A conserved coccidian gene is involved in Toxoplasma sensitivity to the anti-apicomplexan compound, tartrolon E

Altschul, 1990, Basic local alignment search tool, J. Mol. Biol., 215, 403, 10.1016/S0022-2836(05)80360-2 Behnke, 2008, The transcription of bradyzoite genes in Toxoplasma gondii is controlled by autonomous promoter elements, Mol. Microbiol., 68, 1502, 10.1111/j.1365-2958.2008.06249.x Behnke, 2014, Toxoplasma gondii merozoite gene expression analysis with comparison to the life cycle discloses a unique expression state during enteric development, BMC Genom., 15, 10.1186/1471-2164-15-350 Blom, 1999, Sequence and structure-based prediction of eukaryotic protein phosphorylation sites, J. Mol. Biol., 294, 1351, 10.1006/jmbi.1999.3310 Blom, 2004, Prediction of post-translational glycosylation and phosphorylation of proteins from the amino acid sequence, Proteomics, 4, 1633, 10.1002/pmic.200300771 Caballero, 2016, Identification of new palmitoylated proteins in Toxoplasma gondii, Biochim. Biophys. Acta Protein Proteonomics, 1864, 400, 10.1016/j.bbapap.2016.01.010 Couzinet, 2000, In vitro activity of the polyether ionophorous antibiotic monensin against the cyst form of Toxoplasma gondii, Parasitology, 121, 359, 10.1017/S0031182099006605 Delorme-Walker, 2012, Toxofilin upregulates the host cortical actin cytoskeleton dynamics, facilitating Toxoplasma invasion, J. Cell Sci., 125, 4333, 10.1242/jcs.103648 Dybas, 2008, Computational analysis and experimental validation of gene predictions in Toxoplasma gondii, PloS One, 3, e3899, 10.1371/journal.pone.0003899 Elshahawi, 2013, Boronated tartrolon antibiotic produced by symbiotic cellulose-degrading bacteria in shipworm gills, Proc. Natl. Acad. Sci. U.S.A., 110, E295, 10.1073/pnas.1213892110 Foe, 2015, Global analysis of palmitoylated proteins in Toxoplasma gondii, Cell Host Microbe, 18, 501, 10.1016/j.chom.2015.09.006 Fritz, 2012, Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts, PloS One, 7, 10.1371/journal.pone.0029998 Gajria, 2008, ToxoDB: an integrated Toxoplasma gondii database resource, Nucleic Acids Res., 36 Garrison, 2009, Disruption of a mitochondrial MutS DNA repair enzyme homologue confers drug resistance in the parasite Toxoplasma gondii, Mol. Microbiol., 72, 425, 10.1111/j.1365-2958.2009.06655.x Gouw, 2018, The eukaryotic linear motif resource - 2018 update, Nucleic Acids Res., 46, D428, 10.1093/nar/gkx1077 Gumila, 1997, Characterization of the potent in vitro and in vivo antimalarial activities of ionophore compounds, Antimicrob. Agents Chemother., 41, 523, 10.1128/AAC.41.3.523 Horton, 2007, WoLF PSORT: protein localization predictor, Nucleic Acids Res., 35, 585, 10.1093/nar/gkm259 Howe, 2019, Ensembl Genomes 2020-enabling non-vertebrate genomic research, Nucleic Acids Res., 48, 689, 10.1093/nar/gkz890 Irschik, 1995, The tartrolons, new boron-containing antibiotics from a myxobacterium, Sorangium cellulosum, J. Antibiot. (Tokyo), 48, 26, 10.7164/antibiotics.48.26 Kevin Ii, 2009, Polyether ionophores: broad-spectrum and promising biologically active molecules for the control of drug-resistant bacteria and parasites, Expet Opin. Drug Discov., 4, 109, 10.1517/17460440802661443 Krogh, 2001, Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes, J. Mol. Biol., 305, 567, 10.1006/jmbi.2000.4315 Lavine, 2011, The antibiotic monensin causes cell cycle disruption of Toxoplasma gondii mediated through the DNA repair enzyme TgMSH-1, Antimicrob. Agents Chemother., 55, 745, 10.1128/AAC.01092-10 Lewer, 2003, Tartrolone C: a novel insecticidal macrodiolide produced by Streptomyces sp. CP1130, J. Nat. Prod., 66, 143, 10.1021/np020451s Livak, 2001, Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method, Methods, 25, 402, 10.1006/meth.2001.1262 Marchler-Bauer, 2015, CDD: NCBI's conserved domain database, Nucleic Acids Res., 43, D222, 10.1093/nar/gku1221 Mayoral, 2020, Toxoplasma gondii: bradyzoite differentiation in vitro and in vivo, 269, 10.1007/978-1-4939-9857-9_15 McDonald, 1990, In vitro cultivation of Cryptosporidium parvum and screening for anticryptosporidial drugs, Antimicrob. Agents Chemother., 34, 1498, 10.1128/AAC.34.8.1498 Medina-Acosta, 1993, Rapid isolation of DNA from trypanosomatid protozoa using a simple “mini-prep” procedure, Mol. Biochem. Parasitol., 59, 327, 10.1016/0166-6851(93)90231-L Mineo, 1994, Attachment of Toxoplasma gondii to host cells involves major surface protein, SAG-1 (P-30), Exp. Parasitol., 79, 11, 10.1006/expr.1994.1054 Mineo, 1993, Antibodies to Toxoplasma gondii major surface protein (SAG-1, P30) inhibit infection of host cells and are produced in murine intestine after peroral infection, J. Immunol., 150, 3951, 10.4049/jimmunol.150.9.3951 Moreira, 2016, Boromycin kills mycobacterial persisters without detectable resistance, Front. Microbiol., 7, 10.3389/fmicb.2016.00199 NCBI Resource Coordinators, 2016, Database resources of the national center for Biotechnology information, Nucleic Acids Res., 44, D7, 10.1093/nar/gkv1290 O'Connor, 2020, A symbiotic bacterium of shipworms produces a compound with broad spectrum anti-apicomplexan activity, PLoS Pathog., 16 Pache, 1969, Metabolic products of microorganisms. 77. Studies on the mechanism of action of boromycin, Arch. für Mikrobiol., 67, 156, 10.1007/BF00409681 Pfefferkorn, 1976, Toxoplasma gondii: isolation and preliminary characterization of temperature-sensitive mutants, Exp. Parasitol., 39, 365, 10.1016/0014-4894(76)90040-0 Poupel, 2000, Toxofilin, a novel actin-binding protein from Toxoplasma gondii, sequesters actin monomers and caps actin filaments, Mol. Biol. Cell, 11, 355, 10.1091/mbc.11.1.355 Ren, 2008, CSS-Palm 2.0: an updated software for palmitoylation sites prediction, Protein Eng. Des. Sel., 21, 639, 10.1093/protein/gzn039 Roos, 1995, Molecular tools for genetic dissection of the Protozoan parasite Toxoplasma gondii, Methods Cell Biol., 45, 27, 10.1016/S0091-679X(08)61845-2 Sambrook, 2006, Standard ethanol precipitation of DNA in microcentrifuge tubes, CSH Protoc., 2006 Schummer, 1994, Antibiotics from gliding bacteria, LVII. Tartrolons: new boron‐containing macrodiolides from Sorangium cellulosum, Liebigs Ann. Chem., 283, 10.1002/jlac.199419940310 Shen, 2014, Efficient gene disruption in diverse strains of Toxoplasma gondii Using CRISPR/CAS9, mBio, 5, 10.1128/mBio.01114-14 Sidik, 2016, A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes, Cell, 166, 1423, 10.1016/j.cell.2016.08.019 Soldati, 1993, Transient transfection and expression in the obligate intracellular parasite Toxoplasma gondii, Science (80-. ), 260, 349, 10.1126/science.8469986 Stothard, 2000, The sequence manipulation suite: JavaScript programs for analyzing and formatting protein and DNA sequences, Biotechniques, 28, 10.2144/00286ir01 Surup, 2018, Activation of the NLRP3 inflammasome by hyaboron, a new asymmetric boron-containing macrodiolide from the myxobacterium Hyalangium minutum, ACS Chem. Biol., 13, 2981, 10.1021/acschembio.8b00659 Tarleton, 2015, EuPaGDT: a web tool tailored to design CRISPR guide RNAs for eukaryotic pathogens, Microb. Genom., 1 Thompson, 1994 Tobin, 2012 Treeck, 2011, The phosphoproteomes of Plasmodium falciparum and Toxoplasma gondii reveal unusual adaptations within and beyond the parasites' boundaries, Cell Host Microbe, 10, 410, 10.1016/j.chom.2011.09.004