A mutation that blocks exopolysaccharide synthesis prevents nodulation of peas by Rhizobium leguminosarum but not of beans by R. phaseoli and is corrected by cloned DNA from Rhizobium or the phytopathogen Xanthomonas

Springer Science and Business Media LLC - Tập 203 Số 2 - Trang 320-323 - 1986
Dulal Borthakur1, C. E. Barber1, J. W. Lamb1, Michael J. Daniels1, J. A. Downie, Andrew Johnston1
1John Innes Institute, Norwich, UK

Tóm tắt

Từ khóa


Tài liệu tham khảo

Beringer JE (1974) R factor transfer in Rhizobium leguminosarum. J Gen Microbiol 84:188–198

Chakravorty AK, Zurkowski W, Shine J, Rolfe BG (1982) Symbiotic nitrogen fixation: molecular cloning of Rhizobium genes involved in exopolysaccharide synthesis and effective nodulation. J Mol Appl Genet 1:585–596

Daniels MJ, Barber CE, Turner PC, Sawzyc MK, Byrde RJW, Fielding AH (1984) Cloning of genes involved in pathogenicity of Xanthomonas campestris pv. campestris using the broad host range cosmid pLAFR1. EMBO J 3:3323–3328

Downie JA, Hombrecher G, Ma Q-S, Knight CD, Wells B, Johnston AWB (1983a) Cloned nodulation genes of Rhizobium leguminosarum determine host range specificity. Mol Gen genet 190:359–365

Downie JA, Ma Q-S, Knight CD, Hombrecher G, Johnston AWB (1983b) Cloning of the symbiotic region of Rhizobium leguminosarum: the nodulation genes are between the nitrogenase genes and a nifA-like gene. EMBO J 2:947–952

Figurski DH, Helinski DR (1979) Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci USA 76:1648–1652

Friedman AM, Long SR, Brown SE, Buikema WJ, Ausubel FM (1982) Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. Gene 18:289–296

Hewitt EJ, Smith TA (1975) Plant mineral nutrition. English Universities Press London, pp 31–36

Hombrecher G, Brewin NJ, Johnston AWB (1981) Linkage of genes for nitrogenase and nodulation ability on plasmids in Rhizobium leguminosarum and R. phaseoli. Mol Gen Genet 182:133–136

Jorgensen RA, Rothstein SJ, Reznikoff WS (1979) A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance. Mol Gen Genet 177:65–72

Leigh JA, Singer ER, Waker GC (1985) Exopolysaccharide-deficient mutants of Rhizobium meliloti that form ineffective nodules. Proc Natl Acad Sci USA 82:6231–6235

Merrick M, Filser M, Kennedy C, Dixon R (1978) Polarity of mutations induced by insertion of transposons Tn5, Tn7 and Tn10 into the nif gene cluster of Klebsiella pneumoniae. Mol Gen Genet 165:103–111

Lamb JW, Hombrecher G, Johnston AWB (1982) Plasmid-determined nodulation and nitrogen fixation abilities in Rhizobium phaseoli. Mol Gen Genet 186:449–452

Lamb JW, Downie JA, Johnston AWB (1985) Cloning of the nodulation (nod) genes of Rhizobium phaseoli and their homology to R. leguminosarum nod genes. Gene 34:235–241

Ruvkun GB, Ausubel FM (1981) A general method for site-directed mutagenesis in procaryotes. Nature 289:85–88

Sanders R, Raleigh E, Signer E (1981) Lack of correlation between extracellular polysaccharide and nodulation ability in Rhizobium. Nature 292:148–149

Sandford PA, Baird J (1983) In: Aspinall GO (Ed) The polysaccharides. Vol. 2. Academic Press, New York, pp 411–490

Sutherland IW (1985) Biosynthesis and composition of Gram negative bacterial extracellular and wall polysaccharides. Ann Rev Microbiol 39:243–270

Wood WB (1966) Host specificity of DNA produced by E. coli bacterial mutations affecting the restriction and modification of DNA. J Mol Biol 16:118–133

Young JPW (1985) Rhizobium population genetics: enzyme polymophism in isolates from peas, clover, beans and lucerne grown at the same site. J Gen Microbiol 131:2399–2408