A method to size DNA fragments from 50 to 800 bp on a DNA analyser

Wiley - Tập 5 Số 4 - Trang 969-970 - 2005
Peter G. Avis1, Kevin A. Feldheim2
1Department of Botany, The Field Museum,
2Pritzker Molecular Laboratory, The Field Museum, 1400 S. Lake Shore Drive, Chicago, IL 60605, USA

Tóm tắt

AbstractWe present a method to size DNA fragments from 50 to 800 bp on a DNA analyser using an alternative size standard (X‐Rhodamine MapMarker 1000) run on a capillary electrophoresis system (Applied Biosystem's 3730 DNA Analyser). This method avoids several limitations imposed by manufacturer‐developed size standards, instruments and software and offers a more robust approach to conducting DNA size fragment analyses.

Từ khóa


Tài liệu tham khảo

Delmotte F, 2001, Microsatellite allele sizing: difference between automated capillary electrophoresis and manual technique, BioTechniques, 31, 810

10.2144/04373BM02

10.1046/j.1469-8137.2002.00535.x

10.1002/(SICI)1522-2683(19990601)20:7<1518::AID-ELPS1518>3.0.CO;2-5

10.1002/1522-2683(200108)22:13<2670::AID-ELPS2670>3.0.CO;2-1

10.1111/j.1471-8286.2004.00763.x

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Thông tin xuất bản

Wiley

Tập 5 Số 4

969-970

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