Amperometric Alcohol-Sensing Electrode Based on a Polyion Complex Membrane Containing Alcohol Oxidase
Tóm tắt
An amperometric alcohol-sensing electrode was prepared by immobilizing alcohol oxidase (AOx) in a polyion complex membrane. Three kinds of aqueous solutions (poly(4-styrenesulfonate)-, AOx- and poly-L-lysine-containing solutions) were successively placed on the surface of a glassy carbon electrode and the electrode was allowed to dry. The anodic current (at 1 V vs. Ag/AgCl) of the enzyme electrode increased immediately after the addition of alcohols (e.g., ethanol), and reached a steady state within 5 s. A linear response to ethanol was observed up to 0.4 mM with a detection limit of 0.5 μM. The polyion complex membrane showed permselectivity based on the solute size with a molecule weight cut-off of ca. 100, which was very effective in suppressing the electrochemical interference by L-ascorbic acid and uric acid. The enzyme electrode was applied to the determination of ethanol in alcoholic drinks and soy sources. The electrode could be used for two weeks.
Tài liệu tham khảo
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