Bak Foong protects dopaminergic neurons against MPTP‐induced neurotoxicity by its anti‐apoptotic activity

Cell Biology International - Tập 32 - Trang 86-92 - 2008
Bin Liu1,2, Jun Xia Xie2, Lai Ling Tsang1, Dewi Kenneth Rowlands1, Lok Sze Ho1, Yu Lin Gou1, Yiu Wa Chung1, Hsiao Chang Chan1
1Epithelial Cell Biology Research Centre, Department of Physiology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong
2Department of Physiology, Medical College, Qingdao University, Qingdao 266021, PR China

Tóm tắt

AbstractBak Foong pill (BFP) is a well‐known traditional Chinese medicine used for treatment of various gynaecological disorders. In addition, it exerts beneficial effects on other functional systems including the central nervous system. In the present study, we have investigated the possible neuroprotective action of BFP upon the nigrostriatal dopaminergic system by examining its effect on the expression patterns of tyrosine hydroxylase (TH) and dopamine transporter (DAT) in the 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahyrdropyridine (MPTP)‐induced Parkinson's disease (PD) mouse model. MPTP significantly decreased TH and DAT mRNA levels in the striatum and midbrain of both female and male C57BL/6 mice. However, with BFP pre‐treatment mice showed a reduced neurotoxicity, with TH and DAT mRNA levels either not affected by MPTP or affected to a lesser extent in the midbrain and striatum when compared to vehicle treated animals. Possible anti‐apoptotic activity of BFP was further studied in a dopamine‐secreting neuroendocrine cell line, PC12. In this assay, MPTP elevated the expression of a pro‐apoptotic gene, Bax, while this expression was reduced by BFP pre‐treatment. Flow cytometry results also revealed that the effect of MPTP‐induced apoptosis in PC12 cell lines was significantly reduced by BFP. The present results suggest that BFP is able to protect dopaminergic neurons from neurotoxin‐induced neuronal injury with anti‐apoptotic activity being one of the possible mechanisms.

Tài liệu tham khảo

10.1016/0891-5849(91)90009-R

10.1046/j.1471-4159.1994.63020640.x

10.1016/S0165-6147(99)01392-9

10.1016/S0301-0082(01)00003-X

10.1212/WNL.52.6.1214

10.1073/pnas.80.14.4546

10.1016/0091-3057(94)00401-4

10.1016/0304-3940(96)12645-8

10.1212/WNL.40.5.763

10.1016/0006-291X(91)92101-O

Dluzen D.E., 2000, Gender differences in neurotoxicity of the nigrostriatal dopaminergic system: implications for Parkinson's disease, J Gend Specif Med, 3, 36

10.1016/0892-0362(96)00086-4

10.1248/bpb.26.241

10.1016/S0076-6879(79)58140-3

10.1046/j.1471-4159.1994.63051987.x

10.1016/0304-3940(96)12323-5

10.1126/science.6610213

10.1016/0304-3940(85)90580-4

10.1016/0306-4522(91)90180-V

10.1007/BF02802025

10.1016/1055-8330(95)90015-2

10.1073/pnas.82.7.2173

10.1016/j.cellbi.2005.03.025

10.1016/S0024-3205(98)00109-X

10.1126/science.6823561

10.1248/bpb.26.1028

10.1248/bpb.27.1245

10.1212/WNL.50.4.1141

10.1016/S0165-6147(99)01379-6

10.1016/0304-3940(94)90271-2

10.1016/S0306-4522(02)00578-X

10.1016/0024-3205(85)90146-8

10.1016/0092-8674(93)90509-O

10.1523/JNEUROSCI.13-10-04246.1993

Przedborski S., 1998, Mechanisms of MPTP toxicity, Mov Disord, 13, 35

10.1006/cbir.2001.0746

10.1016/S0149-7634(99)00059-7

10.1016/S0892-0362(02)00222-2

10.1016/S0006-8993(96)01271-1

10.1016/S0304-3940(96)12946-3

10.1002/ana.410440721

Tompkins M.M., 1997, Apoptotic‐like changes in Lewy‐body‐associated disorders and normal aging in substantia nigral neurons, Am J Pathol, 150, 119

10.1073/pnas.051633998

10.1248/bpb.26.1095

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Cell Biology International

Tập 32

86-92

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