Anti‐quorum sensing activity of <i>Psidium guajava</i> L. flavonoids against <i>Chromobacterium violaceum</i> and <i>Pseudomonas aeruginosa</i> PAO1

Microbiology and Immunology - Tập 58 Số 5 - Trang 286-293 - 2014
H.S. Vasavi1, A. B. Arun1, P. D. Rekha1
1Yenepoya Research Centre, Yenepoya University, University Road, Nityananda Nagar, Deralakatte, Mangalore 575018, Karnataka, India.

Tóm tắt

ABSTRACTPsidium guajava L., which has been used traditionally as a medicinal plant, was explored for anti‐quorum sensing (QS) activity. The anti‐QS activity of the flavonoid (FL) fraction of P. guajava leaves was determined using a biosensor bioassay with Chromobacterium violaceum CV026. Detailed investigation of the effects of the FL‐fraction on QS‐regulated violacein production in C. violaceum ATCC12472 and pyocyanin production, proteolytic, elastolytic activities, swarming motility and biofilm formation in Pseudomonas aeruginosa PAO1 was performed using standard methods. Possible mechanisms of QS‐inhibition were studied by assessing violacein production in response to N‐acyl homoserine lactone (AHL) synthesis in the presence of the FL‐fraction in C. violaceum ATCC31532 and by evaluating the induction of violacein in the mutant C. violaceum CV026 by AHL extracted from the culture supernatants of C. violaceum 31532. Active compounds in the FL‐fraction were identified by liquid chromatography–mass spectrometry (LC–MS). Inhibition of violacein production by the FL‐fraction in a C. violaceum CV026 biosensor bioassay indicated possible anti‐QS activity. The FL‐fraction showed concentration‐dependent decreases in violacein production in C. violaceum 12472 and inhibited pyocyanin production, proteolytic and elastolytic activities, swarming motility and biofilm formation in P. aeruginosa PAO1. Interestingly, the FL‐fraction did not inhibit AHL synthesis; AHL extracted from cultures of C. violaceum 31532 grown in the presence of the FL‐fraction induced violacein in the mutant C. violaceum CV026. LC–MS analysis revealed the presence of quercetin and quercetin‐3‐O‐arabinoside in the FL‐fraction. Both quercetin and quercetin‐3‐O‐arabinoside inhibited violacein production in C. violaceum 12472, at 50 and 100 μg/mL, respectively. Results of this study provide scope for further research to exploit these active molecules as anti‐QS agents.

Từ khóa


Tài liệu tham khảo

10.1016/S0277-9536(98)00181-6

10.1016/S0031-9422(02)00190-5

10.1016/j.jep.2008.01.025

Vargas A.D., 2006, Kinetics of accumulation and distribution of flavonoids in guava (Psidium guajava), Agrociencia, 40, 109

Cheruiyot K.R., 2009, In‐vitro antibacterial activity of selected medicinal plants from Longisa region of Bomet district, Kenya, Afr Health Sci, 9, S42

Obinna N.C., 2008, Evaluation of antibacterial activity of Psidium guajava and Gongronema latifolium, J Med Plants Res, 2, 189

10.1271/bbb.66.1727

Rattanachaikunsopon P., 2010, Contents and antibacterial activity of flavonoids extracted from leaves of Psidium guajava, J Med Plants Res, 4, 393

10.1099/mic.0.049338-0

10.1021/jf301365a

10.1146/annurev.micro.55.1.165

10.1016/S0092-8674(02)00749-3

10.1128/JB.188.4.1451-1461.2006

10.1128/JB.187.5.1799-1814.2005

10.1016/j.jep.2011.01.028

10.1111/j.1574-6968.2007.01016.x

10.1016/S1369-5274(03)00008-0

10.1099/00221287-143-12-3703

10.1111/j.1472-765X.2006.01928.x

10.1128/jb.172.2.884-900.1990

10.1128/iai.38.2.716-723.1982

Mihalik K., 2008, Quorum sensing modulators of Pseudomonas aeruginosa characterized in Camellia sinensis, Asian J Trad Med, 3, 12

10.1016/j.fitote.2007.03.009

10.1128/AEM.01059-09

10.1016/S0031-9422(00)00035-2

10.1038/nchembio.2007.24

10.4049/jimmunol.174.6.3643

10.1007/s00216-006-0774-x

10.1371/journal.pone.0027091

10.1007/978-3-540-75418-3_4

10.2174/092986709787909640

10.1111/j.1365-2958.2006.05421.x

10.1038/nbt1206-1504

10.1111/j.1365-2672.2006.02912.x

10.1099/00221287-146-12-3237

10.2174/187152109789760117

10.1021/np100872k

10.1172/JCI20074

Lozoya X., 2002, Intestinal anti‐spasmodic effect of a phytodrug of Psidium guajava folia in the treatment of acute diarrheic disease, J Ethnopharmacol, 25, 235

10.1016/S0378-8741(99)00022-7

Thông tin
Thông tin xuất bản

Microbiology and Immunology

Tập 58 Số 5

286-293

Thông tin tác giả