<i>Lactococcus lactis,</i> an Efficient Cell Factory for Recombinant Protein Production and Secretion

Microbial Physiology - Tập 14 Số 1-3 - Trang 48-58 - 2008
Eric Morello1,2, Luis G. Bermúdez‐Humarán3, Daniel Llull4, Véronique Solé-Jamault2, N. Miraglio2, Philippe Langella3, Isabelle Poquet1
1Bactéries Lactiques et Pathogènes Opportunistes
2Prologue Biotech
3Unité de recherche d'Écologie et Physiologie du Système Digestif
4Consejo Superior de Investigaciones Cientificas = Spanish National Research Council

Tóm tắt

The use of Gram-positive bacteria for heterologous protein production proves to be a useful choice due to easy protein secretion and purification. The lactic acid bacterium <i>Lactococcus lactis</i> emerges as an attractive alternative to the Gram-positive model <i>Bacillus subtilis</i>. Here, we review recent work on the expression and secretion systems available for heterologous protein secretion in <i>L. lactis</i>, including promoters, signal peptides and mutant host strains known to overcome some bottlenecks of the process. Among the tools developed in our laboratory, inactivation of HtrA, the unique housekeeping protease at the cell surface, or complementation of the Sec machinery with <i>B. subtilis</i> SecDF accessory protein each result in the increase in heterologous protein yield. Furthermore, our lactococcal expression/secretion system, using both P<sub>Zn</sub><i>zitR,</i> an expression cassette tightly controlled by environmental zinc, and a consensus signal peptide, SP<sub>Exp4</sub>, allows efficient production and secretion of the staphylococcal nuclease, as evidenced by protein yields (protein amount/biomass) comparable to those obtained using NICE or P170 expression systems under similar laboratory conditions. Finally, the toolbox we are developing should contribute to enlarge the use of <i>L. lactis</i> as a protein cell factory.

Từ khóa


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Microbial Physiology

Tập 14 Số 1-3

48-58

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