Analysis of cholesterol ester accumulation in macrophages by the use of digital imaging fluorescence microscopy

Krieger, 1979, Reconstituted low density lipoprotein: A vehicle for the delivery of hydrophobic fluorescent probes to cells, J. Supramol. Struct., 10, 467, 10.1002/jss.400100409 Anderson, 1980, Fluorescence visualization of receptor-bound low density lipoprotein in human fibroblast, J. Recept. Res., 1, 17, 10.3109/10799898009039253 Pitas, 1981, Acetoacetylated lipoproteins used to distinguish fibroblasts from macrophages in vitro by fluorescence microscopy, Arteriosclerosis, 1, 177, 10.1161/01.ATV.1.3.177 Craig, 1981, Low density lipoproteins reconstituted with steroids containing the nitrobenzoxadiazole fluorophore, J. Lipid Res., 22, 687, 10.1016/S0022-2275(20)37380-6 Pitas, 1983, Foam cells in explants of atherosclerotic rabbit aortas have receptors for β-very low density lipoproteins, Arteriosclerosis, 3, 2, 10.1161/01.ATV.3.1.2 Reynolds, 1985, A comparative microscopic and biochemical study of the uptake of fluorescent and 125I-labeled lipoproteins by skin fibroblasts, smooth muscle cells and peritoneal macrophages in culture, Am. J. Pathol., 121, 200 McGookey, 1983, Morphological characterization of the cholesteryl ester cycle in cultured mouse macrophage foam cells, J. Cell Biol., 97, 1156, 10.1083/jcb.97.4.1156 Kruth, 1983, Filipin-positive, oil red O-negative particles in atherosclerotic lesions induced by cholesterol feeding, Lab. Invest., 50, 87 Greenspan, 1985, Nile red: a selective fluorescent stain for intracellular lipid droplets, J. Cell Biol., 100, 965, 10.1083/jcb.100.3.965 Jaakkola, 1988, Lipoprotein uptake in primary cell cultures of rabbit atherosclerotic lesions. A fluorescence microscopic and flow cytometric study, Atherosclerosis, 69, 257, 10.1016/0021-9150(88)90022-6 Benson, 1985, Digital imaging fluorescence mircroscopy: spatial heterogeneity of photobleaching rate constants in individual cells, J. Cell. Biol., 100, 1309, 10.1083/jcb.100.4.1309 Smith, 1986, Digital Imaging fluorescence microscopy, Methods Enzymol., 129, 857, 10.1016/0076-6879(86)29109-0 Dvorin, 1986, Apolipoprotein A-IV-Determinant for binding and uptake of high density lipoproteins by rat hepatocytes, J. Biol. Chem., 261, 15714, 10.1016/S0021-9258(18)66776-6 Alaupovic, 1972, Studies on the composition and structure of lasma lipoproteins. Distribution of lipoprotein families in major density classes of normal human plasma lipoproteins, Biochim. Biophys. Acta, 260, 689, 10.1016/0005-2760(72)90018-5 Basu, 1976, Degradation of cationized low density lipoprotein and regulation of cholesterol metabolism in homozygous familial hypercholesterolemia fibroblasts, 73, 3178 Wang, 1987, Lipid composition and lipolytic activities in milk from a patient with homozygous familial hypobetalipoproteinemia, Am. J. Clin. Nutr., 45, 730, 10.1093/ajcn/45.4.730 Via, 1985, Metabolism of normal and modified low-density lipoproteins by macrophage cell lines of murine and human origin, Biochim. Biophys. Acta, 833, 417, 10.1016/0005-2760(85)90099-2 Berg, 1986, Fluorescence studies of macrophage recognition and endocytosis of native and acetylated low-density lipoprotein, Biochim. Biophys. Acta, 887, 304, 10.1016/0167-4889(86)90158-8 Cupp, 1987, Flow cytometric quantification of cholesteryl ester-containing foam cells, Exp. Mol. Pathol., 46, 40, 10.1016/0014-4800(87)90029-3 Fowler, 1985, Application of Nile red, a fluorescent hydrophobic probe, for the detection of neutral lipid deposits in tissue sections: comparison with Oil Red-O, J. Histochem. Cytochem., 33, 833, 10.1177/33.8.4020099 Greenspan, 1985, Spectrofluorometric studies of the lipid probe nile red, J. Lipid Res., 26, 781, 10.1016/S0022-2275(20)34307-8 Brown, 1983, Lipoprotein metabolism in the macrophages: implications for cholesterol deposits in atherosclerosis, Annu. Rev. Biochem., 52, 223, 10.1146/annurev.bi.52.070183.001255 Fogelman, 1988, Macrophage Lipoprotein Receptors, J. Cell. Sci., 135, 10.1242/jcs.1988.Supplement_9.7 Mitchinson, 1987, Macrophages and atherogenesis, Lancet, 2, 146, 10.1016/S0140-6736(87)92341-5 Fowler, 1985, Foam cells and atherogenesis, Ann. N.Y. Acad. Sci., 454, 79, 10.1111/j.1749-6632.1985.tb11846.x Koren, 1988, Cholesterol ester accumulation in macrophages from patients with coronary artery disease