180–183

Alvard D, Cote F, Teisson C (1993). Comparison of methods of liquid medium culture for banana micropropagation: effects of temporary immersion of explants. Plant Cell Tissue Organ Cult, 32(1): 55–60

Amanullah A, Serrano-Carreon L, Castro B, Galindo E, Nienow A W (1998). The influence of impeller type in pilot scale Xanthan fermentations. Biotechnol Bioeng, 57(1): 95–108

Arcuri E J, Nichols J R, Brix T S, Santamarina V G, Buckland B C, Drew S W (1983). Thienamycin production by immobilized cells of Streptomyces cattleya in a bubble column. Biotechnol Bioeng, 15(10): 2399–2411

Attree S M, Pomeroy M K, Fowke L C (1994). Production of vigorous, desiccation tolerant white spruce (Picea glauca [Moench.]Voss.) synthetic seeds in a bioreactor. Plant Cell Rep, 13(11): 601–606

Atwell B J, Greenway H (1987). The relationship between growth and oxygen uptake in hypoxic rice seedlings. J Exp Bot, 38(3): 454–465

Ballica R, Ryu D Y (1993). Effects of rheological properties and mass transfer on plant cell bioreactor performance: production of tropane alkaloids. Biotechnol Bioeng, 42(10): 1181–1189

Barry-Etienne D, Bertrand B, Schlonvoigt A, Etienne H (2002). The morphological variability within a population of coffee somatic embryos produced in a bioreactor affects the regeneration and the development of plants in the nursery. Plant Cell Tissue Organ Cult, 68(2): 153–162

Biddington N L, Robinson T H (1991). Ethylene production during anther culture of Brussel sprouts (Brassica oleracea var. gemmifera) and its relationship with factors that affect embryo production. Plant Cell Tissue Organ Cult, 25: 169–177

Bieniek ME, Harrell R C, Cantliffe D J (1995). Enhancement of somatic embryogenesis of Ipomoea batatas in solid cultures and production of mature somatic embryos in liquid cultures for application to a bioreactor production system. Plant Cell Tissue Organ Cult, 41(1): 1–8

Bramble J L, Graves D J, Brodelius P (1990). Calcium and phosphate effects on growth and alkaloid production in Coffea arabica experimental results and mathematical model. Biotechnol Bioeng, 37(9): 859–868

Burg S P (1973). Ethylene in plant growth. Proc Nat Acad Sci, USA, 70(2): 591–597

Cardillo A B, Otálvaro A Á M, Busto V D, Talou J R, Velásquez L M E, Giulietti A M (2010). Scopolamine, anisodamine and hyoscyamine production by Brugmansia candida hairy root cultures in bioreactors. Process Biochem, 45(9): 1577–1581

Chen H B, Kao P M, Huang H C, Shieh C J, Chen C I, Liu Y C (2010). Effects of using various bioreactors on chitinolytic enzymes productin by Paenibacillus taichungensis. Biochem Eng J, 49(3): 337–342

Chen S Y, Huang S Y (2000). Shear stress effects on cell growth and L-DOPA production by suspension culture of Stizolobium hassjoo cells in an agitated bioreactor. Bioprocess Eng, 22(1): 5–12

Cho J M, Kwon J Y, Lim J A, Kim D I (2007). Increased hGM-CSF production and secretion with pluronic F-68 in transgenic Nicotiana tabacum suspension cell cultures. Biotechnol Bioprocess Eng., 12(6): 594–600

Choi H J, Tao B Y, Okos M R (1995). Enhancement of secondary metabolite production by immobilized Gossypium arboreum cells. Biotechnol Prog, 11(3): 306–311

Choi Y S, Lee S Y, Kim D I (1999). Cultivation of Digitalis lanata cell suspension in an aqueous two-phase system. J Microbiol Biotechnol, 9(5): 589–592

Curtis W R (2005). Application of bioreactor design principles to plant micropropagation. Plant Cell Tissue Organ Cult, 81(3): 255–264

De Dobbeleer C, Cloutier M, Fouilland M, Legros R, Jolicoeur M (2006). A high-rate perfusion bioreactor for plant cells. Biotechnol Bioeng, 95(6): 1126–1137

Debergh P, Aitken-Christie J, Cohen D, Grout B, Arnold S, Zimmerman R, Ziv M (1992). Reconsideration of the term ‘vitrification’ as used in micropropagation. Plant Cell Tissue Organ Cult, 30(2): 135–140

Debergh P, Harbaoui Y, Lemeur R (1981). Mass propagation of globe artichoke (Cynara scolymus): evaluation of different hypotheses to overcome vitrification with special reference to water potential. Physiol Plant, 53(2): 181–187

Domínguez A, Rivela I, Couto S R, Sanromán M A (2001). Design of a new rotating drum bioreactor for ligninolytic enzyme production by Phanerochaete chrysosporium grown on an inert support. Process Biochem, 37(5): 549–554

Doran P M (1999). Design of mixing systems for plant cell suspensions in stirred reactors. Biotechnol Prog, 15(3): 319–335

Egertsdotter U, Mo L H, von Arnold S (1993). Extracellular proteins in embryogenic suspension cultures of Norway spruce (Picea abies). Physiol Plant, 88(2): 315–321

Egertsdotter U, von Arnold S (1995). Importance of arabinogalactan proteins for the development of somatic embryos of Norway spruce (Picea abies). Physiol Plant, 93(2): 334–345

Egertsdotter U, von Arnold S (1998). Development of somatic embryos in Norway spruce. J Exp Bot, 49(319): 155–162

Eibl R, Kaiser S, Lombriser R, Eibl D (2010). Disposable bioreactors: the current state-of-the-art and recommended applications in biotechnology. Appl Microbiol Biotechnol, 86(1): 41–49

El-Sayed A H M M, Rehm H J (1987). Continuous penicillin production by Penicilliumchrysogenum immobilized in calcium alginate beads. Appl Microbiol Biotechnol, 26(3): 215–218

Escalona M, Lorenzo J C, Gonzales B L, Daquinta M, Borroto C G, Gonzales J I, Desjardine Y (1999). Pineapple (Ananas cosmos L. Merr) micropropagation in temporary immersion systems. Plant Cell Rep, 18: 743–748

Fischer U, Santore U J, Husemann W, Barz W, Alfermann A W (1994). Semicontinuous cultivation of photoautotrophic cell-suspension cultures in a 20-L airlift-reactor. Plant Cell Tissue Organ Cult, 38(2–3): 123–134

Fu C C, Wu W T, Lu S Y (2003). Performance of airlift bioreactors with net draft tube. Enzyme Microb Technol, 33(4): 332–342

Fujimura M, Kato J, Tosa T, Chibata I (1984). Continuous production of L-arginine using immobilized growing Serratia marcescens cells: Effectiveness of supply of oxygen gas. Appl Microbiol Biotechnol, 19(2): 79–84

Fung C J, Metchell D A (1995). Baffles increase performance of solid-state fermentation in rotating drum bioreactors. Biotechnol Tech, 9(4): 295–298

Gagnon H, Thibault J, Cormier F, Do C B (1999). Vitis vinifera culture in a non-conventional bioreactor: the reciprocating plate bioreactor. Bioprocess Eng, 21(5): 405–413

Gaspar T (1991). Vitrification in micropropagation. In: Bajaj Y P S (Ed). Biotechnology in Agriculture and Forestry (vol.17). Berlin: Springer-Verlag, 117–126

Gaspar T, Kevers C, Debergh P, Maene L, Paques M, Boxus P (1987). Vitrification: morphological, physiological, and ecological aspects. In: Bonga J M, Durzan D J (Eds). Cell and Tissue Culture in Forestry (vol. 1). Dordrecht, Holland: Martinus Nijhoff Publishing, 152–166

Gaspar T, Kevers C, Franck T, Bisbis B, Billar J P, Huault C, Dily F L, Petit-Paly G, Rideau M, Penel C, Crevecoeur M, Greppin H (1995). Paradoxical results in the analysis of hyperhydric tissues considered as being under stress: questions for a debate. Bulg J Plant Physiol., 21(2–3): 80–97

Han J, Zhong J J (2003). Effects of oxygen partial pressure on cell growth and ginsenoside and polysaccharide production in high density cell cultures of Panax notoginseng. Enzyme Microb Technol, 32(3–4): 498–503

Hohe A, Winkelmann T, Schwenkel H G (1999). CO2 accumulation in bioreactor suspension cultures of Cyclamen persicum Mill. and its effect on cell growth and regeneration of somatic embryos. Plant Cell Rep, 18(10): 863–867

Honda H, Hiraoka K, Nagamori E, Omote M, Kato Y, Hiraoka S, Hobayashi T (2002). Enhanced anthocyanin production from grape callus in an air-lift type bioreactor using a viscous additive-supplemented medium. J Biosci Bioeng, 94(2): 135–139

Hooker B S, Lee J M, An G (1990). Cultivation of plant cells in a stirred vessel: effect of impeller design. Biotechnol Bioeng, 35(3): 296–304

Hu W W, Yao H, Zhong J J (2001). Improvement of Panax notoginseng cell culture for production of ginseng saponin and polysaccharide by high density cultivation in pneumatically agitated bioreactors. Biotechnol Prog, 17(5): 838–846

Hu W W, Zhong J J (2001). Effect of bottom clearance on performance of airlift bioreactor in high-density culture of Panax notoginseng cells. J Biosci Bioeng, 92(4): 389–392

Huang S Y, Shen Y W, Chan H S (2002). Development of a bioreactor operation strategy for L-DOPA production using Stizolobium hassjoo suspension culture. Enzyme Microb Technol, 30(6): 779–791

Huang T K, McDonald K A (2009). Bioreactor engineering for recombinant protein production in plant cell suspension cultures. Biochem Eng J, 45(3): 168–184

Hvoslef-Eide A K, Olsen O A S, Lyngved R, Munster C, Heyerdahl P H (2005). Bioreactor design for propagation of somatic embryos. Plant Cell Tissue Organ Cult, 81(3): 265–276

Illing S, Harrison S T L (1999). The kinetics and mechanism of Corynebacterium glutamicum aggregate breakup in bioreactors. Chem Eng Sci, 54(4): 441–454

Ingram B, Mavituna F (2000). Effect of bioreactor configuration on the growth and maturation of Picea sitchensis somatic embryo cultures. Plant Cell Tissue Organ Cult, 61(2): 87–96

Jackson MB, Fenning TM, Drew MC, Saker L R (1985). Stimulation of ethylene production and gas-space (aerenchyma) formation in adventitious roots of Zea mays L. by small partial pressures of oxygen. Planta, 165(4): 486–492

Jay V, Genestier S, Courduroux J C (1992). Bioreactor studies on the effect of dissolved oxygen concentrations on growth and differentiation of carrot (Daucus carota L.) cell cultures. Plant Cell Rep, 11(12): 605–608

Jay V, Genestier S, Courduroux J C (1994). Bioreactor studies on the effect of medium pH on carrot (Daucus carota L.) somatic embryogenesis. Plant Cell Tissue Organ Cult, 36(2): 205–209

Jianfeng X, Jian X, Aiming H, Pusun F, Zhiguo S (1998). Kinetic and technical studies on large-scale culture of Rhodiola sachalinensis compact callus aggregates with air-lift reactors. J Chem Technol Biotechnol, 72(3): 227–234

Jiménez E, Pérez N, de Feria M, Barbón R, Capote A, Chavez M, Quiala E, Pérez J C (1999). Improved production of potato microtubers using a temporary immersion system. Plant Cell Tissue Organ Cult, 59(1): 19–23

Jolicoeur M, Chavarie C, Carreau P J, Archambault J (1992). Development of a helical-ribbon impeller bioreactor for high-density plant cell suspension culture. Biotechnol Bioeng, 39(5): 511–521

Kantarci N, Borak F, Ulgen K O (2005). Bubble column reactors. Process Biochem, 40(7): 2263–2283

Kessell R H J, Carr A H (1972). The effect of dissolved oxygen concentration on growth and differentiation of carrot (Daucus carota) tissue. J Exp Bot, 23(4): 996–1007

Keßler M, ten Hoopen J G, Furusaki S (1999). The effect of the aggregate size on the production of ajmalicine and tryptamine in Catharanthus roseus suspension culture. Enzyme Microb Technol, 24(5–6): 308–315

Kevers C, Coumans M, Coumans-Gilles M F, Gasper T (1984). Physiological and biochemical events leading to vitrification of plants cultured in vitro. Physiol Plant, 61(1): 69–74

Kim J H, Yoo Y J (2002). Optimization of SOD biosynthesis by controlling sucrose concentration in the culture of carrot hairy root. J Microbiol Biotechnol, 12(4): 617–621

Kino-Oka R, Hitaka Y, Taya M, Tone S (1999). High-density culture of red beet hairy roots by considering medium flow condition in a bioreactor. Chem Eng Sci, 54(15–16): 3179–3186

Klvana M, Legros R, Jolicoeur M (2005). In situ extraction strategy affects benzophenanthridine alkaloid production fluxes in suspension cultures of Eschscholtzia californica. Biotechnol Bioeng, 89(3): 280–289

Kurata H, Furusaki S (1993). Immobilized Coffea arabica cell culture using a bubble-column reactor with controlled light intensity. Biotechnol Bioeng, 42(4): 494–502

Langer E S (2011). Trends in perfusion bioreactors-the next revolution in bioprocessing. BioProcess Int., 9(10): 18–22

Lee-Parsons C W, Shuler M L (2002). The effect of ajmalicine spiking and resin addition timing on the production of indole alkaloids from Catharanthus roseus cell cultures. Biotechnol Bioeng, 79(4): 408–415

Loc N H, Tuan C V, Binh D H N, Phuong T T B, Kim T G, Yang M S (2009). Accumulation of sesquiterpenes and polysaccharides in cells of zedoary (Curcuma zedoaria Roscoe) cultured in a 10 L bioreactor. Biotechnol Bioprocess Eng., 14(5): 619–624

Lorenzo J C, Gonzalez B L, Escalona M, Teisson C, Espinosa P, Borroto C (1998). Suggarcane shoot formation in an improved temporary immersion system. Plant Cell Tissue Organ Cult, 54(3): 197–200

Luo J, Mei X G, Liu L, Hu D W (2002). Improved paclitaxel production by fed-batch suspension cultures of Taxus chinensis in bioreactors. Biotechnol Lett, 24(7): 561–565

Luttman R, Florek P, Preil W (1994). Silicone-tubing aerated bioreactors for somatic embryo production. Plant Cell Tissue Organ Cult, 39(2): 157–170

Mandels M (1972). The culture of plant cells. Adv Biochem Eng, 2: 201–215

McAlister B, Finnie J, Watt M P, Blakeway F (2005). Use of the temporary immersion bioreactor system (RITA (R)) for production of commercial Eucalyptus clones in Mondi Forests (SA). Plant Cell Tissue Organ Cult, 81(3): 347–358

McDonald K A, Hong L M, Trombly D M, Xie Q, Jackman A P (2005). Production of human alpha-1-antitrypsin from transgenic rice cell culture in a membrane bioreactor. Biotechnol Prog, 21(3): 728–734

Molle F, Fressinet G (1992). Les semences artificielles. Phytoma., 441: 39–44

Mordocco AM, Brumbley J A, Lakshmanan P (2009). Development of a temporary immersion system (RITAA (R)) for mass production of sugarcane (Saccharum spp. interspecific hybrids). In Vitro Cell Dev Biol Plant, 45(4): 450–457

Mostafa S S, Gu X S (2003). Strategies for improved dCO2 removal in large-scale fed-batch cultures. Biotechnol Prog, 19(1): 45–51

Namdev P K, Dunlop E H (1995). Shear sensitivity of plant cells in suspnesions-present and future. Appl Biochem Biotechnol, 54(1–3): 109–131

Niemenak N, Saare-Surminski K, Rohsius C, Ndoumou D O, Lieberei R (2008). Regeneration of somatic embryos in Theobroma cacao L. in temporary immersion bioreactor and analyses of free amino acids in different tissues. Plant Cell Rep, 27(4): 667–676

Ogbonna J C, Mashima H, Tanaka H (2001). Scale up of fuel ethanol production form sugar beet juice using loofa sponge immobilized bioreactor. Bioresour Technol, 76(1): 1–8

Pal S, Das S, Dey S (2003). Peroxidase and arabinogalactan protein as by-products during somatic embryo cultivation in air-lift bioreactor. Process Biochem, 38(10): 1471–1477

Pan Z W, Wang H Q, Zhong J J (2000). Scale-up study on suspension cultures of Taxus chinensis cells for production of taxanediterpene. Enzyme Microb Technol, 27(9): 714–723

Pandey A (1992). Recent process developments in solid-state fermentation. Process Biochem, 27(2): 109–117

Paques M, Boxus P (1987). Vitrification: review of literature. Acta Hortic, 212: 155–166

Perata P, Alpi A (1991). Ethanol-induced injuries to carrot cells-the role of acetaldehyde. Plant Physiol, 95(3): 748–752

Pérez A, Nápoles L, Carvajal C, Hernandez M, Lorenzo J C (2004). Effect of sucrose, inorganic salts, inositol, and thiamine on protease excretion during pineapple culture in temporary immersion bioreactors. In Vitro Cell Dev Biol Plant, 40(3): 311–316

Piehl G W, Berlin J, Mollenschott C, Lehmann J (1988). Growth and alkaloid production of a cell suspension culture of Thalictrum rugosum in shake flasks and membrane-stirrer reactors with bubble free aeration. Appl Microbiol Biotechnol, 29(5): 456–461

Prakash G, Srivastava A K (2008). Statistical elicitor optimization studies for the enhancement of azadirachtin production in bioreactor Azadirachta indica cell cultivation. Biochem Eng J, 40(2): 218–226

Preil W (1991). Application of bioreactors in plant micropropagation. In: Debergh P C, Zimmerman R H (Eds). Micropropagation, technology and application. Dordrecht, Netherlands: Kluwer Academic Publishers, 425–455

Preil W, Florek P, Wix U, Beck A (1988). Towards mass propagation by use of bioreactors. Acta Hortic, 226: 99–106

Rosensweig R E (1979). Fluidization: Hydrodynamic stabilization with a magnetic field. Science, 204(6): 57–60

Rout G R, Mohapatra A, Jain S M (2006). Tissue culture of ornamental pot plant: A critical review on present scenario and future prospects. Biotechnol Adv, 24(6): 531–560

Rout G R, Samantaray S, Das P (2000). In vitro manipulation and propagation of medicinal plants. Biotechnol Adv, 18(2): 91–120

Sajc L, Vunjak-Novakovic G, Grubisic D, Kovačević N, Vuković D, Bugarski B (1995). Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor. Appl Microbiol Biotechnol, 43(3): 416–423

Schlatmann J E, Nuutila A M, van Gulik W M, ten Hoopen H J G, Verpoorte R, Heijnen J J (1993). Scaleup of ajmalicine production by plant cell cultures of Catharanthus roseus. Biotechnol Bioeng, 41(2): 253–262

Schubert S, Schubert E, Mengel K (1990). Effect of low pH of the root medium on proton release, growth, and nutrient uptake of field beans (Vicia faba). Plant Soil, 124(2): 239–244

Scragg A H (1995). The problems associated with high biomass levels in plant cell suspensions. Plant Cell Tissue Organ Cult, 43(2): 163–170

Seki M, Ohzora C, Takeda M, Furusaki S (1997). Taxol (paclitaxel) production using free and immobilized cells of Taxus cuspidate. Biotechnol Bioeng, 53(2): 214–219

Seydel P, Christian W, Heike D (2009). Scale-up of Oldenlandia affinis suspension cultures in photobioreactors for cyclotide production. Eng Life Sci, 9(3): 219–226

Shi Z D, Yuan Y J, Wu J C, Shang G M (2003). Biological responses of suspension cultures of Taxus chinensis var. mairei to shear stresses in the short term. Appl Biochem Biotechnol, 110(2): 61–74

Sim S J, Chang H N (1993). Increased shikonin production by hairy roots of Lithospermum erythrorhizon in 2 phase bubble column reactor. Biotechnol Lett, 15(2): 145–150

Singh V (1999). Disposable bioreactor for cell culture using wave-induced agitation. Cytotechnology, 30(1/3): 149–158

Small J G, Potgiether G P, Botha F C (1989). Anoxic seed germination of Erythrina caffra: Ethanol fermentation and response to metabolic inhibitors. J Exp Bot, 40(3): 375–381

Smart N J, Fowler M W (1984). Mass cultivation of Catharanthus roseus cells using a nonmechanically agitated bioreactor. Appl Biochem Biotechnol, 9(3): 209–216

Su W W, Arias R (2003). Continuous plant cell perfusion culture: bioreactor characterization and secreted enzyme production. J Biosci Bioeng, 95(1): 13–20

Su W W, He B J, Liang H, Sun S (1996). A perfusion air-lift bioreactor for high density plant cell cultivation and secreted protein production. J Biotechnol, 50(2–3): 225–233

Su W W, Lei F, Kao N P (1995). High density cultivation of Anchusaofficinalis in a stirred-tank bioreactor with in situ filtration. Appl Microbiol Biotechnol, 44(3–4): 293–299

Sun H (2010). The effect of hydrodynamic stress on plant embryo development. Ph.D. thesis, Georgia Institute of Technology, Atlanta, GA

Sun X, Linden J C (1999). Shear stress effects on plant cell suspension cultures in a rotating wall vessel bioreactor. J Ind Microbiol Biotechnol, 22(1): 44–47

Tanaka H, Nishijima F, Suwa M, Iwamoto T (1983). Rotating drum fermentor for plant cell suspension cultures. Biotechnol Bioeng, 25(10): 2359–2370

Teng WL, Liu Y J, Tsai Y C, Soong T S (1994). Somatic embryogenesis of carrot in bioreactor culture systems. HortScience, 29(11): 1349–1352

Terashima M, Ejirim Y, Hashikawa N, Yoshida H (2000). Effects of sugar concentration on recombinant human alpha(1)-antitrypsin production by genetically engineered rice cell. Biochem Eng J, 6(3): 201–205

Terrier B, Courtois D, Henault N, Cuvier A, Bastin M, Aknin A, Dubreuil J, Petiard V (2007). Two new disposable bioreactors for plant cell culture: the wave and undertow bioreactor and the slug bubble bioreactor. Biotechnol Bioeng, 96(5): 914–923

Thanh N T, Murthy H N, Pandey D M, Yu K W, Hahn E J, Paek K Y (2006b). Effect of carbon dioxide on cell gwowth and saponin production in suspension cultures of Panax ginseng. Biol Plant, 50(4): 752–754

Thomas D S, Murashige T (1979). Volatile emissions of plant tissue cultures. I. Identification of the major components. In Vitro, 15(9): 654–658

Tikhomiroff C, Allais S, Klvana M, Hisiger S, Jolicoeur M (2002). Continuous selective extraction of secondary metabolites from Catharanthus roseus hairy roots with silicon oil in a two-liquid-phase bioreactor. Biotechnol Prog, 18(5): 1003–1009

Tisserat B, Murashige T (1977). Effects of ethephon, ethylene, and 2,4-Dichlorophenoxyacetic acid on asexual embryogenesis in vitro. Plant Physiol, 60(3): 437–439

Tisserat B, Vandercook C E (1985). Development of an automated plant culture system. Plant Cell Tissue Organ Cult, 5(2): 107–117

Tokashiki M, Arai T, Hamamoto K, Ishimaru K (1990). High density culture of hybridoma cells using a perfusion culture vessel with an external centrifuge. Cytotechnology, 3(3): 239–244

Townsley P M, Webster F, Kutney J P, Salisbury P, Hewitt G, Kawamura N, Choi L, Kurihara T, Jacoli G G (1983). The recycling air lift transfer fermenter for plant cell. Biotechnol Lett, 5(1): 13–18

Treat W J, Engler C R, Soltes E J (1989). Culture of photomixotrophic soybean and pine in a modified fermenter using a novel impeller. Biotechnol Bioeng, 34(9): 1191–1202

Vinocur B, Carmi T, Altman A, Ziv M (2000). Enhanced bud regeneration in aspen (Populus tremula L.) roots cultured in liquid media. Plant Cell Rep, 19(12): 1146–1154

von Arnold S, Bozhkov P, Clapham D, Dyachok J, Filonova L, Högberg K A, Ingouff M, Wiweger M (2005). Propagation of Norway spruce via somatic embryogenesis. Plant Cell Tissue Organ Cult, 81: 323–329

Wang G R, Qi N M, Wang Z M (2010). Application of stir-tank bioreactor for perfusion culture and continuous harvest of Glycyrrhiza inflate suspension cells. Afr J Biotechnol, 9(3): 347–351

Wang S J, Zhong J J (1996). A novel centrifugal impeller bioreactor I. Fluid circulation, mixing, and liquid velocity profiles. Biotechnol Bioeng, 51(5): 511–519

Wang Z Y, Zhong J J (2002). Combination of conditioned medium and elicitation enhances taxoid production in bioreactor cultures of Taxus chinensis cells. Biochem Eng J, 12(2): 93–97

Williams R D, Chauret N, Bédard C, Archambault J (1992). Effect of polymeric adsorbents on the production of sanguinarine by Papaver somniferum cell-cultures. Biotechnol Bioeng, 40: 971–977

Wongsamuth R, Doran P M (1997). The filtration properties of Atropa belladonna plant cell suspensions; effects of hydrodynamic shear and elevated carbon dioxide levels on culture and filtration parameters. J Chem Technol Biotechnol, 69(1): 15–26

Yang R Y K, Bayraktar O, Pu H T (2003). Plant-cell bioreactors with simultaneous electropermeabilization and electrophoresis. J Biotechnol, 100(1): 13–22

Zhong C, Yuan Y J (2009). Responses of Taxus cuspidatato hydrodynamics in bubble column bioreactors with different sparging nozzle sizes. Biochem Eng J, 45(2): 100–106

Zhong J J, Fujiyama K, Seki T, Yoshida T (1994). A quantitative analysis of shear effects on cell suspension and cell culture of Perilla frutescens in bioreactors. Biotechnol Bioeng, 44(5): 649–654

Ziv M (1991). Vitrification: Morphological and physiological disorders of in vitro plants. In: Debergh P G, Zimmerman R H, (Eds). Micorpropagation: Technology and Application. Dordrecht, The Netherlands: Kluwer Academic Publishers, 45–69

Ziv M (2005). Simple bioreactors for mass propagation of plants. Plant Cell Tissue Organ Cult, 81(3): 277–285

Zobayed S M A, Saxena P K (2003). In vitro-grown roots: a superior explant for prolific shoot regeneration of St. Johns wort (Hypericum perforatum L. cv New Stem) in a temporary immersion bioreactor. Plant Sci, 165(3): 463–470