Aminoalkylsilane-treated glass slides as support forin situ hybridization of keratin cDNAs to frozen tissue sections under varying fixation and pretreatment conditions

Martin Rentrop1, Bernhard Knapp1, Hermelita Winter1, Jürgen Schweizer1
1Institute of Experimental Pathology, German Cancer Research Center, Im Neuenheimer Feld 280, 6900, Heidelberg, Germany

Tóm tắt

Từ khóa


Tài liệu tham khảo

ANGERER, L. M. & ANGERER, R. C. (1981) Detection of polyA+RNA in sea urchin eggs and embryos by quantitativein situ hybridization.Nucleic Acids Res. 9 2819–39.

BONNER, J. J. & PARDUE, M. L. (1976) Ecdysone-stimulated RNA synthesis in imaginal discs ofDrosophila melanogaster. Assay byin situ hybridization.Chromosoma 58 87–99.

BRAHIC, M. & HAASE, A. T. (1978) Detection of viral sequences of low reiteration frequency byin situ hybridization.Proc. Natl. Acad. Sci. USA 75 6125–9.

BRAHIC, M., HAASE, A. T. & CASH, E. (1984) Simultaneousin situ detection of viral RNA and antigens.Proc. Natl. Acad. Sci. USA 81 5445–8.

CAMERON, I. L. (1966) Cell proliferation, migration and specialization in the epithelium of the mouse tongue.J. Expl. Zool. 163 271–84.

CRAWFORD, R. J., PENSCHOW, J. D., NIALL, H. D. & COGHLAN, I. P. (1985) Mouse prepro-epidermal growth factor synthesis by the kidney and other tissues.Nature 313 228–31.

DENHARDT, D. (1966) A membrane filter technique for the detection of complementary DNA.Biochem. Biophys. Res. Commun. 23 641–6.

GALL, J. & PARDUE, M. L. (1969) Formation and detection of RNA—DNA hybrid molecules in cytological preparationsProc. Natl. Acad. Sci. USA 63 378–83.

GALL, J. & PARDUE, M. L. (1971) Nucleic acid hybridization in cytological preparations. InMethods in Enzymology, Vol. XXI (edited by GROSSMAN, L. and MOLDAVE, K.), pp. 470–80. New York: Academic Press.

GIZANG-GINSBERG, E. & WOLGEMUTH, D. J. (1985) Localization of mRNAs in mouse testes byin situ hybridization: distribution ofα-tubulin and developmental stage specificity of pro-opiomelanocortin transcripts.Devel. Biol. 111, 293–305.

GUELIN, M., KEJZLAROVA-LEPESANT, J. & LEPESANT, J. A. (1985)In situ hybridization: a routine method for parallel localization of DNA sequences and of their transcripts in consecutive paraffin sections with the use of [3H]-labelled nick translated cloned DNA probes.Biol. Cell. 53, 1–11.

HAASE, A., BRAHIC, M., STOWRING, L. & BLUM, H. (1984) Detection of viral nucleic acids byin situ hybridization. InMethods in Virology, Vol. VII (edited by MARAMOROSCH, K. and KOPROWSKI, H.), pp. 189–226. New York: Academic Press.

HAFEN, E., LEVINE, M., GARBER, R. L. & GEHRING, W. I. (1983) An improvedin situ hybridization method for the detection of cellular RNAs inDrosphila tissue sections and its application for localizing transcripts of the homeotic Antennapedia gene.EMBO J. 2, 617–23.

KNAPP, B., RENTROP, M., SCHWEIZER, J. & WINTER, H. (1986) Nonepidermal members of the keratin multigene family: cDNA sequences andin situ localization of the mRNA.Nucleic Acids Res. 14, 751–63.

LAWRENCE, J. B. & SINGER, R. H. (1985) Quantitative analysis ofin situ hybridization methods for the detection of actin gene expression.Nucleic Acids Res. 13 1777–99.

POTTEN, C. S., SCHOFIELD, R. & LAJTHA, L. G. (1979) A comparison of cell replacement in bone marrow, testis, and three regions of surface epithelium.Biochim. Biophys. Acta 560, 281–99.

RIGBY, P. W. J., DIECKMANN, M., RHODES, C. & BERG, P. (1977) Labelling deoxyribonucleic acid to high specific activityin vitro by nick translation with DNA polymerase I.J. Mol. Biol. 113 237–51.

ROBINSON, P. J., DUNHILL, P. & LILLY, M. D. (1971) Porous glass as a solid support for immobilization or affinity chromotography of enzymes.Biochim. Biophys. Acta 242 659–61.

SCHWEIZER, J., KINJO, M., FÜRSTENBERGER, G. & WINTER, H. (1984) Sequential expression of mRNA-encoded keratin sets in neonatal mouse epidermis: basal cells with properties of terminally differentiating cells.Cell 37 159–70.

THOMAS, P. (1980) Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proc. Natl. Acad. Sci. USA 77 5201–5.

VAN PROOIJEN-KNEGT, A. C., RAAP, A. K., VAN DER BURG, M. J. M., VROLIJK, J. & VAN DER PLOEG, M. (1982) Spreading and staining of human metaphase chromosomes on aminoalkylsilane-treated glass slides.Histochem. J. 14, 333–44.