A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback
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Akam ME (1983) The location of Ultrabithorax transcripts in Drosophila tissue sections. EMBO J 2:2075?2084
Akam ME (1987) The molecular basis for metameric pattern in the Drosophila embryo. Development 101:1?22
Bender M, Horikami S, Cribbs D, Kaufman TC (1988) Identification and expression of the gap segmentation gene hunchback in Drosophila melanogaster. Dev Genet 9:715?732
Driever W, Nüsslein-Volhard C (1988) A gradient of bicoid protein in Drosophila embryos. Cell 54:83?93
Driever W, Nüsslein-Volhard C (1989) The bicoid protein is a positive regulator of hunchback transcription in the early Drosophila embryo. Nature 337:138?143
Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6?13
Foe VE, Alberts BM (1983) Studies of nuclear and cytoplasmic behavior during the five mitotic cycles that precede gastrulation in Drosophila embryogenesis. J Cell Sci 61:31?70
Frohnhöfer HG, Nüsslein-Volhard C (1986) Organization of anterior pattern in the Drosophila embryo by the maternal gene bicoid. Nature 324:120?125
Hafen E, Levine M, Garber RL, Gehring WJ (1983) An improved in situ hybridization method for the detection of cellular RNAs in Drosophila tissue sections and its application for localizing transcripts of the homoeotic Antennapedia gene complex. EMBO J 12:617?623
Hülskamp M, Schröder C, Pfeifle C, Jäckle H, Tautz D (1989) Posterior segmentation of the Drosophila embryo in the absence of a maternal posterior organizer gene. Nature 338:629?632
Ingham PW (1988) The molecular genetics of embryonic pattern formation in Drosophila. Nature 335:25?34
Kuroiwa A, Hafen E, Gehring WJ (1984) Cloning and transcriptional analysis of the segmentation gene fushi tarazu of Drosophila. Cell 37:825?831
Lawrence PA, Johnston P, Morata G (1986) Methods of marking cells. In: Roberts DB (ed) Drosophila, a practical approach, IRL Press, Oxford, pp 229?242
Lehmann R, Nüsslein-Volhard C (1984) Hunchback, a gene required for segmentation of an anterior and posterior region of the embryo. Dev Biol 119:402?417
Macdonald PM, Struhl G (1986) A molecular gradient in early Drosophila embryos and its role in specifying the body pattern. Nature 324:537?545
Nauber U, Pankratz M, Kienlin A, Seifert E, Klemm U, Jäckle H (1988) Abdominal segmentation of the Drosophila embryo requires a hormone receptor like protein encoded by the gap gene knirps. Nature 336:489?492
Nüsslein-Volhard C, Wieschaus E (1980) Mutations affecting segment number and polarity in Drosophila. Nature 287:795?801
Nüsslein-Vollhard C, Frohnhöfer HG, Lehmann R (1987) Determination of Anteroposterior polarity in Drosophila. Science 238:1675?1681
Preiss A, Rosenberg UB, Kienlin A, Seifert E, Jäckle H (1985) Molecular genetics of Krüppel, a gene required for segmentation of the Drosophila embryo. Nature 313:27?32
Schröder C, Tautz D, Seifert E, Jäckle H (1988) Differential regulation of the two transcripts from the Drosophila gap segmentation gene hunchback. EMBO J 7:2881?2887
Tautz D (1988) Regulation of the Drosophila segmentation gene hunchback by two maternal morphogenetic centres. Nature 332:281?284
Tautz D, Renz M (1983) An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels. Anal Biochem 132:14?19
Tautz D, Lehmann R, Schnürch H, Schuh R, Seifert E, Kienlin A, Jones K, Jäckle H (1987) Fingerprotein of novel structure encoded by hunchback, a second member of the gap class of Drosophila segmentation genes. Nature 327:383?389
Weiner AJ, Scott MP, Kaufmann TC (1984) A molecular analysis of fushi tarazu, a gene in Drosophila melanogaster that encodes a product affecting embryonic segment number and cell fate. Cell 37:843?851
Wieschaus E, Nüsslein-Volhard C (1986) Looking at embryos. In: Roberts DB (ed) Drosophila, a practical approach. IRL Press, Oxford, pp 199?228