A Glut4‐Vesicle Marker Protein, Insulin‐Responsive Aminopeptidase, is Localized in a Novel Vesicular Compartment in PC12 Cells

Traffic - Tập 2 Số 8 - Trang 577-587 - 2001
Galini Thoidis1, Konstantin V. Kandror1
1Boston University School of Medicine, Boston, MA 02118, USA

Tóm tắt

Glut4‐containing vesicles represent a regulated recycling compartment in insulin‐sensitive fat and skeletal muscle cells, the nature and origin of which are not fully understood. In addition to Glut4 itself, these vesicles compartmentalize a number of proteins, at least one of which, insulin‐responsive aminopeptidase, or IRAP, is completely colocalized with Glut4 in insulin‐sensitive tissues. However, unlike Glut4, IRAP is expressed in a variety of other tissues and cell lines. Here, we explored the intracellular localization of IRAP in the rat pheochromocytoma cell line PC12. We found that this protein is present in a distinct population of slowly recycling light vesicles. By gradient centrifugations, immunoadsorption and double immunofluorescent staining, these vesicles are different from transferrin‐containing endosomes, small synaptic vesicles and secretory granules and may thus represent a novel compartment in PC12 cells. Glut4‐GFP chimera transiently expressed in PC12 cells is targeted to IRAP‐containing vesicles indicating that cotargeting of Glut4 and IRAP is not specific for adipocytes and myocytes, but is faithful in a foreign cell type. We suggest that PC12 cells may possess a novel type of a vesicular carrier that may represent the homolog of Glut4‐vesicles.

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