Arsenic detoxification and evolution of trimethylarsine gas by a microbial arsenite S -adenosylmethionine methyltransferase

Proceedings of the National Academy of Sciences of the United States of America - Tập 103 Số 7 - Trang 2075-2080 - 2006
Jie Qin1, Barry P. Rosen1, Yang Zhang2, Gejiao Wang2, Sylvia Franke2, Christopher Rensing2
1*Department of Biochemistry and Molecular Biology, Wayne State University, Detroit, MI 48201; and
2Department of Soil, Water, and Environmental Science, University of Arizona, Tucson, AZ 85721

Tóm tắt

In this article, a mechanism of arsenite [As(III)]resistance through methylation and subsequent volatization is described. Heterologous expression of arsM from Rhodopseudomonas palustris was shown to confer As(III) resistance to an arsenic-sensitive strain of Escherichia coli . ArsM catalyzes the formation of a number of methylated intermediates from As(III), with trimethylarsine as the end product. The net result is loss of arsenic, from both the medium and the cells. Because ArsM homologues are widespread in nature, this microbial-mediated transformation is proposed to have an important impact on the global arsenic cycle.

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Proceedings of the National Academy of Sciences of the United States of America

Tập 103 Số 7

2075-2080

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