Autoantibodies to Human Thyroid Peroxidase in Immunoassay and Immunoaffinity Chromatography

Springer Science and Business Media LLC - Tập 38 - Trang 83-88 - 2002
I. I. Vashkevich1, E. P. Kiseleva1, O. V. Sviridov1, L. I. Survilo1, O. A. Strel'chenok1
1Institute of Bioorganic Chemistry, National Academy of Sciences, Minsk, Belarus

Tóm tắt

A biochemical system of radioimmunoassay of human thyroid peroxidase (TPO) with the use of specific autoantibodies was developed for the first time. This system includes lyophilized preparations of human autoantibodies to TPO, radioiodinated TPO, standards prepared from pure TPO, and the solid-phase protein A as a precipitating agent. An analytical system was used to study the TPO distribution in fractions of thyroid tissue homogenate. Differences in TPO concentrations in mitochondria, microsomes, and supernatant fluid depending on thyroid pathology and tissue storage conditions were found. The behavior of immobilized anti TPO autoantibodies in immunoaffinity chromatography of this microsomal antigen was studied.

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Tài liệu tham khảo

Nunez, J., The Thyroid Gland, de Visscher, M., Ed., New York: Raven, 1980, pp. 39–59.

Czarnocka, B., Ruf, J., Ferrand, M., and Carayon, P., Thyroperoxidase and Thyroid Autoimmunity, Carayon, P. and Ruf, J., Eds., London: John Libbey Eurotext, 1990, vol. 207, pp. 59–67.

DeGroot, L.J., Thyroperoxidase and Thyroid Autoimmunity, Caarayon, P. and Ruf, J., Eds., London: John Libbey Eurotext, 1990, vol. 207, pp. 177–182.

McLachlan, S.M., Dickenson, A.M., Malkolm, A., Farndon, J.R., Young, E., Proctor, S.J., and Rees Smith, B., Clin. Exp. Immunol., 1983, vol. 52, no. 1, p. 45.

Doble, N.D., Banga, J.P., Pope, R., Lalor, E., Kilduff, P., and McGregor, A.M., Immunology, 1988, vol. 64, no. 1, pp. 23–29.

Okamoto, Y., Hamada, N., Saito, H., Ohno, M., Noh, J., Ito, K., and Mori, H., J. Clin. Endocrinol. Metab., 1989, vol. 68, no. 4, pp. 730–734.

Beever, K., Bradbury, J., Phillips, D., McLachlan, S.M., Pegg, C., Goral, A., Overbeck, W., Feifel, G., and Rees Smith, B., Clin. Chem. (Washingron, D. C.), 1989, vol. 35, no. 9, pp. 1949–1954.

Ohtaki, S., Nakagava, H., Nakamura, M., and Yamazaki, I., J. Biol. Chem., 1985, vol. 260, no. 1, pp. 441–448.

Wilkin, T.J. and Diaz, J.L., Thyroperoxidase and Thyroid Autoimmunity, Caarayon, P. and Ruf, J., Eds., London: John Libbey Eurotext, 1990, vol. 207, pp. 169–172.

Ruf, J., Ferrand, M., and Carayon, P., Thyroperoxidase and Thyroid Autoimmunity, Caarayon, P. and Ruf, J., Eds., London: John Libbey Eurotext, 1990, vol. 207, pp. 183–193.

Fukuma, N., Petersen, V.B., McLachlan, S.M., Pegg, C.A., and Rees Smith, B., Autoimmunity, 1991, vol. 10, no. 4, pp. 291–295.

Vashkevich, I.I., Ermolenko, M.N., Kiseleva, E.P., Mikhailopulo, K.I., and Sviridov, O.V., Biotekhnologiya, 1999, no. 3, pp. 43–55.

Ericson, L.E., Johanson, V., Molne, J., Nillson, M., and Ofverholm, T., Thyroperoxidase and Thyroid Autoimmunity, Caarayon, P. and Ruf, J., Eds., London: John Libbey Eurotext, 1990, vol. 207, pp. 107–116.

Czarnocka, B., Ruf, J., Ferrand, M., Carayon, P., and Lissitzky, S., FEBS Lett., 1985, vol. 190, no. 1, pp. 147–152.

Ruf, J., Toubert, M.-E., Czarnocka, B., Durand-Gorde, J.M., Ferrand, M., and Carayon, P., Endocrinology, 1989, vol. 125, no. 3, pp. 1211–1218.

Taurog, A., Dirris, M.L., Yokoyama, N., and Saughter, C., Arch. Biochem. Biophys., 1990, vol. 278, no. 2, pp. 333–341.

Dayan, C.M., Londei, M., Corcoran, A.E., Rapoport, B., and Feldmann, M., Thyroperoxidase and Thyroid Autoimmunity, Caarayon, P. and Ruf, J., Eds., London: John Libbey Eurotext, 1990, vol. 207, p. 267.

Vernov, F., Kalb, J., and Bernlohr, R., Anal. Biochem., 1977, vol. 82, no. 2, pp. 362–371.

Thacker, E.L., Davis, J.M., Refsal, K.R., and Bull, R.W., Am. J. Vet. Res., 1995, vol. 56, no. 1, pp. 34–38.

Ohtaki, S., Kotani, T., and Nakamura, Y., J. Clin. Endocrinol. Metab., 1986, vol. 63, no. 3, pp. 570–576.

Giraud, A., Franc, L.D., Long, Y., and Ruf, J., J. Endocrinol., 1992, vol. 132, no. 2, pp. 317–323.

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Springer Science and Business Media LLC

Tập 38

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