Analyses of the mechanism of intracellular transport and secretion of pituitary hormone, with an insight of the subcellular localization of pituitary hormone and its mRNA

Brain Tumor Pathology - Tập 23 - Trang 1-5 - 2006
Akira Matsuno1, Akiko Mizutani2, Susumu Takekoshi3, Johbu Itoh4, Hiroko Okinaga5, Yuko Nishina5, Koji Takano5, Tadashi Nagashima1, R. Yoshiyuki Osamura3, Akira Teramoto6
1Department of Neurosurgery, Teikyo University Ichihara Hospital, Ichihara City, Japan
2Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Japan
3Department of Pathology, Tokai University School of Medicine, Isehara, Japan
4Teaching and Research Support Center, Tokai University School of Medicine, Isehara, Japan
5Department of Nephrology and Endocrinology, University of Tokyo Hospital, Tokyo, Japan
6Department of Neurosurgery, Nippon Medical School, Tokyo, Japan

Tóm tắt

Combined in situ hybridization (ISH) and immunohistochemistry (IHC) under electron microscopy (EM-ISH&IHC) has sufficient ultrastructural resolution and provides two-dimensional images of subcellular localization of pituitary hormone and its mRNA in a pituitary cell. The advantages of semiconductor nanocrystals (Quantum dots, Qdots) and confocal laser scanning microscopy (CLSM) enable us to obtain three-dimensional images of subcellular localization of pituitary hormone and its mRNA. Both EM-ISH&IHC and ISH&IHC using Qdots and CLSM are useful for understanding the relation between protein and mRNA simultaneously in two or three dimensions. Another important issue is the intracellular transport and secretion of pituitary hormone. We have developed an experimental pituitary cell line, the GH3 cell, which has growth hormone (GH) linked to enhanced yellow fluorescein protein (EYFP). This stable GH3 cell secretes GH linked to EYFP upon stimulated by Ca2+ influx or Ca2+ release from storage. This GH3 cell is useful for real-time visualization of the intracellular transport and secretion of GH. These three methods enable us to visualize consecutively the process of transcription, translation, transport, and secretion of pituitary hormone.

Tài liệu tham khảo

A Matsuno H Utsunomiya Y Ohsugi et al. (1996) ArticleTitleSimultaneous ultrastructural identification of growth hormone and its messenger ribonucleic acid using combined immunohistochemistry and non-radioisotopic in situ hybridization: a technical note Histochem J 28 703–707 Occurrence Handle8950599 Occurrence Handle1:STN:280:ByiC3cjoslA%3D Occurrence Handle10.1007/BF02409007 A Matsuno Y Ohsugi H Utsunomiya et al. (1998) ArticleTitleAn improved ultrastructural double-staining method of rat growth hormone and its mRNA using LR White resin: a technical note Histochem J 30 105–109 Occurrence Handle10192551 Occurrence Handle1:CAS:528:DyaK1cXisVCns7g%3D Occurrence Handle10.1023/A:1003283016889 A Matsuno T Nagashima RY Osamura et al. (1998) ArticleTitleApplication of ultrastructural in situ hybridization combined with immunohistochemistry to pathophysiological studies of pituitary cell: technical review Acta Histochem Cytochem 31 259–265 A Matsuno T Nagashima S Takekoshi et al. (1998) ArticleTitleUltrastructural simultaneous identification of growth hormone and its messenger ribonucleic acid Endocr J 45 IssueIDSuppl S101–S104 Occurrence Handle9790240 Occurrence Handle1:CAS:528:DyaK1cXks1WksL8%3D A Matsuno J Itoh RY Osamura et al. (1999) ArticleTitleElectron microscopic and confocal laser scanning microscopic observation of subcellular organelles and pituitary hormone mRNA: application of ultrastructural in situ hybridization and immunohistochemistry to the pathophysiological studies of pituitary cells Endocr Pathol 10 199–211 Occurrence Handle12114700 Occurrence Handle1:CAS:528:DyaK1MXotVCitrc%3D A Matsuno T Nagashima Y Ohsugi et al. (2000) ArticleTitleElectron microscopic observation of intracellular expression of mRNA and its protein product: technical review on ultrastructural in situ hybridization and its combination with immunohistochemistry Histol Histopathol 15 261–268 Occurrence Handle10668215 Occurrence Handle1:CAS:528:DC%2BD3cXhs1yiurg%3D RY Osamura Y Itoh A Matsuno (2000) ArticleTitleApplication of plastic embedding to electron microscopic immunocytochemistry and in situ hybridization in observations of production and secretion of peptide hormones J Histochem Cytochem 48 885–892 Occurrence Handle10858265 Occurrence Handle1:CAS:528:DC%2BD3cXksFyjt7g%3D RY Osamura S Tahara R Kurotani et al. (2000) ArticleTitleContributions of immunohistochemistry and in situ hybridization to the functional analysis of pituitary adenomas J Histochem Cytochem 48 445–458 Occurrence Handle10727285 Occurrence Handle1:CAS:528:DC%2BD3cXisVyms70%3D DJ Arndt-Jovin M Robert-Nicoud SJ Kaufman et al. (1985) ArticleTitleFluorescence digital imaging microscopy in cell biology Science 230 247–256 Occurrence Handle4048934 Occurrence Handle1:STN:280:BimD3Mfot1I%3D DJ Arndt-Jovin M Robert-Nicoud TM Jovin (1990) ArticleTitleProbing DNA structure and function with a multi-wavelength fluorescence confocal laser microscope J Microsc 157 61–72 Occurrence Handle2105397 Occurrence Handle1:CAS:528:DyaK3cXhvFCksrg%3D JG Bauman JA Bayer H van Dekken (1990) ArticleTitleFluorescent in-situ hybridization to detect cellular RNA by flow cytometry and confocal microscopy J Microsc 157 73–81 Occurrence Handle1688948 Occurrence Handle1:CAS:528:DyaK3cXhvFCltr8%3D P Hozak JT Novak K Smetana (1989) ArticleTitleThree-dimensional reconstructions of nucleolus-organizing regions in PHA-stimulated human lymphocytes Biol Cell 66 225–233 Occurrence Handle2605368 Occurrence Handle1:STN:280:By%2BC3crptFc%3D Occurrence Handle10.1016/0248-4900(89)90175-5 J Itoh RY Osamura K Watanabe (1992) ArticleTitleSubcellular visualization of light microscopic specimens by laser scanning microscopy and computer analysis: a new application of image analysis J Histochem Cytochem 40 955–967 Occurrence Handle1607644 Occurrence Handle1:CAS:528:DyaK38Xks1Grs7g%3D J Itoh N Sanno A Matsuno et al. (1997) ArticleTitleApplication of confocal laser scanning microscopy (CLSM) to visualize prolactin (PRL) and PRL mRNA in the normal and estrogen-treated rat pituitary glands using non-fluorescent probes Microsc Res Tech 39 157–167 Occurrence Handle9361267 Occurrence Handle1:CAS:528:DyaK2sXntlGjsrc%3D Occurrence Handle10.1002/(SICI)1097-0029(19971015)39:2<157::AID-JEMT7>3.0.CO;2-I J Itoh A Matsuno Y Yamamoto et al. (2001) ArticleTitleConfocal laser scanning microscopic imaging of subcellular organelles, mRNA, protein products, and the microvessel environment Acta Histochem Cytochem 34 285–297 Occurrence Handle1:CAS:528:DC%2BD3MXotlCrsb8%3D Occurrence Handle10.1267/ahc.34.285 E Michel JA Parsons (1990) ArticleTitleHistochemical and immunocytochemical localization of prolactin receptors on Nb2 lymphoma cells: applications of confocal microscopy J Histochem Cytochem 38 965–973 Occurrence Handle2355177 Occurrence Handle1:CAS:528:DyaK3cXltFWqsLw%3D JM Robinson BE Batten (1989) ArticleTitleDetection of diaminobenzidine reactions using scanning laser confocal reflectance microscopy J Histochem Cytochem 37 1761–1765 Occurrence Handle2555410 Occurrence Handle1:CAS:528:DyaK3cXjt1yjuw%3D%3D T Takamatsu S Fujita (1988) ArticleTitleMicroscopic tomography by laser scanning microscopy and its three-dimensional reconstruction J Microsc 149 167–174 Occurrence Handle3398041 W Tao RJ Walter MW Berns (1988) ArticleTitleLaser-transected microtubules exhibit individuality of regrowth, however most free new ends of the microtubules are stable J Cell Biol 107 1025–1035 Occurrence Handle3047148 Occurrence Handle1:STN:280:BieA3s7gvVw%3D Occurrence Handle10.1083/jcb.107.3.1025 JG White WB Amos M Fordham (1987) ArticleTitleAn evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy J Cell Biol 105 41–48 Occurrence Handle3112165 Occurrence Handle1:STN:280:BiiB1c%2Fjslw%3D Occurrence Handle10.1083/jcb.105.1.41 M Bruchez SuffixJr M Moronne P Gin et al. (1998) ArticleTitleSemiconductor nanocrystals as fluorescent biological labels Science 281 2013–2016 Occurrence Handle9748157 Occurrence Handle1:CAS:528:DyaK1cXmtlKgurw%3D Occurrence Handle10.1126/science.281.5385.2013 C Wang M Shim P Guyot-Sionnest (2001) ArticleTitleElectrochromic nanocrystal quantum dots Science 291 2390–2392 Occurrence Handle11264530 Occurrence Handle1:CAS:528:DC%2BD3MXit1KntLc%3D Occurrence Handle10.1126/science.291.5512.2390 WC Chan DJ Maxwell X Gao et al. (2002) ArticleTitleLuminescent quantum dots for multiplexed biological detection and imaging Curr Opin Biotechnol 13 40–46 Occurrence Handle11849956 Occurrence Handle1:CAS:528:DC%2BD38XhtF2itr0%3D Occurrence Handle10.1016/S0958-1669(02)00282-3 X Gao WC Chan S Nie (2002) ArticleTitleQuantum-dot nanocrystals for ultrasensitive biological labeling and multicolor optical encoding J Biomed Opt 7 532–537 Occurrence Handle12421118 Occurrence Handle1:CAS:528:DC%2BD38XotFyqsLk%3D Occurrence Handle10.1117/1.1506706 X Gao S Nie (2003) ArticleTitleMolecular profiling of single cells and tissue specimens with quantum dots Trends Biotechnol 21 371–373 Occurrence Handle12948664 Occurrence Handle1:CAS:528:DC%2BD3sXms1yhtrY%3D Occurrence Handle10.1016/S0167-7799(03)00209-9 M Han X Gao JZ Su et al. (2001) ArticleTitleQuantum-dot-tagged microbeads for multiplexed optical coding of biomolecules Nat Biotechnol 19 631–635 Occurrence Handle11433273 Occurrence Handle1:CAS:528:DC%2BD3MXkvFGjtrg%3D Occurrence Handle10.1038/90228 S Pathak SK Choi N Arnheim et al. (2001) ArticleTitleHydroxylated quantum dots as luminescent probes for in situ hybridization J Am Chem Soc 123 4103–4104 Occurrence Handle11457171 Occurrence Handle1:CAS:528:DC%2BD3MXisVOrsbs%3D Occurrence Handle10.1021/ja0058334 Y Xiao PE Barker (2004) ArticleTitleSemiconductor nanocrystal probes for human metaphase chromosomes Nucleic Acids Res 32 e28 Occurrence Handle14960711 Occurrence Handle10.1093/nar/gnh024 A Matsuno J Itoh S Takekoshi et al. (2005) ArticleTitleThree-dimensional imagings of the intracellular localization of growth hormone and prolactin and their mRNA using nanocrystal (Quantum dot) and confocal laser scanning microscopy techniques J Histochem Cytochem 53 833–838 Occurrence Handle15995141 Occurrence Handle1:CAS:528:DC%2BD2MXlvVWisbw%3D Occurrence Handle10.1369/jhc.4A6577.2005 A Matsuno J Itoh S Takekoshi et al. (2005) ArticleTitleTwo- or three-dimensional imagings of simultaneous visualization of rat pituitary hormone and its mRNA: comparison between electron microscopy and confocal laser scanning microscopy with nanocrystal (Quantum dot) Acta Histochem Cytochem 53 833–838 Occurrence Handle1:CAS:528:DC%2BD2MXlvVWisbw%3D Occurrence Handle10.1369/jhc.4A6577.2005 A Matsuno J Itoh S Takekoshi et al. (2003) ArticleTitleDynamics of subcellular organelles, growth hormone, rab3B, SNAP-25, and syntaxin in rat pituitary cells caused by growth hormone releasing hormone and somatostatin Microsc Res Tech 62 232–239 Occurrence Handle14506689 Occurrence Handle1:CAS:528:DC%2BD3sXosF2mtbs%3D Occurrence Handle10.1002/jemt.10364 A Matsuno J Itoh S Takekoshi et al. (2003) ArticleTitleFunctional and morphological analyses of rab proteins and the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) system in the secretion of pituitary hormones Acta Histochem Cytochem 36 501–506 Occurrence Handle1:CAS:528:DC%2BD2cXhtlGntrs%3D Occurrence Handle10.1267/ahc.36.501 A Matsuno A Mizutani J Itoh et al. (2005) ArticleTitleEstablishment of stable GH3 cell line expressing enhanced yellow fluorescein protein (EYFP) – growth hormone (GH) fusion protein J Histochem Cytochem 53 1177–1180 Occurrence Handle15923356 Occurrence Handle1:CAS:528:DC%2BD2MXhtVersbrK Occurrence Handle10.1369/jhc.5B6708.2005 TD Lacoste X Michalet F Pinaud et al. (2000) ArticleTitleUltrahigh-resolution multicolor colocalization of single fluorescent probes Proc Natl Acad Sci USA 97 9461–9466 Occurrence Handle10931959 Occurrence Handle1:CAS:528:DC%2BD3cXmtVens7k%3D Occurrence Handle10.1073/pnas.170286097 X Michalet F Pinaud TD Lacoste et al. (2001) ArticleTitleProperties of fluorescent semiconductor nanocrystals and their application to biological labeling Single Mol 4 261–276 Occurrence Handle10.1002/1438-5171(200112)2:4<261::AID-SIMO261>3.0.CO;2-P RY Tsien (1998) ArticleTitleThe green fluorescent protein Annu Rev Biochem 67 509–544 Occurrence Handle9759496 Occurrence Handle1:CAS:528:DyaK1cXlsFOmsb8%3D Occurrence Handle10.1146/annurev.biochem.67.1.509 C Magoulas L McGuinness N Balthasar et al. (2000) ArticleTitleA secreted fluorescent reporter targeted to pituitary growth hormone cells in transgenic mice Endocrinology 141 4681–4689 Occurrence Handle11108283 Occurrence Handle1:CAS:528:DC%2BD3cXosVSqtro%3D Occurrence Handle10.1210/en.141.12.4681