Actin localization in nuclei of two-cell mouse embryos

Cell and Tissue Biology - Tập 3 - Trang 417-422 - 2009
N. A. Bogolyubova1, I. O. Bogolyubova1
1Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia

Tóm tắt

In this study, preimplantation mouse embryos were used as a new model for the study of actin distribution in nuclei and the identification of functional forms of nuclear actin. The combination of the direct detection of actin by fluorescent-conjugated phalloidin and DNase I with indirect immunofluorecence was applied as an integrated approach to studying the localization of actin in nuclei of two-cell mouse embryos. Aggregates of monomeric actin and two oligomeric forms of actin were revealed in nuclei. Each of these forms demonstrated their own pattern of distribution. Oligomeric actin recognized by antibodies to the actin C-terminal domain was associated with condensed chromatin, as well as with metaphase chromosomes and chromatin of the second polar body. Monomeric actin and another oligomeric form recognized by antibodies to actin N-terminal domain were revealed in the area of dispersed chromatin localization.

Tài liệu tham khảo

Aguda, A.H., Burtnick, L.D., and Robinson, R.C., The State of the Filament, EMBO Rep., 2005, vol. 6, pp. 220–226.

Baranov, V.S. and Kuznetsova, T.V., Tsitogenetika embrional'nogo razvitiya cheloveka (Cytogenetics of Human Embryonic Development), St. Petersburg: Izd. N-L.

Bettinger, B.T., Gilbert, D.M., and Amberg, D.C., Actin Up in the Nucleus, Nat. Rev. Mol. Cell. Biol., 2004, vol. 5, pp. 410–415.

Blessing, C.A., Urginova, G.T., and Goodson, H.V., Actin and ARPs: Action in the Nucleus, Trends Cell. Biol., 2004, vol. 14, pp. 435–442.

Bogolyubova, I.O. and Parfenov, V.N., Distribution of RNA Polymerase II in Nuclei of Early Murine Embryos, Tsitologiya, 2002, vol. 44, no. 2, pp. 175–180.

Bogolyubova, I.O. and Parfenov, V.N., Pre-mRNA Splicing Factors in Nuclei of Two-Cells Murine Embryos, Tsitologiya, 2000, vol. 42, no. 3, pp. 884–890.

Bohnsack, M.T., Stuven, T., Kuhn, C., Cordes, V.C., and Golrich, D., A Selective Block of Nuclear Actin Export Stabilizes the Giant Nuclei of Xenopus Oocytes, Nat. Cell. Biol., 2006, vol. 8, pp. 257–263.

Clark, T.G. and Rosenbaum, J.L., An Actin Filament Matrix in Hand-Isolated Nuclei of X. laevis Oocytes, Cell, 1979 vol. 18, pp. 1101–1108.

De Lanerolle, P., Johnson, T., and Hofmann, W., Actin and Myosin I in the Nucleus: What Next?, Nat. Struct. Mol. Biol., 2005, vol. 12, pp. 742–746.

Fakan, S. and Odartchenko, N., Ultrastructural Organization of the Cell Nucleus in Early Mouse Embryos, Biol. Cell. (Biologie Cellulaire), 1980, vol. 37, pp. 211–218.

Funaki, K., Katsumoto, T., and Iino, A., Immunocytochemical Localization of Actin in the Nucleolus of Rat Oocytes, Biol. Cell., 1995, vol. 84, pp. 139–146.

Gall, J.G., Exporting Actin, Nat. Cell Biol., 2006, vol. 8, pp. 205–207.

Geuskens, M. and Alexandre, H., Ultrastructural and Autoradiographic Studies of Nucleolar Development and rDNA Transcription in Preimplantation Mouse Embryos, Cell. Differ., 1984, vol. 14, 125–134.

Gonsior, S.M., Platz, S., Buchmeier, S., Scheer, U., Jockusch, B.M., and Hinssen, H., Conformation Difference between Nuclear and Cytoplasmic Actin as Detected by a Monoclonal Antibody, J. Cell. Sci., 1999, vol. 112, pp. 797–809.

Johnson, N., Krebs, M., Boudreau, R., Giorgi, G, et al., Actin-Filled Nuclear Invaginations Indicate Degree of Cell De-differentiation, Differentiation, 2003, vol. 71, pp. 414–424.

Krauss, S.W., Heald, R., Lee, G., Nunomura, W., Gimm, J.A., Mohandas, N., and Chasis, J.A., Two Distinct Domains of Protein 4.1 Critical for Assembly of Functional Nuclei in Vitro, J. Biol. Chem., 2002, vol. 277, pp. 44339–44346.

Latham, K.E., Mechanisms and Control of Embryonic Genome Activation in Mammalian Embryos, Int. Rev. Cytol., 1999, vol. 193, pp. 71–124.

Ma, J., Svoboda, P., Shultz, R.M., and Stein, P., Regulation of Zygotic Gene Activation in the Preimplantation Mouse Embryo: Global Activation and Repression of Gene Expression, Biol. Reprod., 2001, vol. 64, pp. 1713–1721.

Milankov, K. and De Boni, U., Cytochemical Localization of Actin and Myosin Aggregates in Interphase Nuclei in Situ, Exp. Cell. Res., 1993, vol. 209, pp. 189–195.

Miralles, F. and Visa, N., Actin in Transcription and Transcription Regulation, Curr. Opin. Cell Biol., 2006, vol. 18, pp. 261–266.

Nakayasu, H. and Ueda, K., Association of Actin with the Nuclear Matrix from Bovine Lymphocytes, Exp. Cell. Res., 1983, vol. 143, pp. 55–62.

Nguyen, E., Besombes, D., and Debey, P., Immunofluorescent Localization of Actin in Relation to Transcription Sites in Mouse Pronuclei, Mol. Reprod. Dev., 1998, vol. 50, pp. 263–272.

Ohta, Y., Nishida, E., Sakai, H., and Miyamoto, E., Dephosphorylation of Cofilin Accompanies Heat Shock Induced Nuclear Accumulation of Cofilin, J. Biol. Chem., 1989, vol. 264, pp. 16143–16148.

Olave, J.A., Reck-Peterson, S.L., and Crabtree, G.R., Nuclear Actin and Actin-Related Proteins in Chromatin Remodeling, Ann. Rev. Biochem., 2002, vol. 71, pp. 755–781.

Papuulas, O., Beck, S.J., Moseley, S.L., McCallum, C.M., Sarte, M., Shearn, A., and Amkun, J.W., TheDrosophila Trithorax Group Proteins BRM, ASH1 and ASH2 Are Subunits of Distinct Protein Complexes, Development, 1998, vol. 125, pp. 3955–3966.

Parfenov, V.N. and Galaktionov, K.I., Intranuclear Actin Microfilaments in the Oocytes of the Common Frog, Tsitologiya, 1987, vol. 29, no. 2, pp. 142–149.

Patkin, E.L., Study of Structural Heterochromatin at Initial Stages of Mouse Embryo Development, Ontogenez, 1980, vol. 11, no. 1, pp. 49–55.

Pedersen, T., As Functional Nuclear Actin Comes into View, Is It Globular, Filamentous, or Both?, J. Cell. Biol., 2008, vol. 180, pp. 1061–1064.

Rawe, V.Y., Payne, C., Schatten, G., Profilin and Actin-Related Proteins Regulate Microfilament Dynamics During Early Mammalian Embryogenesis, Human Reprod., 2006, vol. 21, pp. 1143–1153.

Shen, X., Mizuguchi, G., Hamiche, A., and Wu, C., A Chromatin Remodeling Complex Involved in Transcription and RNA Processing, Nature, 2000, vol. 406, pp. 541–544.

Shumaker, D.K., Kuczmarski, E.R., and Goldman, R.D., The Nucleoskeleton: Lamins and Actin Are Major Players in Essential Nuclear Functions, Curr. Opin. Cell. Biol., 2003, vol. 15, pp. 358–366.

Steinmetz, M.O., Stoffler, D, and Hoenger, A., Actin: From Cell Biology to Atomic Detail, J. Struct. Biol., 1997, vol. 119, pp. 295–320.

Zatsepina, O., Baly, C., Chebrout, M., and Debey, P., The Step-Wise Assembly of a Functional Nucleolus in Preimplantation Mouse Embryos Involves the Cajal (Coiled) Body, Dev. Biol., 2003, vol. 253, 66–83

Zhao, K., Wang, W, Rando, O.J., Xue, Y., Swiderek, K., Kuo, A., and Cradtree, G.R., Rapid and Phosphoinositol-Dependent Binding of the SWI/SNF-like BAF Complex to Chromatin after T-lymphocyte Receptor Signalling, Cell, 1998, vol. 95, pp. 625–636.

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Thông tin xuất bản

Cell and Tissue Biology

Tập 3

417-422

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