A method to size DNA fragments from 50 to 800 bp on a DNA analyser
Tóm tắt
We present a method to size DNA fragments from 50 to 800 bp on a DNA analyser using an alternative size standard (X‐Rhodamine MapMarker 1000) run on a capillary electrophoresis system (Applied Biosystem's 3730 DNA Analyser). This method avoids several limitations imposed by manufacturer‐developed size standards, instruments and software and offers a more robust approach to conducting DNA size fragment analyses.
Từ khóa
Tài liệu tham khảo
Delmotte F, 2001, Microsatellite allele sizing: difference between automated capillary electrophoresis and manual technique, BioTechniques, 31, 810