A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei

Hillen, 1994, Mechanisms underlying expression of TN10 -encoded tetracycline resistance, Annu. Rev. Microbiol., 48, 345, 10.1146/annurev.mi.48.100194.002021

Wirtz, 1995, Inducible gene expression in trypanosomes mediated by a prokaryotic repressor, Science, 268, 1179, 10.1126/science.7761835

Biebinger, 1997, Vectors for inducible expression of toxic gene products in bloodstream and procyclic Trypanosoma brucei, Mol. Biochem. Parasitol., 85, 99, 10.1016/S0166-6851(96)02815-0

Biebinger, 1996, The PARP promoter of Trypanosoma brucei is developmentally regulated in a chromosomal context, Nucl. Acids Res., 24, 1202, 10.1093/nar/24.7.1202

Wirtz, 1998, Regulated processive transcription of chromatin by T7 RNA polymerase in Trypanosoma brucei, Nucl. Acids Res., 26, 4626, 10.1093/nar/26.20.4626

Wirtz, 1994, Gene expression mediated by bacteriophage T3 and T7 RNA polymerases in transgenic trypanosomes, Nucl. Acids Res., 22, 3887, 10.1093/nar/22.19.3887

Häusler, 1996, Import of a DHFR hybrid protein into glycosomes in vivo is not inhibited by the folate-analogue aminopterin, J. Cell Biol., 132, 311, 10.1083/jcb.132.3.311

Cooper, 1993, Deletion of an immunodominant Trypanosoma cruzi surface glycoprotein disrupts flagellum-cell adhesion, J. Cell. Biol., 122, 149, 10.1083/jcb.122.1.149

Brun, 1979, Cultivation and in vitro cloning of procyclic culture forms of Trypanosoma brucei in a semi-defined medium, Acta Trop., 36, 289

van den Hoff, 1992, Electroporation in 'intracellular' buffer increases cell survival, Nucl. Acids Res., 20, 2902, 10.1093/nar/20.11.2902

Rudenko, 1994, VSG gene expression site control in insect form Trypanosoma brucei, EMBO J., 13, 5470, 10.1002/j.1460-2075.1994.tb06882.x

Urmenyi, 1995, PARP promoter-mediated activation of a VSG expression site promoter in insect form Trypanosoma brucei, Nucl. Acids Res., 23, 1010, 10.1093/nar/23.6.1010

Pays, 1990, Trypanosoma brucei: constitutive activity of the VSG and procyclin gene promoters, EMBO J., 9, 3145, 10.1002/j.1460-2075.1990.tb07512.x

Hug, 1993, A possible role for the 3′-untranslated region in developmental regulation in Trypanosoma brucei, Mol. Biochem. Parasitol., 61, 87, 10.1016/0166-6851(93)90161-P

Nguyen, 1989, Protein denaturation during heat shock and related stress. Escherichia coli beta-galactosidase and Photinus pyralis luciferase inactivation in mouse cells, J. Biol. Chem., 264, 10487, 10.1016/S0021-9258(18)81647-7

Schroder, 1993, DnaK, DnaJ and GrpE form a cellular chaperone machinery capable of repairing heat-induced protein damage, EMBO J., 12, 4137, 10.1002/j.1460-2075.1993.tb06097.x

Dubendorff, 1991, Creation of a T7 autogene. Cloning and expression of the gene for bacteriophage T7 RNA polymerase under control of its cognate promoter, J. Mol. Biol., 219, 61, 10.1016/0022-2836(91)90857-3

Overath, 1986, The effect of citrate/cis-aconitate on oxidative metabolism during transformation of Trypanosoma brucei, Eur. J. Biochem., 160, 175, 10.1111/j.1432-1033.1986.tb09955.x

Treumann, 1997, Structural characterisation of two forms of procyclic acidic repetitive protein expressed by procyclic forms of Trypanosoma brucei, J. Mol. Biol., 269, 529, 10.1006/jmbi.1997.1066

Butikofer, 1997, GPEET procyclin is the major surface protein of procyclic culture forms of Trypanosoma brucei brucei strain 427, Biochem. J., 326, 415, 10.1042/bj3260415

Carruthers, 1993, DNA-mediated transformation of bloodstream-form Trypanosoma brucei, Nucl. Acids Res., 21, 2537, 10.1093/nar/21.10.2537