Wiley
Công bố khoa học tiêu biểu
Sắp xếp:
Microanatomy and intramural physical forces within the coronary arteries (man) Abstract The osita of the left and right coronary arteries differ as to location, the distribution of surrounding muscle bundles, and in the extension of aortic elements into the proximal segments of the vessels. The muscle of the media of the right coronary artery is composed of thick bundles describing a helical contour and arranged in repetitive segments. Multiple muscle bundles make up the media of the anterier descending and circumflex arteries, and each bundle arises separately from different quadrants of the adventitia. Contraction of the muscle bundles may generate intramural physical forces. Ostial regions, sites of nearly continual deformation from the pulse wave, develop focal physical forces. Accentuation of these physical forces may occur in certain regions and result in the alteration of the histology of the artery by depositing connective tissue elements.
Wiley - Tập 153 Số 3 - Trang 233-241 - 1965
The junctional specializations of sertoli cells in the seminiferous epithelium Abstract Attention is directed to an unusual type of junctional complex between Sertoli cells in the seminiferous epithelium. The space between the membranes of adjoining cells is narrowed to 70–90 Å over large areas of their contact surfaces. In the superficial cytoplasm of each cell is an extensive cisterna of the endoplasmic reticulum, parallel to the membrane and 400–600 Å from it. Spaced at more or less regular intervals in the thin layer of cytoplasm between the cisterna and the cell membrane are periodic densities that appear to be band‐like aggregations of fine filaments. The sub‐surface cisternae are smooth‐contoured on the side toward the cell membrane but bear ribosomes on the side facing the cytoplasm. The possible significance of these distinctive junctions is discussed in relation to the support of the germ cells and the coordination of the developmental events in the cycle of the seminiferous epithelium.
Wiley - Tập 158 Số 2 - Trang 207-221 - 1967
Sperm/egg interaction: The specificity of human spermatozoa Abstract Human spermatozoa display unusually limited affinities in their interaction with oocytes of other species. They adhered to and, when capacitated, penetrated the vestments of the oocyte of an ape‐the gibbon, Hylobates lar‐ both in vivo and in vitro. On the other hand, human spermatozoa would not even attach to the zona surface of sub‐hominoid primate (baboon, rhesus monkey, squirrel monkey), nor to the non‐primate eutherian oocytes tested. Among the apes the gibbon stands furthest from man. Thus, although the specificity of human spermatozoa is not confined to man alone, it probably is restricted to the Hominoidea. This study also suggests that the evolution of man and perhaps the other hominids has been accompanied by a restrictive change in the nature of the sperm surface which has limited and made more specific the complementary surface to which their spermatozoa may adhere. For the failure of human spermatozoa to attach to the zona surface of all non‐hominoid oocytes stands in contrast to the behaviour of spermatozoa of the several other mammals studied which, in most combinations, adhered readily to foreign oocytes, including those of man. Taxonomically, the demonstration of a compatibility between the gametes of man and gibbon, not shared with cercopithecids, constitutes further evidence for inclusion of the Hylobatidae within the Hominoidea.
Wiley - Tập 188 Số 4 - Trang 477-487 - 1977
Degeneration of germ cells in normal, hypophysectomized and hormone treated hypophysectomized rats Abstract In normal adult rats some germ cells degenerate at several vulnerable steps of spermatogenesis. These are the type A spermatogonia, midpachytene spermatocytes, primary and secondary spermatocytes which degenerate during their respective maturation divisions and step 7 and 19 spermatids. In the present study, these degenerating cells were examined under the electron microscope, and their frequency was determined in toluidine blue stained semithin sections of testes from normal, hypophysectomized (at 5.5 days after operation) and hypophysectomized rats injected with FSH and LH separately or in combination. With the exception of the step 19 spermatids, the degenerating germ cells underwent necrosis in vacuolated spaces delimited by Sertoli cells. In the case of the affected step 19 spermatids, an apical cytoplasmic process of the Sertoli cell initially ensheathed a long segment of their flagellum, and then each degenerating cell was drawn deep in the seminiferous epithelium where it was phagocytozed by the Sertoli cell. Soon after hypophysectomy the incidence of degenerating mid‐pachytene spermatocytes, step 7 and 19 spermatids which are present in stages VII or VIII of the cycle of the seminiferous epithelium, increased significantly. In contrast the number of degenerating primary or secondary spermatocytes during the meiotic divisions seen in stage XIV of the cycle or of any other germinal cell was not significantly modified. While the injection of FSH alone had no influence on the number of degenerating cells in hypophysectomized rats, injections of LH at the two doses administered (0.7 μg or 20 μg) reduced significantly the number of degenerating cells seen in stages VII‐VIII of the cycle; combined injections of FSH and LH (20 μg) reduced the number of these degenerating cells to the normal low values. Thus it appeared that the mid‐pachytene spermatocytes and the step 7 and 19 spermatids, all present in the adluminal compartment of the seminiferous epithelium in stages VII or VIII of the cycle, were more sensitive to the presence of absence of gonadotropic hormones than the other germ cells present in the seminiferous epithelium.
Wiley - Tập 187 Số 3 - Trang 347-365 - 1977
The fine structure of the monkey (<i>Macaca</i>) sertoli cell and its role in maintaining the blood‐testis barrier Abstract The monkey Sertoli cell, a tall columnar cell, extends from the basement membrane of the seminiferous epithelium to the tubule lumen. Its nucleus occupies a basal position and reveals extensive nuclear envelope infoldings. A zone of fine filaments, approximately 0.5 μ in thickness, invests the nucleus and appears to prevent other cell organelles from approaching it. The basal cytoplasm is characterized by numerous mitochondria and abundant smooth endoplasmic reticulum. Lipid droplets, 3 to 4 μ in diameter, membrane‐limited dense bodies of various shapes and densities, Golgi cisternae, scattered free ribosomes and parallel profiles of rough endoplasmic reticulum are common. The more apical portions of the cell contain longitudinally oriented microtubules and rod‐shaped mitochondria, but other organelles are rare. The seminiferous tubules of monkeys are surrounded by three to five circumferentially arranged cells that overlap each other but are separated by intercellular spaces of at least 300 to 400 Å. Tracers such as horseradish peroxidase and lanthanum nitrate injected intravascularly readily pass between the peritubular cells and enter the germinal epithelium. Within the epithelium the tracers outline the spermatogonia and early spermatocytes by permeating the surrounding intercellular spaces. Further penetration toward the tubule lumen is effectively prevented by the occluding tight junctions joining adjacent Sertoli cells. Thus, in monkeys the peritubular epithelioid cells do not impede vascularly introduced tracers from penetrating into the germinal epithelium. The only morphological component of the blood‐testis barrier in the macaque appears to be the Sertoli‐Sertoli occluding junction.
Wiley - Tập 175 Số 4 - Trang 639-656 - 1973
Satellite cells as the source of nuclei in muscles of growing rats Abstract The source of the new nuclei appearing during the growth of muscle fibers was examined in the tibialis anterior muscle of young Sherman rats (14–17 days of age) using radioautography at various intervals after a single injection of a small, non‐toxic dose of 3 H‐thymidine (2 μCi/g body weight). Two techniques were employed: (1) labeled nuclei were detected in 1 μ thick radioautographs examined in the light microscope, and identified by simultaneous electron microscope examination of an adjacent section. The nuclei were then classified either as “true” muscle nuclei (within the plasmalemma of the fibers) or as belonging to “satellite cells” (which are mononucleated cells with scanty cytoplasm wedged between plasmalemma and basement membrane). (2) Muscle fibers freed by collagenase digestion were radioautographed one hour after 3 H‐thymidine injection in order to determine the total number of labeled nuclei (true muscle nuclei plus those of satellite cells) per unit length of fiber. Certain nuclei within the basement membrane of muscle fibers are labeled one hour after 3 H‐thymidine and, therefore, synthesize DNA. The electron microscope demonstrates that these nuclei invariably belong to satellite cells, never to true muscle nuclei. Furthermore, the total number of labeled nuclei per unit length of fiber doubles between 1 and 24 hours; and, therefore, the labeled satellite cell nuclei undergo mitosis. Following mitosis, half of the daughters of satellite cells are incorporated into the fibers to become true muscle nuclei. The remaining half divides again later; and half of their daughter cells are incorporated. Thus, satellite cells in young rats divide repeatedly and function as a source of true muscle nuclei.
Wiley - Tập 170 Số 4 - Trang 421-435 - 1971
Myogenic cell formation in regenerating rat skeletal muscle injured by mincing II. An autoradiographic study Abstract Myonuclei and satellite cell nuclei were differentially labelled with 3 H‐thymidine in uninjured skeletal muscle of young rats and then traced autoradiographically at intervals after mincing the radioactive hindlimb muscles to determine the source of regenerating presumptive myoblasts. Labelled nuclei were detected by light microscopic examination of 1‐m̈ thick autoradiographs and identified by electron microscopic examination of an adjacent section. Repeated injections of 3 H‐thymidine during fetal and neonatal development, followed by a 4‐ to 5‐week maturation period, resulted in labelling of 20% of the myonuclei. Satellite cells were not observed to be labelled in this series. Eight to sixteen hours after mincing, 20% of the pyknotic myonuclei were labelled, whereas none of the regenerating presumptive myoblasts appeared labelled. A single injection of 3 H‐thymidine administered to 18‐day‐old rats, followed by sacrifice within ten hours, resulted in labelling of 23% of the satellite cell nuclei. Myonuclei were not observed to be labelled in this series. Eight to sixteen hours after mincing, silver grains were detected over both pyknotic and regenerating cell nuclei. These experiments indicate that many satellite cells survive muscle injury and transplantation to become regenerating myogenic cells at a time when most, if not all, myonuclei are undergoing pyknosis.
Wiley - Tập 188 Số 2 - Trang 201-217 - 1977
Immunological and morphological effects of vasectomy in the rabbit Abstract Half of the rabbits developed antisperm antibodies (measured by either indirect immunofluorescence or sperm immobilization tests) after either a unilateral or bilateral vasectomy. The raised antibody levels, particularly six months or longer after vasectomy, often accompanied patchy orchitis. Seminiferous tubules from such animals exhibited sloughed, multinucleated, and immature germinal cells which were engulfed by phagocytic cells. Mononuclear infiltrates were occasionally present. The basal lamina infolded and thickened by means of supernumerary layers and appeared to be endocytosed by cells of the seminiferous tubules. Four months after vasectomy, numerous phagocytic cells were seen to migrate through the intact epithelium of zone 1 in the caput epididymidis, and were particularly prevalent in animals that exhibited testicular damage. These macrophages may serve to present sperm antigens to lymphocytes.
Wiley - Tập 188 Số 3 - Trang 339-350 - 1977
Hominid cranial bone structure: A histological study of Omo 1 specimens from Ethiopia using different microscopic techniques Abstract The microstructure of a hominid cranial vault has not previously been studied to determine its tissue histology, and differences in comparison with that of modern humans. We selected the parietals of Omo‐Kibish 1, regarded as one of the oldest (about 130,000 years old) anatomically modern humans, and Omo 1 (Howell), which is a very recent human (about 2,000 years old)—both from the same area of Ethiopia. A combination of macrophotography, polarizing microscopy in the incident and transmission illumination mode, and confocal laser scanning microscopy (CLSM) was employed to examine thin sections, as well as polished and unpolished block faces of unembedded bone fragments, to minimize specimen destruction as much as possible. The methods enabled remarkably detailed information on bone microstructure and remodeling to be gleaned from tiny fragments of bone. The best method for examining fossilized human bones was shown to be that of incident light microscopy, which was the least destructive while producing the most amount of information. Unless the above methods are used, bone‐filling minerals, such as calcite, can cause erroneous estimations of bone thickness, as observations with the naked eye or even a magnifying glass cannot determine the limit between the cortex and the diploe. This is particularly important for sciences such as paleoanthropology, in which, for instance, a thick cranial bone of Homo erectus may be confused with a pathological one of H. sapiens and vice versa. Cross sections of parietal bones revealed differences between Omo‐Kibish 1 and Omo 1 (Howell) in diploic histology and in the relative thickness between the cortex and diploe, with the former specimen having an H. erectus ratio despite its H. sapiens gross anatomy. Omo‐Kibish 1 may still retain some affinities with H. erectus despite its being classified as H. sapiens. Newly described histological structures, such as the reverse type II osteons, the multicanalled osteons, and the osteocytomata are presented here. A modern human skeletal anatomy does not necessarily imply a modern human cranial bone histology. The outer circumferential lamellae of cranial bones are in essence growth lines. Cranial histology of hominids may provide useful information concerning their taxonomy and life history, including such factors as growth rate, developmental stress, and diet. Anat Rec 267:52–59, 2002. © 2002 Wiley‐Liss, Inc.
Wiley - Tập 267 Số 1 - Trang 52-59 - 2002
Tổng số: 89
- 1
- 2
- 3
- 4
- 5
- 6
- 9