VIP17/MAL, a proteolipid in apical transport vesicles

FEBS Letters - Tập 377 - Trang 465-469 - 1995
Daniele Zacchetti1, Johan Peränen1, Masayuki Murata1, Klaus Fiedler1, Kai Simons1
1Cell Biology Programme, European Molecular Biology Laboratory, Postfach 102209, 69012 Heidelberg, Germany

Tóm tắt

VIP17 is a proteolipid enriched in the CHAPS‐insoluble complexes from MDCK cells, and a candidate component of the molecular machinery responsible for the sorting and targeting of proteins to the apical surface. Cloning and sequencing of the cDNA encoding the protein revealed that it is the canine homolog of the human and rat MAL proteins. Analysis by immunofluorescence microscopy of epitope‐tagged VIP17/MAL expressed transiently in BHK cells and stably in MDCK cells revealed a perinuclear, vesicular, and plasmalemmal staining. In MDCK cells the distribution was mainly in vesicular structures in the apical cytoplasm. These and other results suggest that VIP17/MAL is an important component in vesicular trafficking cycling between the Golgi complex and the apical plasma membrane.

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FEBS Letters

Tập 377

465-469

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