Crystal Structure of a CAP-DNA Complex: the DNA Is Bent by 90°

American Association for the Advancement of Science (AAAS) - Tập 253 Số 5023 - Trang 1001-1007 - 1991
Steve C. Schultz1, George C. Shields1, Thomas A. Steitz2,1
1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
2Department of Chemistry, Yale University, New Haven, CT 06511.

Tóm tắt

The 3 angstrom resolution crystal structure of the Escherichia coli catabolite gene activator protein (CAP) complexed with a 30-base pair DNA sequence shows that the DNA is bent by 90°. This bend results almost entirely from two 40° kinks that occur between TG/CA base pairs at positions 5 and 6 on each side of the dyad axis of the complex. DNA sequence discrimination by CAP derives both from sequence-dependent distortion of the DNA helix and from direct hydrogen-bonding interactions between three protein side chains and the exposed edges of three base pairs in the major groove of the DNA. The structure of this transcription factor—DNA complex provides insights into possible mechanisms of transcription activation.

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Tài liệu tham khảo

10.1126/science.3187531

ANDERSON, J, COCRYSTALS OF THE DNA-BINDING DOMAIN OF PHAGE-434 REPRESSOR AND A SYNTHETIC PHAGE 434 OPERATOR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 81: 1307 (1984).

10.1038/326846a0

10.1107/S0567739474000520

10.1016/0022-2836(72)90031-9

10.1093/nar/18.24.7243

BETTER, M, SITE-SPECIFIC DNA CONDENSATION AND PAIRING MEDIATED BY THE INT PROTEIN OF BACTERIOPHAGE-LAMBDA, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 79: 5837 (1982).

Bienkowska J. Program Loliplot (1989).

10.1007/BF00775753

10.1002/j.1460-2075.1989.tb08615.x

10.1073/pnas.87.20.8165

10.1073/pnas.83.11.3746

BRUNGER A.T. COMMUNICATION.

10.1016/0022-2836(88)90211-2

Brunger A. T. Program XPLOR (1989).

BUSBY, S, POSITIVE REGULATION OF GENE-EXPRESSION BY CYCLIC-AMP AND ITS RECEPTOR PROTEIN IN ESCHERICHIA-COLI, MICROBIOLOGICAL SCIENCES 4: 371 (1987).

10.1038/255530a0

10.1126/science.6372090

DICKERSON R COMMUNICATION.

10.1080/07391102.1987.10506413

10.1016/S0022-2836(77)80132-0

10.1073/pnas.86.6.1816

10.1016/0022-2836(85)90396-1

10.1080/07391102.1987.10506370

10.1038/311232a0

10.1093/nar/18.6.1457

10.1073/pnas.84.17.6083

10.1128/jb.173.16.5024-5029.1991

10.1107/S0021889887012299

FRATINI, A.V., REVERSIBLE BENDING AND HELIX GEOMETRY IN A B-DNA DODECAMER - CGCGAATTBRCGCG, JOURNAL OF BIOLOGICAL CHEMISTRY 257: 4686 (1982).

10.1038/309327a0

Furnish G. Program Plot (1989).

10.1016/0022-2836(91)90563-L

10.1038/333824a0

10.1016/0092-8674(90)90118-X

10.1042/bj2420645

10.1016/S0022-2836(05)80124-X

10.1002/bip.1981.360200513

10.1073/pnas.84.23.8315

10.1042/bj2460227

10.1126/science.3906896

10.1126/science.3187530

KATTI S COMMUNICATION.

10.1016/0092-8674(90)90453-L

10.1093/nar/11.22.7833

10.1093/nar/17.1.317

10.1002/j.1460-2075.1986.tb04284.x

10.1038/326886a0

10.1073/pnas.85.13.4633

10.1080/07391102.1989.10507728

10.1126/science.7071602

10.1016/0092-8674(86)90814-7

10.1016/0065-227X(85)90035-8

MAJORS J.E. THESIS HARVARD U CAM (1977).

10.1016/0022-2836(84)90262-6

10.1146/annurev.bi.49.070180.005343

10.1038/290744a0

10.1002/bip.1978.360171005

10.1038/335321a0

10.1038/298443a0

PASSNER J unpublished data.

10.1126/science.2028256

Perlo A Program Szazam (version 6.2).

10.1042/bj2500897

10.1002/j.1460-2075.1984.tb02223.x

10.1080/07391102.1989.10507729

10.1107/S0108767386099622

10.1038/311532a0

Rould M. R. Program Maximage (1990).

10.1016/0022-2836(86)90452-3

Jones, T. A, Computational Crystallography: 303 (1982).

10.1016/S0022-2836(05)80128-7

SHIELDS G.C. unpublished data.

10.1016/0092-8674(80)90613-3

10.1073/pnas.73.9.3068

10.1002/j.1460-2075.1984.tb01759.x

10.1080/07391102.1983.10507500

10.1017/S0033583500005552

10.1016/0022-2836(89)90522-6

10.1073/pnas.75.1.103

10.1093/nar/16.20.9687

ULLMANN, A, ROLE OF CYCLIC-AMP IN BACTERIA, ADVANCES IN CYCLIC NUCLEOTIDE RESEARCH 15: 1 (1983).

10.1002/prot.340020404

10.1016/0022-2836(87)90315-9

WEBER, I.T., MODEL OF SPECIFIC COMPLEX BETWEEN CATABOLITE GENE ACTIVATOR PROTEIN AND B-DNA SUGGESTED BY ELECTROSTATIC COMPLEMENTARITY, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 81: 3973 (1984).

10.1038/335789a0

10.1038/308509a0

10.1073/pnas.85.17.6332

10.1073/pnas.87.12.4717

10.1093/nar/6.3.1081

10.1002/bip.360290106