Application of Response Surface Methodology for Maximizing Dextransucrase Production from Leuconostoc mesenteroides NRRL B-640 in a Bioreactor

Applied Biochemistry and Biotechnology - Tập 151 - Trang 182-192 - 2008
Ravi Kiran Purama1, Arun Goyal1
1Department of Biotechnology, Indian Institute of Technology Guwahati, Assam, India

Tóm tắt

The production of dextransucrase from Leuconostoc mesenteroides NRRL B-640 was investigated using statistical approaches. Plackett–Burman design with six variables, viz. sucrose, yeast extract, K2HPO4, peptone, beef extract, and Tween 80, was used to screen the nutrients that significantly affected the dextransucrase production. 24-Central composite design with four selected variables (sucrose, K2HPO4, yeast extract, and beef extract) was used for response surface methodology (RSM) for optimizing the enzyme production. The culture was grown under flask culture with 100 ml optimized medium containing 30 g/l sucrose, 18.5 g/l yeast extract, 15.3 g/l K2HPO4, and 5 g/l beef extract at 25 °C and shaking at 200 rpm gave dextransucrase with specific activity of 0.68 U/mg. Whereas the same optimized medium in a 3.0-l bioreactor (1.4 l working volume) gave an experimentally determined value of specific activity of 0.70 U/mg, which was in perfect agreement with the predicted value of 0.65 U/mg by the statistical model.

Tài liệu tham khảo

Remaud-Simeon, M., Willemot, R. M., Sarcabal, P., Potocki de Montalk, G., & Monsan, P. F. (2000). Journal of Molecular Catalysis B Enzymatic, 10, 177–198.

Van Hijum, S. A. F. T., Kralj, S., Ozimek, L. K., Dijkhuizen, L., & Van Geel-Schutten, G. H. I. (2006). Microbiology and Molecular Biology Reviews, 70, 157–176.

Robyt, J. F. (1995). Advances in Carbohydrate Chemistry and Biochemistry, 51, 133–168.

Lacaze, G., Wick, M., & Cappelle, S. (2007). Food Microbiology, 24, 155–160.

Purama, R. K., & Goyal, A. (2005). Indian Journal of Microbiology, 2, 89–101.

Naessens, M., Cerdobbel, A., Soetaert, W., & Vandamme, E. J. (2005). Journal of Chemical Technology & Biotechnology, 80, 845–860.

Robyt, J. F. (1986). Dextran. In H. F. Mark, N. M. Bikales, C. G. Overberger, & G. Menges (Eds.) Encyclopedia of polymer science and technology (vol. 4, (pp. 752–767)). New York: Wiley.

Goulas, A. K., Fisher, D. A., Grimble, G. K., Grandison, A. S., & Rastall, R. A. (2004). Enzyme and Microbial Technology, 35, 327–338.

Seo, E.-S., Nam, S.-H., Kang, H.-K., Cho, J.-Y., Lee, H.-S., Ryu, H.-W., et al. (2007). Enzyme and Microbial Technology, 40, 1117–1123.

Chung, C.-H., & Day, D. F. (2002). Journal of Industrial Microbiology & Biotechnology, 29, 196–199.

Tsuchiya, H. M., Koepsell, H. J., Corman, J., Bryant, G., Bogard, M. O., Feger, V. H., et al. (1952). Journal of Bacteriology, 64, 521–526.

Barker, P. E., & Ajongwen, N. J. (1991). Biotechnology & Bioengineering, 37, 703–707.

Goyal, A., & Katiyar, S. S. (1997). Journal of Basic Microbiology, 37, 197–204.

Santos, M., Teixeira, J., & Rodrigues, A. (2000). Biochemical Engineering Journal, 4, 177–188.

Behravan, J., Bazzaz, B. S. F., & Salimi, Z. (2003). Biotechnology and Applied Biochemistry, 38, 267–269.

Bas, D., & Boyaci, I. H. (2007). Journal of Food Engineering, 78, 836–845.

Majumder, A., & Goyal, A. (2007). Bioresource Technology. DOI 10.1016/j.biortech.2007.07.027.

Gangadharan. D., Sivaramakrishnan, S., Nampoothiri, K. M., Sukumaran, R. K., & Pandey, A. (2007). Bioresource Technology. DOI 10.1016/j.biortech.2007.07.028.

Rao, Y. K., Tsay, K.-J., Wu, W.-S., & Tzeng, Y.-M. (2007). Process Biochemistry, 42, 535–541.

Robyt, J. F., & Walseth, T. F. (1979). Carbohydrate Research, 68, 95–111.

Purama, R. K., & Goyal, A. (2008). Bioresource Technology. DOI 10.1016/j.biortech.2007.07.044.

DeMan, J. C., Rogosa, M., & Sharpe, M. E. (1961). Journal of Applied Bacteriology, 23, 130–135.

Nelson, N. (1944). Journal of Biological Chemistry, 153, 375–380.

Somogyi, M. (1945). Journal of Biological Chemistry, 160, 61–68.

Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Journal of Biological Chemistry, 193, 265–275.

Plackett, R. L., & Burman, J. P. (1946). Biometrika, 33, 305–325.

Haider, M. A., & Pakshirajan, K. (2007). Applied Biochemistry and Biotechnology, 141, 377–390.

Rao, J. L. U. M., & Satyanarayana, T. (2007). Bioresource Technology, 98, 345–352.

Thông tin
Thông tin xuất bản

Applied Biochemistry and Biotechnology

Tập 151

182-192

Thông tin tác giả