A Phytoplasma Closely Related to the Pigeon Pea Witches'-Broom Phytoplasma (16Sr IX) Is Associated with Citrus Huanglongbing Symptoms in the State of São Paulo, Brazil

Phytopathology - Tập 98 Số 9 - Trang 977-984 - 2008
D. C. Teixeira1, Nelson Arno Wulff, E. C. Martins, Elliot Watanabe Kitajima2, Renato Beozzo Bassanezi, A. J. Ayres, Sandrine Eveillard3, Colette Saillard3, Joseph M. Bové3
1Fundecitrus, Araraquara, CEP, Brazil.
2Universidade de São Paulo = University of São Paulo
3Génomique, développement et pouvoir pathogène

Tóm tắt

In February 2007, sweet orange trees with characteristic symptoms of huanglongbing (HLB) were encountered in a region of São Paulo state (SPs) hitherto free of HLB. These trees tested negative for the three liberibacter species associated with HLB. A polymerase chain reaction (PCR) product from symptomatic fruit columella DNA amplifications with universal primers fD1/rP1 was cloned and sequenced. The corresponding agent was found to have highest 16S rDNA sequence identity (99%) with the pigeon pea witches'-broom phytoplasma of group 16Sr IX. Sequences of PCR products obtained with phytoplasma 16S rDNA primer pairs fU5/rU3, fU5/P7 confirm these results. With two primers D7f2/D7r2 designed based on the 16S rDNA sequence of the cloned DNA fragment, positive amplifications were obtained from more than one hundred samples including symptomatic fruits and blotchy mottle leaves. Samples positive for phytoplasmas were negative for liberibacters, except for four samples, which were positive for both the phytoplasma and ‘Candidatus Liberibacter asiaticus’. The phytoplasma was detected by electron microscopy in the sieve tubes of midribs from symptomatic leaves. These results show that a phytoplasma of group IX is associated with citrus HLB symptoms in northern, central, and southern SPs. This phytoplasma has very probably been transmitted to citrus from an external source of inoculum, but the putative insect vector is not yet known.

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Tài liệu tham khảo

10.1093/nar/25.17.3389

Barros, T. S. L. 2002. Page 107 in: Caracterização molecular de Mollicutes fitopatogênicos no Brasil. Ph.D. thesis, University of Brasília, Brazil.

Bedendo, I. P., Davis, R. E., and Dally, L. 1999. Detecção e caracterização de fitoplasmas em plantas de vinca (Catharanthus roseus) e de pimenta (Capsicum frutescens) através de técnicas de duplo PCR e RFLP. (Abstr.) Summa Phytopathol. 25:197-201.

Bové J. M., 2006, J. Plant Pathol., 88, 7

10.1016/S0168-9452(02)00276-5

10.1146/annurev.phyto.41.052102.104034

10.1094/PDIS.2004.88.12.1382C

10.1093/nar/16.22.10881

10.1146/annurev.py.29.090191.000545

Da Graça, J. V., and Korsten, L. 2004. Citrus huanglongbing: Review, present status and future strategies. Pages 229-245 in: Diseases of Fruits and Vegetables. S. A. M. H. Naqvi, ed. Kluwer Academic Publishers, Dordrecht, The Netherlands.

10.1094/PHYTO.2003.93.6.644

Garnier, M., and Bové, J. M. 1996. Distribution of the huanglongbing (greening) liberibacter species in fifteen African and Asian countries. Pages 388-391 in: Proceedings of 13th Conference IOCV, Riverside, CA.

Garnier, M., and Bové, J. M. 2000. Huanglongbing in Cambodia, Laos, and Myanmar. Pages 378-380 in: Proceedings of 14th Conference IOCV, Riverside, CA.

Garnier, M., Bové, J. M., Cronje, C. P. R., Sanders, G. M., Korsten, L., and Le Roux, H. F. 2000 Presence of "Candidatus Liberibacter africanus" in the Western Cape province of South Africa. Pages 369-372 in: Proceedings of 14th Conference IOCV, Riverside, CA.

Garnier, M., Jagoueix, S., Toorawa, P., Grisoni, M., Mallessard, R., Dookun, A., Saumtally, S., Autrey, J. C., and Bové, J. M. 1996. Both huanglongbing (greening) liberibacter species are present in Mauritius and Reunion. Pages 392-394 in: Proceedings of 13th Conference IOCV, Riverside, CA.

10.1094/PHYTO.2002.92.4.384

Gottwald, T. R., da Graça, J. V., and Bassanezi, R. B. 2007. Citrus huanglongbing: The pathogen and its impact. Plant Health Progress Online publication DOI:10.1094/PHP-0906-01-RV.10.1094/PHP-0906-01-RV

10.1071/AR9791101

Halbert S. E., 2004, Entomol., 87, 330

Halbert S. E., 2004, Entomol., 87, 401

10.1006/mcpr.1999.0263

10.1099/00207713-44-3-379

10.1006/mcpr.1996.0006

10.1099/00207713-47-1-224

Kitajima, E. W., and Nome, C. F. 1999. Microscopia eletrônica em virologia vegetal. Pages 59-87 in: Métodos para Detectar Patógenos Sistêmicos. D. M. Docampo and S. L. Lenardon, eds. Córdoba IFFYVE/ INTA, Spain.

10.1093/nar/8.19.4321

Najar A., 1998, Fruits, 53, 391

10.1099/00207713-44-3-440

Sémétey, O., Gatineau, F., Bressan, A., and Boudon-Padieu, E. 2007. Characterization of a γ-3 proteobacteria responsible for the syndrome "Basses Richesses" of sugar beet transmitted by Pentostiridius sp. (Hemiptera, Cixiidae). Phytopathology 97:72-78.

10.1128/aem.62.8.2988-2993.1996

10.1071/AP05007

10.1093/molbev/msm092

10.1016/j.mcp.2004.11.002

10.1099/ijs.0.63677-0

Teixeira, D. C., Saillard, C., Eveillard, S., Danet, J. L., Ayres, A. J., and Bové, J. M. 2005. A new liberibacter species, Candidatus Liberibacter americanus sp. nov., is associated with citrus huanglongbing (greening disease) in São Paulo State, Brazil. Pages 325-340 in: Proceedings16th Conference IOCV, Riverside, CA.

10.1093/nar/22.22.4673

10.1128/jb.173.2.697-703.1991

Yamamoto, P. T., Felippe, M. R., Garbim, L. F., Coelho, J. H. C., Ximenes, N. L., Martins, E. C., Leite, A. P. R., Sousa, M. C., Abrahao, D. P., and Braz, J. D. 2006. Diaphorina citri (Kuwayama) (Hemiptera: Psyllidae): Vector of the bacterium Candidatus Liberibacter americanus. Page 96 in: Proc. of the Huanglongbing-Greening Intl. Workshop, Ribeirão Preto, SP, Brazil.

10.1099/00207713-48-1-257

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Phytopathology

Tập 98 Số 9

977-984

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