Solid-state 29Si MAS NMR studies of diatoms: structural characterization of biosilica deposits

Springer Science and Business Media LLC - Tập 375 - Trang 630-634 - 2003
R. Bertermann1, N. Kröger2, R. Tacke1
1Institut für Anorganische Chemie, Universität Würzburg, Würzburg, Germany
2Lehrstuhl Biochemie I, Universität Regensburg, Regensburg, Germany

Tóm tắt

Four different diatom species (Chaetoceros debilis, Chaetoceros didymum, Cylindrotheca fusiformis, Nitzschia angularis) were studied by solid-state 29Si MAS NMR spectroscopy. To determine the Q2:Q3:Q4 ratios in the biosilica deposits of the diatoms, quantitative 29Si MAS NMR experiments were performed. This analysis did not reveal any differences regarding the molecular architecture of the silica (i.e. the degree of condensation of the SiOH units (2 ≡SiOH → ≡Si–O–Si≡ + H2O)) from the different diatom species. However, complete cells showed significantly smaller Q4:Q3 ratios (1.8–1.9) than extracted cell walls (2.5–2.8), indicating the existence of intracellular pools of less condensed silica.

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Springer Science and Business Media LLC

Tập 375

630-634

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